{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T00:17:13Z","timestamp":1775693833216,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2018,9,26]],"date-time":"2018-09-26T00:00:00Z","timestamp":1537920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18K14376"],"award-info":[{"award-number":["18K14376"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008608","name":"Sumitomo Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010157","name":"Noda Institute for Scientific Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010157","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemBioChem"],"published-print":{"date-parts":[[2018,10,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cytochrome P450 enzymes are heme\u2010containing monooxygenases that exhibit potential as biocatalysts for practical applications. The <jats:italic>Escherichia coli<\/jats:italic> expression system is frequently used for biocatalyst production; however, heterologous production of hemeproteins in their holo form is difficult due to insufficient heme synthesis by the host. In this study, 5\u2010aminolevulinic acid synthase (ALAS) from <jats:italic>Rhodobacter capsulatus<\/jats:italic> is used to accelerate intracellular heme biosynthesis in <jats:italic>E.\u2005coli<\/jats:italic>; this demonstrates that coexpression of the ALAS gene (<jats:italic>ALAS<\/jats:italic>) improves the heterologous production of cytochrome P450, CYP119, from <jats:italic>Sulfolobus acidocaldarius<\/jats:italic>. Coexpression of <jats:italic>ALAS<\/jats:italic> increased the amount of heterologous CYP119 isolated and the ratio of its holo form. The ratio of holo\u2010CYP119 resulting from the coexpression of <jats:italic>ALAS<\/jats:italic> in <jats:italic>E.\u2005coli<\/jats:italic> was 99\u2009%, whereas that from cells expressing CYP119 exclusively was 66\u2009%. Coexpression of <jats:italic>ALAS<\/jats:italic> is a promising alternative for the efficient heterologous production of hemeproteins by using <jats:italic>E.\u2005coli<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cbic.201800331","type":"journal-article","created":{"date-parts":[[2018,8,12]],"date-time":"2018-08-12T21:54:32Z","timestamp":1534110872000},"page":"2156-2159","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Coexpression of 5\u2010Aminolevulinic Acid Synthase Gene Facilitates Heterologous Production of Thermostable Cytochrome P450, CYP119, in Holo Form in <i>Escherichia coli<\/i>"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6342-1924","authenticated-orcid":false,"given":"Yuki","family":"Honda","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}]},{"given":"Kii","family":"Nanasawa","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}]},{"given":"Hiroshi","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Science Nara Women's University  Kitauoyanishi-machi Nara 630-8506 Japan"}]}],"member":"311","published-online":{"date-parts":[[2018,9,26]]},"reference":[{"key":"e_1_2_2_1_1","unstructured":"\u00a0"},{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.01.029"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2011.06.012"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2016.02.018"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2013.07.017"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2340"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1016"},{"key":"e_1_2_2_8_1","unstructured":"\u00a0"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.2781"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201801592"},{"key":"e_1_2_2_10_3","doi-asserted-by":"publisher","DOI":"10.1002\/ange.201801592"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131793"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2011.05.003"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2005.07.006"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00290359"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2007.09.039"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.8.4875-4883.2003"},{"key":"e_1_2_2_17_1","unstructured":"\u00a0"},{"key":"e_1_2_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00322-5"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9584"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200700450"},{"key":"e_1_2_2_21_1","unstructured":"\u00a0"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1193478"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004281200"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)82244-3"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00085a017"},{"key":"e_1_2_2_26_1","unstructured":"\u00a0"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja017174g"},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.19.14112"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA04626A"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201000028"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.121"}],"container-title":["ChemBioChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcbic.201800331","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/chorus\/v1\/articles\/10.1002%2Fcbic.201800331","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cbic.201800331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T12:42:29Z","timestamp":1760272949000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cbic.201800331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,26]]},"references-count":32,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2018,10,18]]}},"alternative-id":["10.1002\/cbic.201800331"],"URL":"https:\/\/doi.org\/10.1002\/cbic.201800331","archive":["Portico"],"relation":{},"ISSN":["1439-4227","1439-7633"],"issn-type":[{"value":"1439-4227","type":"print"},{"value":"1439-7633","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,26]]},"assertion":[{"value":"2018-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}