{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T10:57:35Z","timestamp":1772017055491,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004530","name":"Universiti Putra Malaysia","doi-asserted-by":"publisher","award":["GPM\u20122018\/9660000, GPM\u20122019\/9678900, 2017\/9300436"],"award-info":[{"award-number":["GPM\u20122018\/9660000, GPM\u20122019\/9678900, 2017\/9300436"]}],"id":[{"id":"10.13039\/501100004530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Sultan Mizan Antarctic Research Foundation (YPASM)","award":["YPASM Smart Partnership Initiative (6300247)"],"award-info":[{"award-number":["YPASM Smart Partnership Initiative (6300247)"]}]},{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["British Antarctic Survey"],"award-info":[{"award-number":["British Antarctic Survey"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centro de Investigacion y Monitoreo Ambiental Ant\u00e0rctico","award":["CIMAA"],"award-info":[{"award-number":["CIMAA"]}]},{"DOI":"10.13039\/501100007140","name":"Jabatan Perkhidmatan Awam Malaysia","doi-asserted-by":"publisher","award":["personal scholarship"],"award-info":[{"award-number":["personal scholarship"]}],"id":[{"id":"10.13039\/501100007140","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biology"],"abstract":"<jats:p>Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent\u2019s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g\/L NH4Cl and 2.0% v\/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10\u201215 \u00b0C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g\/L NH4Cl, 12.5 \u00b0C and 1.75% v\/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, \u03bcmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h\u22121, 1.356% v\/v and 1.238% v\/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils.<\/jats:p>","DOI":"10.3390\/biology10060493","type":"journal-article","created":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T10:38:39Z","timestamp":1622630319000},"page":"493","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica"],"prefix":"10.3390","volume":"10","author":[{"given":"Ahmad Fareez Ahmad","family":"Roslee","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9060-7727","authenticated-orcid":false,"given":"Claudio","family":"Gomez-Fuentes","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Universidad de Magallanes, Avda. Bulnes, Punta Arenas 01855, Chile"},{"name":"Center for Research and Antarctic Environmental Monitoring (CIMAA), Universidad de Magallanes, Avda. Bulnes, Punta Arenas 01855, Chile"}]},{"given":"Nur Nadhirah","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"}]},{"given":"Nor Azmi","family":"Shaharuddin","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"}]},{"given":"Azham","family":"Zulkharnain","sequence":"additional","affiliation":[{"name":"Department of Bioscience and Engineering, Shibaura Institute of Technology, College of Systems Engineering and Science, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan"}]},{"given":"Khalilah","family":"Abdul Khalil","sequence":"additional","affiliation":[{"name":"Faculty of Applied Sciences, School of Biology, Universiti Teknologi MARA, Shah Alam 40450, Malaysia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8497-9903","authenticated-orcid":false,"given":"Peter","family":"Convey","sequence":"additional","affiliation":[{"name":"British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 0ET, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7625-3704","authenticated-orcid":false,"given":"Siti Aqlima","family":"Ahmad","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Malaysia"},{"name":"Center for Research and Antarctic Environmental Monitoring (CIMAA), Universidad de Magallanes, Avda. Bulnes, Punta Arenas 01855, Chile"},{"name":"National Antarctic Research Centre, B303 Level 3, Block B, IPS Building, Universiti Malaya, Kuala Lumpur 50603, Malaysia"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.envpol.2016.02.035","article-title":"Tracking human footprints in Antarctica through passive sampling of polycyclic aromatic hydrocarbons in inland lakes","volume":"213","author":"Yao","year":"2016","journal-title":"Environ. Pollut."},{"key":"ref_2","unstructured":"IAATO (2019, January 27\u201328). IAATO overview of Antarctic tourism: 2018\u201319 season and preliminary estimates for 2019\u201320 Season. Information Paper 140. Proceedings of the Antarctic Treaty Consultative Meeting XLII, Prague, Czech Republic."},{"key":"ref_3","first-page":"77","article-title":"Mini review on phenol biodegradation in Antarctica using native microorganisms","volume":"28","author":"Subramaniam","year":"2020","journal-title":"Asia Pac. J. Mol. Biol. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1017\/S0954102009001722","article-title":"Impacts of local human activities on the Antarctic environment: A review","volume":"21","author":"Tin","year":"2009","journal-title":"Antarct. