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As the collapse of the vessels increased, lining cells became thicker and more rugged, endothelial fenestrations disappeared, and 10 nm filaments aggregated. Simultaneously, perivascular connective tissue material was separated from the dermal element in which the vessels were embedded and appeared as a homogenous areola around the endothelial tube. Basal lamina appeared folded and partially multilaminated around the vascular circumference, particularly in the venous segment of the microvasculature; these are considered to be the definite characteristics of dermal capillaries. In contrast to the skin regions with thin epidermis, the vessels in \u201cwell developed\u201d dermal papillae did not collapse and bore close similarities to the perfused ones. The present study indicates that most of the so\u2010called characteristics of dermal capillaries in biopsy skin is attributable to artifacts following the removal of the skin before fixation.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1986.tb02952.x","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:42:44Z","timestamp":1428709364000},"page":"339-344","source":"Crossref","is-referenced-by-count":3,"title":["Fine Structural Deformation of the Dermal Capillary Following Immersion Fixation Procedure"],"prefix":"10.1111","volume":"13","author":[{"given":"Shuhei","family":"Imayama","sequence":"first","affiliation":[{"name":"Department of Dermatology Faculty of Medicine, Kyushu University  Fukuoka Japan (Director: Prof. H. 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For this purpose, an <jats:italic>in vitro<\/jats:italic> phototoxic index was designed as the reciprocal of the product of minimal growth inhibition concentration and UVA dose in <jats:italic>Candida<\/jats:italic>, growth inhibition. Obtained results showed that the 11 drugs tested could be ranked by index as follows: 8\u2010methoxypsoralen (50.0) \u00bb prochlorperazine (5.0) &gt; tiaprofenic acid (2.0) &gt; chlorpromazine (1.25) &gt; nalidixic acid (1.0) &gt; naproxen (0.5) &gt; fluorouracil (0.25)=ofloxacin (0.25) &gt; enoxacin (0.2). Tegafur and piroxicam showed no inhibition.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1988.tb01202.x","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:33:32Z","timestamp":1428708812000},"page":"523-526","source":"Crossref","is-referenced-by-count":3,"title":["Phototoxic Index of Drug Determined by <i>Candida<\/i> Growth Inhibition"],"prefix":"10.1111","volume":"15","author":[{"given":"Etsuro","family":"Horikawa","sequence":"first","affiliation":[{"name":"Department of Dermatology Teikyo University School of Medicine Ichihara Hospital  Japan (Director: Prof. T. Miura)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Miura","sequence":"additional","affiliation":[{"name":"Department of Dermatology Teikyo University School of Medicine Ichihara Hospital  Japan (Director: Prof. T. 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The clinical diagnoses were mixed connective tissue disease (7 patients), systemic lupus erythematosus (5 patients), scleroderma (3 patients) and rheumatoid arthritis (1 patient). Detailed serologic evaluation in 13 of the 16 patients revealed positive rheumatoid factor (7 patients), antinuclear antibody (13 patients), anti\u2010DNA (2 patients), anti\u2010n\u2010RNP (11 patients), anti\u2010Sm (2 patients), anti\u2010SS\u2010A (3 patients) and anti\u2010RANA (5 patients). Anti\u2010SS\u2010B and anti\u2010Scl\u201070 were absent in all. Epidermal nuclear immunofluorescence on skin biopsy correlated with serum anti\u2010n\u2010RNP and is a useful marker for mixed connective tissue disease. However, it did not appear to serve as a specific marker for any one connective tissue disease. 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The number of viable melanoma cells was decreased at 10 hrs post irradiation in parallel to the increase in UVA dose (10<jats:sup>4<\/jats:sup>~10<jats:sup>7<\/jats:sup> ergs\/cm<jats:sup>2<\/jats:sup>). After ten hours, the cultured cells entered the logarithmic growth phase. The doubling time of the cells exposed to the low dose of UVA was similar to that of nonirradiated cells, but the exposure to higher doses of UVA (10<jats:sup>5<\/jats:sup>~10<jats:sup>6<\/jats:sup> ergs\/cm<jats:sup>2<\/jats:sup>) elongated the doubling time of these cells. Still higher doses of UVA exposure (10<jats:sup>7<\/jats:sup> ergs\/cm<jats:sup>2<\/jats:sup>) caused no further elongation of doubling time.