{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:35:06Z","timestamp":1775673306968,"version":"3.50.1"},"reference-count":27,"publisher":"Elsevier BV","issue":"16","license":[{"start":{"date-parts":[[1987,6,1]],"date-time":"1987-06-01T00:00:00Z","timestamp":549504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[1987,6,1]],"date-time":"1987-06-01T00:00:00Z","timestamp":549504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T00:00:00Z","timestamp":1602806400000},"content-version":"vor","delay-in-days":12191,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["jbc.org","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Journal of Biological Chemistry"],"published-print":{"date-parts":[[1987,6]]},"DOI":"10.1016\/s0021-9258(18)47646-6","type":"journal-article","created":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T05:23:46Z","timestamp":1609824226000},"page":"7859-7864","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":276,"title":["Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli."],"prefix":"10.1016","volume":"262","author":[{"given":"H","family":"Ikemura","sequence":"first","affiliation":[]},{"given":"H","family":"Takagi","sequence":"additional","affiliation":[]},{"given":"M","family":"Inouye","sequence":"additional","affiliation":[]}],"member":"78","reference":[{"key":"10.1016\/S0021-9258(18)47646-6_bib1","series-title":"The Enzymes","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/S1874-6047(08)60407-2","volume":"3","author":"Markland","year":"1971"},{"key":"10.1016\/S0021-9258(18)47646-6_bib2","series-title":"The Enzymes","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S1874-6047(08)60406-0","volume":"3","author":"Kraut","year":"1971"},{"key":"10.1016\/S0021-9258(18)47646-6_bib3","doi-asserted-by":"crossref","first-page":"3096","DOI":"10.1073\/pnas.83.10.3096","volume":"83","author":"Powers","year":"1986","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0021-9258(18)47646-6_bib4","doi-asserted-by":"crossref","first-page":"3743","DOI":"10.1073\/pnas.83.11.3743","volume":"83","author":"Bryan","year":"1983","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/S0021-9258(18)47646-6_bib5","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0378-1119(85)90140-4","volume":"34","author":"Wells","year":"1985","journal-title":"Gene (Amst.)"},{"key":"10.1016\/S0021-9258(18)47646-6_bib6","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1038\/318375a0","volume":"318","author":"Thomas","year":"1985","journal-title":"Nature"},{"key":"10.1016\/S0021-9258(18)47646-6_bib7","doi-asserted-by":"crossref","first-page":"6518","DOI":"10.1016\/S0021-9258(18)88811-1","volume":"260","author":"Estell","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib8","doi-asserted-by":"crossref","first-page":"6564","DOI":"10.1016\/S0021-9258(19)84599-4","volume":"261","author":"Wells","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib9","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1128\/jb.158.2.411-418.1984","volume":"158","author":"Stahl","year":"1984","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0021-9258(18)47646-6_bib10","doi-asserted-by":"crossref","first-page":"10176","DOI":"10.1016\/S0021-9258(18)67507-6","volume":"261","author":"Wong","year":"1986","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib11","doi-asserted-by":"crossref","first-page":"7911","DOI":"10.1093\/nar\/11.22.7911","volume":"11","author":"Wells","year":"1983","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0021-9258(18)47646-6_bib12","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1128\/jb.159.3.811-819.1984","volume":"159","author":"Vasantha","year":"1984","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0021-9258(18)47646-6_bib13","doi-asserted-by":"crossref","first-page":"8913","DOI":"10.1093\/nar\/13.24.8913","volume":"13","author":"Jacobs","year":"1985","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0021-9258(18)47646-6_bib14","doi-asserted-by":"crossref","first-page":"4340","DOI":"10.1016\/S0021-9258(18)34727-6","volume":"257","author":"Chang","year":"1982","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib15","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/0378-1119(83)90102-6","volume":"22","author":"Shortle","year":"1983","journal-title":"Gene (Amst.)"},{"key":"10.1016\/S0021-9258(18)47646-6_bib16","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1128\/jb.160.1.15-21.1984","volume":"160","author":"Yang","year":"1984","journal-title":"J. Bacteriol."},{"key":"10.1016\/S0021-9258(18)47646-6_bib17","doi-asserted-by":"crossref","first-page":"2670","DOI":"10.1016\/S0021-9258(18)89413-3","volume":"260","author":"Takahara","year":"1985","journal-title":"J. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib18","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1016\/S0022-2836(61)80047-8","volume":"3","author":"Marmur","year":"1961","journal-title":"J. Mol. Biol."},{"key":"10.1016\/S0021-9258(18)47646-6_bib19","first-page":"289","volume":"1","author":"Nakamura","year":"1982","journal-title":"J. Mol. Appl. Genet."},{"key":"10.1016\/S0021-9258(18)47646-6_bib20","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1002\/j.1460-2075.1984.tb02151.x","volume":"3","author":"Ghrayeb","year":"1984","journal-title":"EMBO J."},{"key":"10.1016\/S0021-9258(18)47646-6_bib21","first-page":"160","volume":"36","author":"Murao","year":"1972","journal-title":"Agric. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib22","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1080\/00021369.1973.10860791","volume":"37","author":"Sato","year":"1973","journal-title":"Agric. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib23","first-page":"2227","volume":"38","author":"Sato","year":"1974","journal-title":"Agric. Biol. Chem."},{"key":"10.1016\/S0021-9258(18)47646-6_bib24","doi-asserted-by":"crossref","first-page":"4539","DOI":"10.1093\/nar\/12.11.4539","volume":"12","author":"Sinha","year":"1984","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/S0021-9258(18)47646-6_bib25","series-title":"Synthesis of DNA, RNA, and Their Application","author":"Inouye","year":"1987"},{"key":"10.1016\/S0021-9258(18)47646-6_bib26","series-title":"Experiments in Molecular Genetics","first-page":"431","author":"Miller","year":"1972"},{"key":"10.1016\/S0021-9258(18)47646-6_bib27","doi-asserted-by":"crossref","first-page":"1401","DOI":"10.1128\/jb.133.3.1401-1411.1978","volume":"133","author":"Strongin","year":"1978","journal-title":"J. Bacteriol."}],"container-title":["Journal of Biological Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925818476466?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0021925818476466?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T05:38:25Z","timestamp":1758173905000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0021925818476466"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,6]]},"references-count":27,"journal-issue":{"issue":"16","published-print":{"date-parts":[[1987,6]]}},"alternative-id":["S0021925818476466"],"URL":"https:\/\/doi.org\/10.1016\/s0021-9258(18)47646-6","relation":{},"ISSN":["0021-9258"],"issn-type":[{"value":"0021-9258","type":"print"}],"subject":[],"published":{"date-parts":[[1987,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Requirement of pro-sequence for the production of active subtilisin E in Escherichia coli.","name":"articletitle","label":"Article Title"},{"value":"Journal of Biological Chemistry","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/S0021-9258(18)47646-6","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 1987 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.","name":"copyright","label":"Copyright"}]}}