{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T22:24:28Z","timestamp":1757543068747},"reference-count":0,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1989,4,1]],"date-time":"1989-04-01T00:00:00Z","timestamp":607392000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[1989,4]]},"abstract":"<jats:p>The colonization factor antigen I (CFA\/I) gene has been isolated and sequenced. The amino acid sequence of CFA\/I deduced from the nucleotide sequence is composed of 170 amino acids. The first 23 amino acids are considered to be the signal peptide of the CFA\/I protein since they are not present in the protein sequence. Among the remaining amino acids, only two are different from the protein sequence: amino acid position 76 is an aspartic acid instead of an asparagine, and position 97 is a serine instead of an alanine. The CFA\/I gene has a typical Shine-Dalgarno sequence located 10 base pairs (bp) upstream from the initiation codon. The sequence TACAAT located 48 bp upstream from the initiation codon was tentatively designated the -10 sequence of the CFA\/I gene promoter. No sequences homologous to the consensus -35 promoter sequence was found. A pair of inverted repeat sequences followed by a stretch of eight A's are located 45 bp downstream from the termination codon of the CFA\/I gene; this region may be a rho-independent transcriptional terminator.<\/jats:p>","DOI":"10.1128\/iai.57.4.1126-1130.1989","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T13:10:34Z","timestamp":1578057034000},"page":"1126-1130","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli"],"prefix":"10.1128","volume":"57","author":[{"given":"T K","family":"Karjalainen","sequence":"first","affiliation":[{"name":"Department of Pathology, Indiana University School of Medicine, Indianapolis 46223."}]},{"given":"D G","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Pathology, Indiana University School of Medicine, Indianapolis 46223."}]},{"given":"M","family":"So","sequence":"additional","affiliation":[{"name":"Department of Pathology, Indiana University School of Medicine, Indianapolis 46223."}]},{"given":"C H","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology, Indiana University School of Medicine, Indianapolis 46223."}]}],"member":"235","container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.57.4.1126-1130.1989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.57.4.1126-1130.1989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T23:02:13Z","timestamp":1646434933000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.57.4.1126-1130.1989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,4]]}},"alternative-id":["10.1128\/iai.57.4.1126-1130.1989"],"URL":"https:\/\/doi.org\/10.1128\/iai.57.4.1126-1130.1989","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,4]]},"assertion":[{"value":"1989-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}