<\/jats:p><jats:p>The tyrosinase activity of these melanoma cells was also measured using an oxygen electrode. The tyrosinase activity of each irradiated cell at ten hours was significantly increased, but no significant increase of tyrosinase activity per mg protein was observed, since protein content per cell increased at the same time. The increase in tyrosinase activity was still observed after ten hours in cells exposed to higher amounts of UVA. These results suggest that UVA repressed cell division and induced protein synthesis to prepare for melanogenesis.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1982.tb01080.x","type":"journal-article","created":{"date-parts":[[2015,4,11]],"date-time":"2015-04-11T00:20:06Z","timestamp":1428711606000},"page":"409-412","source":"Crossref","is-referenced-by-count":1,"title":["EFFECT OF UVA IRRADIATION ON B16 MELANOMA CELLS IN MONOLAYER CULTURE"],"prefix":"10.1111","volume":"9","author":[{"given":"Shigeaki","family":"Shono","sequence":"first","affiliation":[{"name":"Department of Dermatology (Director: K. 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Wchshr."}],"container-title":["The Journal of 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Journal of Dermatology"],"published-print":{"date-parts":[[1977,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Three cases of erythropoietic protoporphyria are reviewed. The three patients constitute members of one family. The protoporphyrin content of the red blood cells was high, but porphyrins and their precursors in the urine and faeces were not excessive. Other normal members of the family did not reveal high protoporphyrin content in the red blood cells. Clinical symptoms were itching, swelling, shallow depressed scars and waxy yellow discoloration on the face and brown pigmentation and thickness on the back of the hands after exposure to the sun. The microscopic findings from skin biopsy specimens of the lesions resembled changes of the lipoid proteinosis.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1977.tb01018.x","type":"journal-article","created":{"date-parts":[[2015,4,11]],"date-time":"2015-04-11T00:37:42Z","timestamp":1428712662000},"page":"85-89","source":"Crossref","is-referenced-by-count":2,"title":["ERYTHROPOIETIC PROTOPORPHYRIA"],"prefix":"10.1111","volume":"4","author":[{"given":"Shozaburo","family":"Ozasa","sequence":"first","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoso","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motomichi","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuichi","family":"Inada","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoko","family":"Yanai","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Takagaki","sequence":"additional","affiliation":[{"name":"Department of Dermatology Hiroshima University School of Medicine  Kasumi 1\u20102\u20103 Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00438257"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(61)92427-8"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(63)90144-X"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1001\/archderm.1974.01630070026004","article-title":"Erythropoietic protoporphyria, A clinical study based on 29 cases in 14 families","volume":"110","author":"Schmidt H.","year":"1974","journal-title":"Arch. 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It was found that <jats:italic>\u03b3<\/jats:italic>\u2010GT activity was present in both the epidermis and the dermis, being more active in the former.<\/jats:p><jats:p>The optimum pH for the enzyme was 8.5\u20139.0 in Tris buffer, which is similar to that for the rat kidney and human serum enzymes. It was also revealed that glycylglycine was the most effective activator of the enzyme. Some activation was also observed in the presence of L\u2010glutamine. But L\u2010methionine, L\u2010homoserine, L\u2010glutamic acid, L\u2010arginine, L\u2010aspartic acid, glycine and L\u2010valine inhibited the activity, suggesting that these amino acids do not act as acceptors of the <jats:italic>\u03b3<\/jats:italic>\u2010glutamyl moiety. The enzyme was remarkably inhibited by bromsulphalein, oxidized glutathione, and by L\u2010serine in the presence of borate. These inhibitions were more severe than thoes observed with rat kidney and human serum enzymes.