{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:14:45Z","timestamp":1762298085205,"version":"3.37.3"},"reference-count":50,"publisher":"Microbiology Society","issue":"6","funder":[{"DOI":"10.13039\/100007802","name":"Institute for Fermentation, Osaka","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007802","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010157","name":"Noda Institute for Scientific Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010157","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology of Japan","doi-asserted-by":"crossref","award":["S1201003"],"award-info":[{"award-number":["S1201003"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:p>A temperature-sensitive mutation in<jats:italic>rplB,<\/jats:italic>designated<jats:italic>rplB142<\/jats:italic>, encodes a missense mutation at position 142 [His (CAT) to Leu (CTT)] of<jats:italic>Bacillus subtilis<\/jats:italic>ribosomal protein L2. The strain carrying the mutation grew more slowly than the wild-type, even at low temperatures, probably due to the formation of defective 70S ribosomes and the accumulation of incomplete 50S subunits (50S* subunits). Gel analysis indicated that amounts of L2 protein and also of L16 protein were reduced in ribosomes prepared from the<jats:italic>rplB142<\/jats:italic>mutant 90 min after increasing the growth temperature to 45 \u00b0C. These results suggest that the assembly of the L16 protein into the 50S subunit requires the native L2 protein. The H142L mutation in the defective L2 protein affected sporulation as well as growth, even at the permissive temperature. A suppressor mutation that restored both growth and sporulation of the<jats:italic>rplB142<\/jats:italic>mutant at low temperature was identified as a single base deletion located immediately upstream of the<jats:italic>yaaA<\/jats:italic>gene that resulted in an increase in its transcription. Furthermore, genetic analysis showed that enhanced synthesis of YaaA restores the functionality of L2 (H142L) by facilitating its assembly into 50S subunits.<\/jats:p>","DOI":"10.1099\/mic.0.076463-0","type":"journal-article","created":{"date-parts":[[2014,3,18]],"date-time":"2014-03-18T06:12:22Z","timestamp":1395123142000},"page":"1040-1053","source":"Crossref","is-referenced-by-count":12,"title":["Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2"],"prefix":"10.1099","volume":"160","author":[{"given":"Shota","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Life Science and Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Nishi-ikebukuro 3-34-1, Tokyo 171-8501, Japan"}]},{"given":"Osamu","family":"Tanigawa","sequence":"additional","affiliation":[{"name":"Department of Life Science and Research Center for Life 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