{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:59:07Z","timestamp":1772895547543,"version":"3.50.1"},"reference-count":46,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T00:00:00Z","timestamp":1624665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103639"],"award-info":[{"award-number":["P20GM103639"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008937","name":"OU College of Medicine Alumni Association","doi-asserted-by":"publisher","award":["C5112501"],"award-info":[{"award-number":["C5112501"]}],"id":[{"id":"10.13039\/100008937","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Centromere Protein I (CENP-I) is a member of the CENP-H\/I\/K complex. CENP-H\/I\/K is a major component of the inner kinetochore and aids in ensuring proper chromosomal segregation during mitosis. In addition to this chromosomal segregation function, CENP-I also plays a role in DNA double-strand break (DSB) repair. Loss of CENP-I leads to increased endogenous 53BP1 foci and R-loop formation, while reducing cellular survival after ionizing radiation and Niraparib, a PARP1 small molecule inhibitor, exposures. Cells lacking CENP-I display delayed 53BP1 foci regression, an indication that DSB repair is impaired. Additionally, loss of CENP-I impairs the homologous recombination DSB repair pathway, while having no effect on the non-homologous end-joining pathway. Interestingly, we find that RNaseH1 expression restores HR capacity in CENP-I deficient cells. Importantly, CENP-I expression is elevated in glioma tissue as compared to normal brain tissue. This elevated expression also correlates with poor overall patient survival. These data highlight the multi-functional role CENP-I plays in maintaining genetic, as well as chromosomal, stability and tumor survival.<\/jats:p>","DOI":"10.3390\/cancers13133202","type":"journal-article","created":{"date-parts":[[2021,6,27]],"date-time":"2021-06-27T22:24:57Z","timestamp":1624832697000},"page":"3202","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Loss of CENP-I Impairs Homologous Recombination and Sensitizes Cells to PARP1 Inhibition"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4836-8819","authenticated-orcid":false,"given":"Tuyen T.","family":"Dang","sequence":"first","affiliation":[{"name":"Stephenson Cancer Center, Department of Neurosurgery, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, USA"}]},{"given":"Julio C.","family":"Morales","sequence":"additional","affiliation":[{"name":"Stephenson Cancer Center, Department of Neurosurgery, University of Oklahoma Health Science Center, Oklahoma City, OK 73104, USA"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1016\/j.cell.2018.08.027","article-title":"The Multifaceted Role of Chromosomal Instability in Cancer and Its Microenvironment","volume":"174","author":"Bakhoum","year":"2018","journal-title":"Cell"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"450","DOI":"10.2307\/3572025","article-title":"X-ray sensitivity during the cell generation cycle of cultured Chinese hamster cells","volume":"29","author":"Sinclair","year":"1966","journal-title":"Radiat. 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