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1979.tb01878.x","type":"journal-article","created":{"date-parts":[[2015,4,11]],"date-time":"2015-04-11T00:30:39Z","timestamp":1428712239000},"page":"39-45","source":"Crossref","is-referenced-by-count":1,"title":["GAMMA\u2010GLUTAMYL TRANSPEPTIDASE ACTIVITY IN HUMAN SKIN"],"prefix":"10.1111","volume":"6","author":[{"given":"Young Pio","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Dermatology Chon\u2010Nam University Medical School and Chon\u2010Nam University Hospital  Kwang\u2010Ju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johng Bong","family":"Kahng","sequence":"additional","affiliation":[{"name":"Department of Dermatology Chon\u2010Nam University Medical School and Chon\u2010Nam University Hospital  Kwang\u2010Ju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"411","article-title":"Metabolism of glutathione. Hydrolysis by tissues of the rat","volume":"173","author":"Binkley F.","year":"1948","journal-title":"J. Biol. 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The tumor mass had infiltrated toward the subcutaneous fatty tissue and had tubular structures composed of atypical squamous cells in numerous places. At the border of the tumor, there were histological features of the acantholytic type of solar keratosis, and apparent solar elastosis was observed beneath the normal epidermis surrounding the tumor mass. Metastases to regional lymph nodes occurred a year and a half after excision of the tumor and the patient died.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1986.tb02958.x","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:42:44Z","timestamp":1428709364000},"page":"377-380","source":"Crossref","is-referenced-by-count":1,"title":["A Case of Acantholytic Squamous Cell Carcinoma Derived from the Acantholytic Type of Solar Keratosis"],"prefix":"10.1111","volume":"13","author":[{"given":"Noriko","family":"Uchiyama","sequence":"first","affiliation":[{"name":"The Uchiyama Dermatological Clinic  Hotaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuko","family":"Shindo","sequence":"additional","affiliation":[{"name":"The Uchiyama Dermatological Clinic  Hotaka Japan"},{"name":"Department of Dermatology, Faculty of Medicine Shinsyu University  Matsumoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1947.01520080017002"},{"key":"e_1_2_1_3_1","first-page":"511","volume-title":"A Guide to Dermatohistopathology","author":"Pinkus H","year":"1976"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02069898"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1965.01600180027004"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1001\/archderm.1979.04010060011017","article-title":"Metastases in dermatological patients with squamous cell carcinoma","volume":"115","author":"M\u00f8ller R","year":"1979","journal-title":"Arch Dermatol"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4362.1979.tb01903.x"},{"key":"e_1_2_1_8_1","first-page":"516","article-title":"Metastatic potential of biologic variants of skin squamous cell carcinoma","volume":"69","author":"Cox FH","year":"1982","journal-title":"J Florida M A"},{"key":"e_1_2_1_9_1","first-page":"54","article-title":"A case of adenoid squamous cell carcinoma with leukoderma","volume":"16","author":"Saito Y","year":"1974","journal-title":"Hifuka Rinsho"},{"key":"e_1_2_1_10_1","first-page":"730","article-title":"Adenoid squamous cell carcinoma resulting from chronic pyoderma","volume":"28","author":"Nakamura K.","year":"1974","journal-title":"Rinsho Hifuka"},{"key":"e_1_2_1_11_1","first-page":"295","article-title":"Pseudoglandular squamous cell carcinoma","volume":"25","author":"Yamazaki S","year":"1983","journal-title":"Hifuka Rinsho"}],"container-title":["The Journal of Dermatology"],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1346-8138.1986.tb02958.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1346-8138.1986.tb02958.