{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T13:28:25Z","timestamp":1768310905972,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2007,8,3]],"date-time":"2007-08-03T00:00:00Z","timestamp":1186099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003749","name":"L\u00e4nsstyrelsen \u00d6sterg\u00f6tland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003749","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:p>\n                    We studied whether the acute\u2010phase protein \u03b1\n                    <jats:sub>1<\/jats:sub>\n                    \u2010acid glycoprotein (AGP) induces rises in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in neutrophils and sought to identify the corresponding AGP receptor (or receptors). We found that AGP elicited a minimal rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in Fura\u20102\u2010loaded neutrophils, and this response was markedly enhanced by pretreatment with anti\n                    <jats:sc>\u2010L<\/jats:sc>\n                    \u2010selectin antibodies. (The EC\n                    <jats:sub>50<\/jats:sub>\n                    value of the AGP\u2010induced Ca\n                    <jats:sup>2+<\/jats:sup>\n                    response was 9 \u03bcg\/ml.) Activation of phospholipase\u2010C, Src tyrosine kinases, and PI3 kinases proved to be essential for the AGP\u2010mediated increase in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    , whereas the p38 MAPK and SYK signaling pathways were not involved. Furthermore, antibodies against sialic acid binding, immunoglobulin\u2010like lectin 5 (Siglec\u20105) and oligosac\u2010charide 3\u2032\u2010sialyl\u2010lactose both antagonized the AGP\u2010in\u2010duced response and caused an immediate increase in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    in anti\n                    <jats:sc>\u2010L<\/jats:sc>\n                    \u2010selectin\u2010treated neutrophils, which indicates a signaling capacity of Siglec\u20105. We used modified forms of AGP (treated with mild periodate or neuraminidase) to establish the importance of sialic acid residues. The modified forms of AGP caused a much smaller rise in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    than did unaltered AGP. Affinity chromatography confirmed that unchanged AGP, but not neuraminidase\u2010treated AGP, bound to Siglec\u20105. Our report provides the first evidence for a signaling capacity by AGP through a defined receptor. Pre\u2010engagement of\n                    <jats:sc>L<\/jats:sc>\n                    \u2010selectin significantly enhanced this signaling capacity.\u2014 Gunnarsson, P., Levander, L., P\u00e5hlsson, P., Greneg\u00e5rd, M. The acute\u2010phase protein \u03b11\u2010acid glycoprotein (AGP) induces rises in cytosolic Ca2+ in neutrophil granulocytes\n                    <jats:italic>via<\/jats:italic>\n                    sialic acid binding immunoglobulin\u2010like lectins (Siglecs).\n                    <jats:italic>FASEB J<\/jats:italic>\n                    . 21, 4059\u20134069 (2007)\n                  <\/jats:p>","DOI":"10.1096\/fj.07-8534com","type":"journal-article","created":{"date-parts":[[2007,8,3]],"date-time":"2007-08-03T21:28:42Z","timestamp":1186176522000},"page":"4059-4069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["The acute\u2010phase protein \u03b1\n                    <sub>1<\/sub>\n                    \u2010acid glycoprotein (AGP) induces rises in cytosolic Ca\n                    <sup>2+<\/sup>\n                    in neutrophil granulocytes\n                    <i>via<\/i>\n                    sialic acid binding immunoglobulin\u2010like lectins (Siglecs)"],"prefix":"10.1096","volume":"21","author":[{"given":"Peter","family":"Gunnarsson","sequence":"first","affiliation":[{"name":"Division of Pharmacology Department of Medicine and Care Division of Cell Biology Department of Biomedicine and Surgery Link\u00f6ping University Link\u00f6ping Sweden"}]},{"given":"Louise","family":"Levander","sequence":"additional","affiliation":[{"name":"Division of Cell Biology Department of Biomedicine and Surgery Link\u00f6ping University Link\u00f6ping Sweden"}]},{"given":"Peter","family":"P\u00e5hlsson","sequence":"additional","affiliation":[{"name":"Division of Cell Biology Department of Biomedicine and Surgery Link\u00f6ping University Link\u00f6ping Sweden"}]},{"given":"Magnus","family":"Greneg\u00e5rd","sequence":"additional","affiliation":[{"name":"Division of Pharmacology Department of Medicine and Care Division of Cell Biology Department of Biomedicine and Surgery Link\u00f6ping University Link\u00f6ping Sweden"}]}],"member":"311","published-online":{"date-parts":[[2007,8,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820137409061112"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00914025"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(94)90121-X"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915815"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/281677a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00917452"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(00)00153-9"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01049676"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780320319"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-0801(199811\/12)12:6<343::AID-BMC760>3.0.CO;2-6"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.6.2123"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M002788200"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"4021","DOI":"10.1016\/S0021-9258(17)41736-4","article-title":"Sulfatides