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1023\/A:1005766302869","article-title":"The fate in the marine environment of a minor diesel fuel spill from Antarctic research station","volume":"46","author":"Cripps","year":"1997","journal-title":"Environ. Monit. Assess."},{"key":"ref_6","first-page":"248","article-title":"Bioremediation in Antarctic Soils","volume":"6","author":"Peixoto","year":"2015","journal-title":"J. Pet. Environ. Biotechnol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.emcon.2019.04.002","article-title":"Sources and environmental fate of pyrogenic polycyclic aromatic hydrocarbons (PAHs) in the Arctic","volume":"5","author":"Balmer","year":"2019","journal-title":"Emerg. Contam."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.jenvman.2018.07.092","article-title":"Modelling of vapour intrusion into a building impacted by a fuel spill in Antarctica","volume":"23","author":"McWatters","year":"2019","journal-title":"J. Environ. Manag."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.24275\/rmiq\/Bio1072","article-title":"Kinetic studies of marine psychrotolerant microorganisms capable of degrading diesel in the presence of heavy metals","volume":"19","author":"Zakaria","year":"2020","journal-title":"Rev. Mex. Ing. Qu\u00edm."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ejpe.2015.03.011","article-title":"A review on polycyclic aromatic hydrocarbons: Source, environmental impact, effect on human health and remediation","volume":"25","author":"Mansour","year":"2016","journal-title":"Egypt. J. Pet."},{"key":"ref_11","unstructured":"Dupuis, A., and Ucan-Marin, F. (2015). A Literature Review on the Aquatic Toxicology of Petroleum Oil: An Overview of Oil Properties and Effects to Aquatic Biota, DFO Canadian Science Advisory Secretariat."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.marpolbul.2016.02.047","article-title":"Persistent organic pollutants and polycyclic aromatic hydrocarbons in penguins of the genus Pygoscelis in Admiralty Bay\u2013An Antarctic specially managed area","volume":"106","author":"Montone","year":"2015","journal-title":"Mar. Pollut. Bull."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.marpolbul.2016.04.023","article-title":"A comprehensive guide of remediation technologies for oil contaminated soil\u2014Present works and future directions","volume":"109","author":"Lim","year":"2016","journal-title":"Mar. Pollut. Bull."},{"key":"ref_14","first-page":"974","article-title":"Bioremediation: A sustainable tool for environmental management\u2014A review","volume":"3","author":"Bhatnagar","year":"2013","journal-title":"Annu. Rev. Res. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s00792-019-01153-0","article-title":"Statistical optimisation of growth conditions and diesel degradation by the Antarctic bacterium, Rhodococcus sp. strain AQ5\u201207","volume":"24","author":"Roslee","year":"2020","journal-title":"Extremophiles"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Ibrahim, S., Abdul Khalil, K., Zahri, K.N.M., Gomez-Fuentes, C., Convey, P., Zulkharnain, A., Sabri, S., Alias, S.A., Gonzalez-Rocha, G., and Ahmad, S.A. (2020). Biosurfactant production and growth kinetics studies of the waste canola oil-degrading bacterium Rhodococcus erythropolis AQ5-07 from Antarctica. Molecules, 25.","DOI":"10.3390\/molecules25173878"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1007\/s00792-005-0498-4","article-title":"Bioremediation of hydrocarbon contaminated Polar soils","volume":"10","author":"Aislabie","year":"2006","journal-title":"Extremophiles"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1016\/j.biortech.2017.02.064","article-title":"Impact of electro-stimulation on denitrifying bacterial growth and analysis of bacterial growth kinetics using a modified Gompertz model in a bio-electrochemical denitrification reactor","volume":"232","author":"Liu","year":"2017","journal-title":"Bioresour. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1016\/j.procbio.2004.06.042","article-title":"Interfacial effects in a two-phase partitioning bioreactor: Degradation of polycyclic aromatic hydrocarbons (PAHs) by a hydrophobic mycobacterium","volume":"40","author":"MacLeod","year":"2005","journal-title":"Process. Biochem."},{"key":"ref_20","first-page":"9","article-title":"Bioremediation of oil spills: A review of challenges for research advancement","volume":"8","author":"Macaulay","year":"2014","journal-title":"Ann. Environ. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1016\/j.jfoodeng.2005.11.024","article-title":"Modeling and optimization I: Usability of response surface methodology","volume":"78","author":"Bas","year":"2007","journal-title":"J. Food Eng."