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T16:56:47Z","timestamp":1697821007000},"score":0.0,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1346-8138.1986.tb02958.x"}},"issued":{"date-parts":[[1986,10]]},"references-count":10,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1986,10]]}},"alternative-id":["10.1111\/j.1346-8138.1986.tb02958.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1346-8138.1986.tb02958.x","archive":["Portico"],"ISSN":["0385-2407","1346-8138"],"issn-type":[{"value":"0385-2407","type":"print"},{"value":"1346-8138","type":"electronic"}],"published":{"date-parts":[[1986,10]]}},{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T09:11:41Z","timestamp":1697965901560},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T00:00:00Z","timestamp":1428537600000},"content-version":"vor","delay-in-days":9869,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human Langerhans cell free epithelia can be cultured <jats:italic>in vitro<\/jats:italic>, and then can be used as epidermal allografts (EAG) without evidence of rejection. We studied the cellular basis of this phenomenon with mixed epidermal cell lymphocyte reactions (MELR). The capacity of donor\u2010derived epidermal cells to stimulate allogeneic control or recipient cells was abolished when stimulatory cells were Langerhans cell\u2010free cultured keratinocytes and respondors were obtained prior to grafting. Donor\u2010type cultured keratinocytes were able to induce a low response by host\u2010derived cells in assays conducted 2, 4 and 6 weeks after grafting, but not thereafter. They were unable to stimulate allogeneic cells unrelated to the recipient. The ability of crude epidermal suspensions with 2\u20134% Langerhans cells to stimulate host\u2010derived cells did not increase with time after grafting. No secondary type MELR could be evidenced, suggesting that grafting of EAG did not induce an <jats:italic>in vivo<\/jats:italic> immunization to class I antigens expressed by cultured epidermal cells. Lastly, host\u2010derived Langerhans cells were not able to restore the allostimulatory ability of Langerhans cell free epidermal cells from EAG donors when tested against host\u2010derived cells. This suggests that host\u2010derived Langerhans cells which colonize the grafts during the first few weeks following grafting cannot act in the presentation of foreign keratinocyte\u2010bound antigens, which may account for the absence of rejection noted.<\/jats:p>","DOI":"10.1111\/j.1346-8138.1988.tb03660.x","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T23:28:31Z","timestamp":1428708511000},"page":"104-110","source":"Crossref","is-referenced-by-count":0,"title":["Lympho\u2010epidermal Interactions in Patients Receiving Human Langerhans cell Free Cultured Epidermal Allografts as a Skin Substitute"],"prefix":"10.1111","volume":"15","author":[{"given":"Aicha","family":"Demidem","sequence":"first","affiliation":[{"name":"Inserm U 209, Pavilion R, Hopital E. Herriot  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Faure","sequence":"additional","affiliation":[{"name":"Inserm U 209, Pavilion R, Hopital E. Herriot  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Mauduit","sequence":"additional","affiliation":[{"name":"Inserm U 209, Pavilion R, Hopital E. Herriot  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Rimokh","sequence":"additional","affiliation":[{"name":"Inserm U 209, Pavilion R, Hopital E. Herriot  Lyon France"},{"name":"Laboratoire d'h\u00e9matologie et de cytog\u00e9n\u00e9tique (Pr. Germain), Hopital E. Herriot  Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Thivolet","sequence":"additional","affiliation":[{"name":"Inserm U 209, Pavilion R, Hopital E. 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Specific fluorescence was observed in epithelioid cells in the sarcoidosis granulomas examined. However, it was not found in any other granulomatous diseases observed as controls, which included granuloma annulare, foreign body granuloma and lupus miliaris disseminatus faciei. These results indicate that ACE is specifically localized in the epithelioid cells in cutaneous granuloma lesions of sarcoidosis; in one case, it was also observed in Langhans giant cells. Therefore, it is suggested that examination for ACE in cutaneous lesions using an immunofluorescent technique is very useful for the diagnosis of sarcoidosis in the skin. 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