trigger increase of cytosolic free calcium and enhanced expression of tumor necrosis factor\u2013alpha and interleukin\u20138 mRNA in human neutrophils. Evidence for a role of L\u2013selectin as a signaling molecule","volume":"269","author":"Laudanna C.","year":"1994","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)32335-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.25.15403"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.11.6841"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90377-4"},{"key":"e_1_2_6_19_1","first-page":"7","article-title":"Separation of leukocytes from blood and bone marrow","volume":"97","author":"Boyum A.","year":"1968","journal-title":"Introduction. Scand. J. Clin. Lab. Invest. Suppl."},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006983824890"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.4049\/jimmunol.154.5.2291","article-title":"Activation of human neutrophils through L\u2013selectin and Mac\u20131 molecules","volume":"154","author":"Crockett\u2013Torabi E.","year":"1995","journal-title":"J.Immunol."},{"key":"e_1_2_6_23_1","first-page":"H1302","article-title":"L\u2013selectin stimulation of canine neutrophil initiates calcium signal secondaryto tyrosine kinase activation","volume":"272","author":"Crockett\u2013Torabi E.","year":"1997","journal-title":"Am. J. Physiol."},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"6121","DOI":"10.1016\/S0021-9258(18)45546-9","article-title":"Nucleotide regulatory protein\u2013mediated activation of phospholipase C in human polymorphonuclear leukocytes is disrupted by phorbol esters","volume":"262","author":"Smith C. D.","year":"1987","journal-title":"J.Biol. Chem."},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2002.03808.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"crossref","first-page":"86258632","DOI":"10.1074\/jbc.275.12.8625","article-title":"New aspects of siglec binding specificities, including the significance of fucosylation and of the sialyl\u2013Tn epitope. Sialic acid\u2013binding immunoglobulin superfamily lectins","volume":"275","author":"Brinkman\u2013Van der Linden E. C.","year":"2000","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15376"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.273.5.G1031"},{"key":"e_1_2_6_29_1","first-page":"546","article-title":"Modification of the functional activity of neutrophils treated with acute phase response proteins","volume":"63","author":"Timoshenko A. V.","year":"1998","journal-title":"Biochemistry (Moscow)"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M906232199"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00336.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"3567","DOI":"10.1182\/blood.V86.9.3567.bloodjournal8693567","article-title":"Activation of Lyn is a common element of the stimulation of human neutrophils bysoluble and particulate agonists","volume":"86","author":"Gaudry M.","year":"1995","journal-title":"Blood"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.34.19969"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5455.1049"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5455.1046"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200202114"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.66.2.293"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.6.3874"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.290.5489.84"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.092601.111357"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(99)00024-3"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M502041200"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(03)00046-8"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-5800com"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.17.7538"},{"key":"e_1_2_6_46_1","first-page":"245","article-title":"Neutrophil adhesion to endothelial cells","volume":"19","author":"Abbassi O.","year":"1993","journal-title":"Blood Cells"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0105042"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.07-8534com","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.07-8534com","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.07-8534com","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:15:15Z","timestamp":1761675315000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.07-8534com"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,3]]},"references-count":46,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1096\/fj.07-8534com"],"URL":"https:\/\/doi.org\/10.1096\/fj.07-8534com","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,3]]},"assertion":[{"value":"2007-04-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-06-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}