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However, detailed understanding of the effect of the mechanical cues on cellular physiology is lacking. To meet this limitation, we have designed a system, which enables monitoring of living cells by high-resolution light microscopy during mechanical stimulation by HF vibration or mechanical impacts. The system consists of a commercial speaker, and a 3D printed sample vehicle and frame. The speaker moves the sample in the horizontal plane, allowing simultaneous microscopy. The HF vibration (30\u2013200\u00a0Hz) performances of two vehicles made of polymer and aluminum were characterized with accelerometer. The mechanical impacts were characterized by measuring the acceleration of the aluminum vehicle and by time lapse imaging. The lighter polymer vehicle produced higher HF vibration magnitudes at 30\u201350\u00a0Hz frequencies than the aluminum vehicle. However, the aluminum vehicle performed better at higher frequencies (60\u201370\u00a0Hz, 90\u2013100\u00a0Hz, 150\u00a0Hz). Compatibility of the system in live cell experiments was investigated with epithelial cells (MDCKII, expressing Emerald-Occludin) and HF (0.56<jats:bold><jats:italic>G<\/jats:italic><\/jats:bold><jats:sub>peak,<\/jats:sub>30\u00a0Hz and 60\u00a0Hz) vibration. Our findings indicated that our system is compatible with high-resolution live cell microscopy. Furthermore, the epithelial cells were remarkable stable under mechanical vibration stimulation. To conclude, we have designed an inexpensive tool for the studies of cellular biophysics, which combines versatile in vivo like mechanical stimuli with live cell imaging, showing a great potential for several cellular applications.<\/jats:p>","DOI":"10.1007\/s12553-019-00382-9","type":"journal-article","created":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T06:01:16Z","timestamp":1572588076000},"page":"87-99","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mechanical impact stimulation platform tailored for high-resolution light microscopy"],"prefix":"10.1007","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9486-7569","authenticated-orcid":false,"given":"Heidi T.","family":"Halonen","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1850-3055","authenticated-orcid":false,"given":"Jari A.K.","family":"Hyttinen","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4351-8697","authenticated-orcid":false,"given":"Teemu O.","family":"Ihalainen","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2019,10,31]]},"reference":[{"key":"382_CR1","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.devcel.2005.12.006","volume":"10","author":"AW Orr","year":"2006","unstructured":"Orr AW, Helmke BP, Blackman BR, Schwartz MA. 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