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Abdulrasheed, M., Zulkharnain, A., Zakaria, N.N., Roslee, A.F.A., Khalil, K.A., Napis, S., Convey, P., Gomez\u2012Fuentes, C., and Ahmad, S.A. (2020). Response surface methodology optimisation and kinetics of diesel degradation by cold\u2012adapted Antarctic bacterium, Arthrobacter sp. Strain AQ5\u201205. Sustainability, 12.","DOI":"10.3390\/su12176966"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.22438\/jeb\/41\/6\/MRN-1496","article-title":"Statistical optimisation for enhancement of phenol biodegradation by the Antarctic soil bacterium Arthrobacter sp. strain AQ5-15 using response surface methodology","volume":"41","author":"Subramaniam","year":"2020","journal-title":"J. Environ. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.watres.2015.08.010","article-title":"Physiological adaptation of growth kinetics in activated sludge","volume":"85","author":"Friedrich","year":"2015","journal-title":"Water Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1007\/s00449-013-1089-x","article-title":"Kinetics study of pyridine biodegradation by a novel bacterial strain, Rhizobium sp. NJUST18","volume":"37","author":"Shen","year":"2014","journal-title":"Bioprocess Biosyst. Eng."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Pla, M.L., Oltra, S., Esteban, M.D., Andreu, S., and Palop, A. (2015). Comparison of primary models to predict microbial growth by the plate count and absorbance methods. Biomed Res. Int., 365025.","DOI":"10.1155\/2015\/365025"},{"key":"ref_27","unstructured":"ASTM D421-85 (2007). Standard Practice for Dry Preparation of Soil Samples for Particle-Size Analysis and Determination of Soil Constants (withdrawn 2016), ASTM International."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s11270-008-9704-1","article-title":"An improved gravimetric method to determine total petroleum hydrocarbons in contaminated soils","volume":"194","author":"Villalobos","year":"2008","journal-title":"Water Air Soil Pollut."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1128\/jb.41.5.653-673.1941","article-title":"The utilisation of certain hydrocarbons by microorganisms","volume":"41","author":"Bushnell","year":"1941","journal-title":"J. Bacteriol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s11270-017-3368-7","article-title":"Assessing the potential of organic solvents on total petroleum hydrocarbon extraction from diesel-contaminated soils","volume":"228","author":"Kwon","year":"2017","journal-title":"Water Air Soil Pollut."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1093\/biomet\/33.4.305","article-title":"The design of optimum multifactorial experiments","volume":"33","author":"Plackett","year":"1946","journal-title":"Biometrika"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.2517-6161.1951.tb00067.x","article-title":"On the experimental attainment of optimum condition","volume":"13","author":"Box","year":"1951","journal-title":"J. R. Stat. Soc."},{"key":"ref_33","first-page":"209","article-title":"Growth of bacterial populations and the available substrate concentration","volume":"3208","author":"Tessier","year":"1942","journal-title":"Rev. Scientif. Instrum."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1146\/annurev.mi.03.100149.002103","article-title":"The growth of bacterial cultures","volume":"3","author":"Monod","year":"1949","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_35","unstructured":"Plimmer, R.H.A., and Hopkins, F.G. (1930). Enzymes. Monographs and Biochemistry, Longmans and Green."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1002\/(SICI)1097-0290(20000320)67:6<671::AID-BIT6>3.0.CO;2-W","article-title":"Kinetics of product inhibition in alcohol fermentation","volume":"67","author":"Aiba","year":"1968","journal-title":"J. Biotechnol. Bioeng."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1002\/bit.260120504","article-title":"The influence of high substrate concentrations on microbial kinetics","volume":"12","author":"Edwards","year":"1970","journal-title":"Biotechnol. Bioeng."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"97","DOI":"10.2323\/jgam.19.97","article-title":"Dynamic behavior of the chemostat subject to product inhibition","volume":"19","author":"Yano","year":"1973","journal-title":"J. Gen. Appl. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/bit.260290215","article-title":"Generalization of Monod kinetics for analysis of growth data with substrate inhibition","volume":"29","author":"Luong","year":"1987","journal-title":"Biotechnol. Bioeng."},{"key":"ref_40","unstructured":"Anderson, M.J., and Whitcomb, P.J. (2016). RSM Simplified: Optimizing Processes Using Response Surface Methods for Design of Experiments, Taylor Francis Group, LLC. [2nd ed.]."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1007\/s00300-017-2216-y","article-title":"Biodegradation of phenol by cold-adapted bacteria from Antarctic soils","volume":"41","author":"Lee","year":"2018","journal-title":"Polar Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1128\/mr.59.2.201-222.1995","article-title":"Mechanisms of membrane toxicity of hydrocarbons","volume":"59","author":"Sikkema","year":"1995","journal-title":"Microbiol. