{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:53:28Z","timestamp":1762458808647,"version":"3.37.3"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2010,7,22]],"date-time":"2010-07-22T00:00:00Z","timestamp":1279756800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Swedish Science Council"},{"DOI":"10.13039\/501100001729","name":"Swedish Foundation for Strategic Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001729","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Magn. 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We show that DC performed some functions normally at 28\u00b0C, including phagocytosis and macropinocytosis. TLR-4 signaling via MAPK pathways was delayed at 28\u00b0C but reached normal levels, which may explain the observed slower kinetics of stimulated macropinocytosis and TNF production. TLR-4-induced NO production was compromised severely at 28\u00b0C. Collagen degradation and migration through matrigel-coated transwell inserts were decreased, but no effect on podosome number or DC migration through noncoated transwell filters was seen. Lowering the temperature differentially regulated functions associated with the role of DCs in adaptive immunity. LPS-induced up-regulation of CD86 was normal; however, CD40 up-regulation was suppressed after TLR-4 stimulation at 28\u00b0C. Nonactivated DC processed and presented antigen on MHC class II equally well at 28\u00b0C and 37\u00b0C. However, DCs that were loaded with antigens and stimulated with TLR ligand at 28\u00b0C were poor at activating T cells at 37\u00b0C compared with DCs that were activated and loaded with antigen at 37\u00b0C.<\/jats:p>","DOI":"10.1189\/jlb.0310155","type":"journal-article","created":{"date-parts":[[2010,7,23]],"date-time":"2010-07-23T03:51:02Z","timestamp":1279857062000},"page":"747-756","source":"Crossref","is-referenced-by-count":7,"title":["Dendritic cell function at low physiological temperature"],"prefix":"10.1093","volume":"88","author":[{"given":"Oscar","family":"Hammarfjord","sequence":"first","affiliation":[{"name":"Center for Infectious Medicine, F59, Department of Medicine, Karolinska Institutet, Karolinska University Hospital , Huddinge, Stockholm, Sweden"}]},{"given":"Robert P A","family":"Wallin","sequence":"additional","affiliation":[{"name":"Center for Infectious Medicine, F59, Department of Medicine, Karolinska Institutet, Karolinska University Hospital , Huddinge, Stockholm, Sweden"}]}],"member":"286","published-online":{"date-parts":[[2010,7,22]]},"reference":[{"key":"2023032712272973000_jlb0747-cit-b1","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1177\/1099800403260620","article-title":"Axillary and thoracic skin temperatures poorly comparable to core body temperature circadian rhythm: results from 2 adult populations","volume":"5","author":"Thomas","year":"2004","journal-title":"Biol. 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J. Immunol."},{"key":"2023032712272973000_jlb0747-cit-b22","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1172\/JCI113427","article-title":"Specific amino acid (L-arginine) requirement for the microbiostatic activity of murine macrophages","volume":"81","author":"Granger","year":"1988","journal-title":"J. Clin. Invest."},{"key":"2023032712272973000_jlb0747-cit-b23","doi-asserted-by":"crossref","first-page":"3577","DOI":"10.4049\/jimmunol.157.8.3577","article-title":"Induction of nitric oxide synthase in mouse dendritic cells by IFN-\u03b3, endotoxin, and interaction with allogeneic T cells: nitric oxide production is associated with dendritic cell apoptosis","volume":"157","author":"Lu","year":"1996","journal-title":"J. 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Cell Biol."},{"key":"2023032712272973000_jlb0747-cit-b36","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1084\/jem.182.2.389","article-title":"Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products","volume":"182","author":"Sallusto","year":"1995","journal-title":"J. Exp. Med."},{"key":"2023032712272973000_jlb0747-cit-b37","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/BF03403535","article-title":"Lipopolysaccharide signals activation of tumor necrosis factor biosynthesis through the ras\/raf-1\/MEK\/MAPK pathway","volume":"1","author":"Geppert","year":"1994","journal-title":"Mol. 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