{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:59:38Z","timestamp":1753876778791,"version":"3.41.2"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007675","name":"International Center for Genetic Engineering and Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007675","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001474","name":"Singapore-MIT Alliance for Research and Technology Centre","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001474","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Many plant bacterial pathogens monitor their group behaviour and their population density via production of N-acyl homoserine lactone signals which regulate the expression of several genes via the LuxI\/R homologs. This regulatory network, termed quorum sensing (QS), is present in the soybean bacterial pathogen Pseudomonas savastanoi pv glycinea (Psg). The sequenced genomes of two strains of Psg, race 4 and B076, contain an N-acyl homoserine lactone (AHL) based LuxI\/R QS system named AhlI\/R. While studying the QS system of Psg strains race 4 and B076 isolated in USA, LMG5066 in New Zealand and IBSBF355 in Brazil, we found that B076, LMG5066 and IBSBF355 possess a point mutation in the ahlR gene that causes a frameshift resulting in a truncated AhlR protein. Psg race 4 does not possess the mutation in ahlR and the QS system is functional. The same mutation in the ahlR gene was found to be also present in 9 of 19 Psg strains isolated from diseased soybean in Illinois. Phenotypic analysis of strains showed that swarming motility is repressed whereas phosphate solubilisation was activated by QS in Psg. Analysing the secretome, we also found that four proteins were under QS regulation.<\/jats:p>","DOI":"10.1093\/femsle\/fnz149","type":"journal-article","created":{"date-parts":[[2019,7,4]],"date-time":"2019-07-04T16:18:16Z","timestamp":1562257096000},"source":"Crossref","is-referenced-by-count":3,"title":["Many plant pathogenic <i>Pseudomonas savastanoi<\/i> pv <i>glycinea<\/i> isolates possess an inactive quorum sensing <i>ahlR<\/i> gene via a point mutation"],"prefix":"10.1093","volume":"366","author":[{"given":"Giuliano","family":"Degrassi","sequence":"first","affiliation":[{"name":"Industrial Biotechnology Group, IBioBA-ICGEB, Godoy Cruz 2390, Buenos Aires, Argentina"}]},{"given":"Valentina","family":"Mortato","sequence":"additional","affiliation":[{"name":"Industrial Biotechnology Group, IBioBA-ICGEB, Godoy Cruz 2390, Buenos Aires, Argentina"}]},{"given":"Giulia","family":"Devescovi","sequence":"additional","affiliation":[{"name":"Bacteriology Group, ICGEB, Padriciano 99, I-34149 Trieste, Italy"}]},{"given":"Rodrigo","family":"Hoshino","sequence":"additional","affiliation":[{"name":"Agronomy Department, Londrina State University, Londrina 10.011, Parana, Brasil"}]},{"given":"Tiyakhon","family":"Chatnaparat","sequence":"additional","affiliation":[{"name":"Department of Crop Sciences, 288 E R Madigan Laboratory, 1201 W. Gregory Dr., Urbana, IL 61801, USA"}]},{"given":"Milan","family":"Kojic","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering (IMGGE), V. Stepe 444a, P.O. Box 23, 11010 Belgrade, Serbia"}]},{"given":"Valeria","family":"Carpentieri-Pipolo","sequence":"additional","affiliation":[{"name":"Embrapa Trigo, Rodovia BR-285, Km 294 PO Box 3081, 99050\u2013970, Passo Fundo, RS Brazil"}]},{"given":"Youfu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Crop Sciences, 288 E R Madigan Laboratory, 1201 W. Gregory Dr., Urbana, IL 61801, USA"}]},{"given":"Vittorio","family":"Venturi","sequence":"additional","affiliation":[{"name":"Bacteriology Group, ICGEB, Padriciano 99, I-34149 Trieste, Italy"}]}],"member":"286","published-online":{"date-parts":[[2019,7,4]]},"reference":[{"key":"2022030905553930700_bib1","doi-asserted-by":"crossref","first-page":"1739","DOI":"10.1128\/AEM.69.3.1739-1747.2003","article-title":"Quorum-sensing system and stationary-phase sigma factor (rpoS) of the onion pathogen Burkholderia cepacia genomovar I type strain, ATCC 25416","volume":"69","author":"Aguilar","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"2022030905553930700_bib2","doi-asserted-by":"crossref","first-page":"824","DOI":"10.2144\/99265bm05","article-title":"The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knock-out and targeted DNA insertion into the chromosome of gram-negative 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