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3034","DOI":"10.1128\/AEM.03414-13","article-title":"Soil microbial responses to increased moisture and organic resources along a salinity gradient in a polar desert","volume":"80","author":"Okie","year":"2014","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2012","DOI":"10.1128\/AEM.04052-15","article-title":"Comparative toxicities of salts on microbial processes in soil","volume":"82","author":"Rath","year":"2016","journal-title":"Appl. Environ. Microb."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.polar.2010.05.010","article-title":"Metagenomic analyses of the dominant bacterial community in the Fildes Peninsula, King George Island (South Shetland Islands)","volume":"4","author":"Foong","year":"2010","journal-title":"Polar Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"966","DOI":"10.22438\/jeb\/41\/5\/MRN-1319","article-title":"Effects of heavy metals on diesel metabolism of psychrotolerant strains of Arthrobacter sp. from Antarct","volume":"41","author":"Abdulrasheed","year":"2020","journal-title":"J. Environ. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1111\/j.1758-2229.2011.00266.x","article-title":"Hypolithic communities: Important nitrogen sources in Antarctic desert soil","volume":"3","author":"Cowan","year":"2011","journal-title":"Environ. Microb. Rep."},{"key":"ref_48","first-page":"191","article-title":"Bioremediation of hydrocarbon-contaminated soils in cold regions: Development of a pre-optimized biostimulation biopile\u2013scale field assay in Antarctica","volume":"591","author":"Alvarez","year":"2017","journal-title":"Sci. Total Environ."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.coldregions.2006.07.001","article-title":"Nitrogen requirements for maximizing petroleum bioremediation in a sub-Antarctic soil","volume":"48","author":"Walworth","year":"2007","journal-title":"Cold Reg. Sci. Technol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1016\/j.cub.2019.04.038","article-title":"Nitrogen inputs by marine vertebrates drive abundance and richness in Antarctic terrestrial ecosystems","volume":"29","author":"Bokhorst","year":"2019","journal-title":"Curr. Biol."},{"key":"ref_51","first-page":"294","article-title":"Nutrient abundance and variability from soils in the coast of King George Island","volume":"18","author":"Lachacz","year":"2018","journal-title":"J. Soil Sci. Plant Nutr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.ibiod.2012.05.004","article-title":"Low-temperature biodegradation of petroleum hydrocarbons (n-alkanes, phenol, anthracene, pyrene) by four actinobacterial strains","volume":"84","author":"Margesin","year":"2013","journal-title":"Int. Biodeterior. Biodegrad."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1007\/s00300-018-2299-0","article-title":"The importance of understanding annual and shorter-term temperature patterns and variation in the surface levels of polar soils for terrestrial biota","volume":"41","author":"Convey","year":"2018","journal-title":"Polar Biol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Ahmed, S.A., Abdella, M.A.A., El-Sherbiny, G.M., Ibrahim, A.M., El-Shamy, A.R., and Atalla, S.M.M. (2019). Application of one \u2013factor- at-a-time and statistical designs to enhance \u03b1-amylase production by a newly isolate Bacillus subtilis strain-MK1. Biocatal. Agric. Biotehnol.","DOI":"10.1016\/j.bcab.2019.101397"},{"key":"ref_55","first-page":"501","article-title":"Indices for performance evaluation of predictive models in food microbiology","volume":"81","author":"Ross","year":"1996","journal-title":"J. Appl. Bacteriol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.ijfoodmicro.2004.03.026","article-title":"Statistical evaluation of mathematical models for microbial growth","volume":"96","author":"Lopez","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0167-7012(00)00219-0","article-title":"Comparison of maximum specific growth rates and lag times estimated from absorbance and viable count data by different mathematical models","volume":"43","author":"Dalgaard","year":"2001","journal-title":"J. Microbiol. Methods"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1007\/s12210-014-0344-7","article-title":"Kinetics of diesel degradation by an acrylamide-degrading bacterium","volume":"25","author":"Ahmad","year":"2014","journal-title":"Rend. Lincei"}],"container-title":["Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-7737\/10\/6\/493\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:10:01Z","timestamp":1760163001000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-7737\/10\/6\/493"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":58,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["biology10060493"],"URL":"https:\/\/doi.org\/10.3390\/biology10060493","relation":{},"ISSN":["2079-7737"],"issn-type":[{"value":"2079-7737","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}