{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:14:43Z","timestamp":1768029283808,"version":"3.49.0"},"reference-count":45,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000830","name":"North Atlantic Treaty Organization","doi-asserted-by":"publisher","award":["SfP984637"],"award-info":[{"award-number":["SfP984637"]}],"id":[{"id":"10.13039\/501100000830","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003549","name":"Orsz\u00e1gos Tudom\u00e1nyos Kutat\u00e1si Alapprogramok","doi-asserted-by":"publisher","award":["K109865"],"award-info":[{"award-number":["K109865"]}],"id":[{"id":"10.13039\/501100003549","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxins"],"abstract":"<jats:p>Levels of mycotoxins produced by Fusarium species in genetically modified (GM) and near-isogenic maize, were determined using multi-analyte, microbead-based flow immunocytometry with fluorescence detection, for the parallel quantitative determination of fumonisin B1, deoxynivalenol, zearalenone, T-2, ochratoxin A, and aflatoxin B1. Maize varieties included the genetic events MON 810 and DAS-59122-7, and their isogenic counterparts. Cobs were artificially infested by F. verticillioides and F. proliferatum conidia, and contained F. graminearum and F. sporotrichoides natural infestation. The production of fumonisin B1 and deoxynivalenol was substantially affected in GM maize lines: F. verticillioides, with the addition of F. graminearum and F. sporotrichoides, produced significantly lower levels of fumonisin B1 (~300 mg\u00b7kg\u22121) in DAS-59122-7 than in its isogenic line (~580 mg\u00b7kg\u22121), while F. proliferatum, in addition to F. graminearum and F. sporotrichoides, produced significantly higher levels of deoxynivalenol (~18 mg\u00b7kg\u22121) in MON 810 than in its isogenic line (~5 mg\u00b7kg\u22121). Fusarium verticillioides, with F. graminearum and F. sporotrichoides, produced lower amounts of deoxynivalenol and zearalenone than F. proliferatum, with F. graminearum and F. sporotrichoides. T-2 toxin production remained unchanged when considering the maize variety. The results demonstrate the utility of the Fungi-Plex\u2122 quantitative flow immunocytometry method, applied for the high throughput parallel determination of the target mycotoxins.<\/jats:p>","DOI":"10.3390\/toxins9020070","type":"journal-article","created":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T11:36:58Z","timestamp":1487763418000},"page":"70","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Determination of Mycotoxin Production of Fusarium Species in Genetically Modified Maize Varieties by Quantitative Flow Immunocytometry"],"prefix":"10.3390","volume":"9","author":[{"given":"Hajnalka","family":"B\u00e1n\u00e1ti","sequence":"first","affiliation":[{"name":"Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ott\u00f3 \u00fat 15, H-1022 Budapest, Hungary"}]},{"given":"B\u00e9la","family":"Darvas","sequence":"additional","affiliation":[{"name":"Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ott\u00f3 \u00fat 15, H-1022 Budapest, Hungary"}]},{"given":"Szilvia","family":"Feh\u00e9r-T\u00f3th","sequence":"additional","affiliation":[{"name":"Soft Flow Hungary R&amp;D Ltd., \u00dcr\u00f6gi fasor 2\/A, H-7634 P\u00e9cs, Hungary"}]},{"given":"\u00c1rp\u00e1d","family":"Cz\u00e9h","sequence":"additional","affiliation":[{"name":"Soft Flow Hungary R&amp;D Ltd., \u00dcr\u00f6gi fasor 2\/A, H-7634 P\u00e9cs, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5816-3775","authenticated-orcid":false,"given":"Andr\u00e1s","family":"Sz\u00e9k\u00e1cs","sequence":"additional","affiliation":[{"name":"Agro-Environmental Research Institute, National Research and Innovation Centre, Herman Ott\u00f3 \u00fat 15, H-1022 Budapest, Hungary"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1023\/A:1026033021542","article-title":"Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops","volume":"109","author":"Logrieco","year":"2003","journal-title":"Eur. 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