{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:39:10Z","timestamp":1762504750530,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,14]],"date-time":"2001-12-14T00:00:00Z","timestamp":1008288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004434","name":"Universit\u00e0 degli Studi di Firenze","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Relaxin (RLX) is a reproductive hormone with vasodilatatory properties on several organs, including the heart. RLX\u2010induced vasodilatation appears to depend on the stimulation of endogenous NO production. Here, we investigate whether RLX acts on rat coronary endothelial (RCE) cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    by inducing changes of NO generation and, if so, to clarify the possible mechanism of action. RCE cells were treated for 24 h with vehicle (controls) or RLX, alone or in association with inhibitors of NO synthesis or dexamethasone, which inhibits transcription of NO synthase gene. In some experiments, inactivated RLX was given in the place of authentic RLX. Expression of NO synthase isozymes II and III was analyzed by immunocytochemistry, Western blot, and RT\u2010PCR. NO production was evaluated by the Griess reaction for nitrite and the NO\u2010sensitive fluorophore DAF\u20102\/DA. Agonist\u2010induced changes of intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transient were studied with the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010sensitive fluorophore Fura 2\u2010AM. RLX was found to up regulate NOS II mRNA and protein and to stimulate intrinsic NO generation, likely through the activation of a dexamethasone\u2010sensitive transcription factor, and to decrease agonist\u2010induced intracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transient. Conversely, RLX had negligible effects on NOS III expression. By these biological effects, RLX may afford significant protection against cardiovascular disease.\n                  <\/jats:p>","DOI":"10.1096\/fj.01-0569fje","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T19:03:24Z","timestamp":1027710204000},"page":"1-19","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Relaxin up\u2010regulates inducible nitric oxide synthase expression and nitric oxide generation in rat coronary endothelial cells"],"prefix":"10.1096","volume":"16","author":[{"given":"Paola","family":"Failli","sequence":"first","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]},{"given":"Silvia","family":"Nistri","sequence":"additional","affiliation":[{"name":"Department of Anatomy Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]},{"given":"Silvia","family":"Quattrone","sequence":"additional","affiliation":[{"name":"Department of Anatomy Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]},{"given":"Luca","family":"Mazzetti","sequence":"additional","affiliation":[{"name":"Department of Preclinical and Clinical Pharmacology Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]},{"given":"Mario","family":"Bigazzi","sequence":"additional","affiliation":[{"name":"Prosperius Institute Florence Italy"}]},{"given":"Tatiana Bani","family":"Sacchi","sequence":"additional","affiliation":[{"name":"Department of Anatomy Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]},{"given":"Daniele","family":"Bani","sequence":"additional","affiliation":[{"name":"Department of Anatomy Histology and Forensic Medicine Section of Histology University of Florence Florence Italy"}]}],"member":"311","published-online":{"date-parts":[[2001,12,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-3623(96)00171-1"},{"key":"e_1_2_6_3_1","first-page":"296","article-title":"The local administration of relaxin induces changes in the microcirculation of the rat mesocaecum","volume":"112","author":"Bigazzi M.","year":"1986","journal-title":"Acta Endocrinol. 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