{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T19:24:42Z","timestamp":1793647482157,"version":"4.5.1"},"reference-count":58,"publisher":"American Medical Association (AMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAMA Netw Open"],"abstract":"Importance<\/jats:title>Congenital heart disease (CHD) is the most common human organ malformation, affecting approximately 1 of 125 newborns globally.<\/jats:p><\/jats:sec>Objectives<\/jats:title>Assessing the performance of 2 diagnostic tests using minimal amounts of dried blood spots (DBS) to identify high-risk CHD compared with controls in a Swedish cohort of neonates.<\/jats:p><\/jats:sec>Design, Setting, and Participants<\/jats:title>This diagnostic study took place in Sweden between 2019 and 2023 and enrolled full-term babies born between 2005 and 2023. All cases were identified through centralized pediatric cardiothoracic surgical services in Lund and Gothenburg, Sweden. Controls were followed up for 1 year to ensure no late presentations of high-risk CHD occurred. Cases were verified through surgical records and echocardiography.<\/jats:p><\/jats:sec>Exposure<\/jats:title>High-risk CHD, defined as cases requiring cardiac surgical management during infancy due to evolving signs of heart failure or types in which the postnatal circulation depends on patency of the arterial duct. Using 3-\u03bcL DBS samples, automated quantitative tests for NT-proBNP and interleukin 1 receptor-like 1 (IL-1 RL1; formerly known as soluble ST2) were compared against established CHD screening methods.<\/jats:p><\/jats:sec>Main Outcomes and Measures<\/jats:title>Performance of DBS tests to detect high-risk CHD using receiver operating characteristic curves; Bland-Altman and Pearson correlation analyses to compare IL-1 RL1 DBS with plasma blood levels.<\/jats:p><\/jats:sec>Results<\/jats:title>A total of 313 newborns were included (mean [SD] gestational age, 39.4 [1.3] weeks; 181 [57.8%] male). Mean (SD) birthweight was 3495 (483) grams. Analyzed DBS samples included 217 CHD cases and 96 controls. Among the CHD cases, 188 participants (89.3%) were high-risk types, of which 73 (38.8%) were suspected prenatally. Of the 188 high-risk cases, 94 (50.0%) passed pulse oximetry screening and 36 (19.1%) were initially discharged after birth without diagnoses. Combining NT-proBNP and IL-1 RL1 tests performed well in comparison with existing screening methods and enabled additional identification of asymptomatic babies with receiver operating characteristic area under the curve 0.95 (95% CI, 0.93-0.98).<\/jats:p><\/jats:sec>Conclusions and relevance<\/jats:title>In this diagnostic study, NT-proBNP and IL-1 RL1 DBS assays identified high-risk CHD in a timely manner, including in asymptomatic newborns, and improved overall screening performance in this cohort from Sweden. Prospective evaluation of this novel approach is warranted.<\/jats:p><\/jats:sec>","DOI":"10.1001\/jamanetworkopen.2024.18097","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T15:03:24Z","timestamp":1719241404000},"page":"e2418097","source":"Crossref","is-referenced-by-count":9,"title":["Newborn Screening for High-Risk Congenital Heart Disease by Dried Blood Spot Biomarker Analysis"],"prefix":"10.1001","volume":"7","author":[{"given":"Henning","family":"Clausen","sequence":"first","affiliation":[{"name":"Medical Faculty, Lund University, Sweden"},{"name":"Children\u2019s Heart Centre, Skane\u2019s University Hospital, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elin","family":"Friberg","sequence":"additional","affiliation":[{"name":"Medical Faculty, Lund University, Sweden"},{"name":"Children\u2019s Heart Centre, Skane\u2019s University Hospital, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Lannering","sequence":"additional","affiliation":[{"name":"Medical Faculty, Gothenburg University, Gothenburg, Sweden"},{"name":"Queen Silvia Children\u2019s Hospital, Sahlgrenska University Hospital, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aki","family":"Koivu","sequence":"additional","affiliation":[{"name":"Revvity, Diagnostics Research & Development, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikko","family":"Sairanen","sequence":"additional","affiliation":[{"name":"Revvity, Diagnostics Research & Development, Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mats","family":"Mellander","sequence":"additional","affiliation":[{"name":"Medical Faculty, Gothenburg University, Gothenburg, Sweden"},{"name":"Queen Silvia Children\u2019s Hospital, Sahlgrenska University Hospital, Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petru","family":"Liuba","sequence":"additional","affiliation":[{"name":"Medical Faculty, Lund University, Sweden"},{"name":"Children\u2019s Heart Centre, Skane\u2019s University Hospital, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"issue":"12","key":"zoi240593r1","doi-asserted-by":"publisher","first-page":"1890","DOI":"10.1016\/S0735-1097(02)01886-7","article-title":"The incidence of congenital heart disease.","volume":"39","author":"Hoffman","year":"2002","journal-title":"J Am Coll Cardiol"},{"issue":"2","key":"zoi240593r2","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1002\/bdr2.v114.2","article-title":"Prevalence of critical congenital heart defects and selected co-occurring congenital anomalies, 2014-2018: a U.S. population-based study.","volume":"114","author":"Stallings","year":"2022","journal-title":"Birth Defects Res"},{"issue":"21","key":"zoi240593r3","doi-asserted-by":"publisher","first-page":"2241","DOI":"10.1016\/j.jacc.2011.08.025","article-title":"Birth prevalence of congenital heart disease worldwide: a systematic review and meta-analysis.","volume":"58","author":"van der Linde","year":"2011","journal-title":"J Am Coll Cardiol"},{"issue":"3","key":"zoi240593r4","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S2352-4642(19)30402-X","article-title":"Global, regional, and national burden of congenital heart disease, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017.","volume":"4","author":"GBD 2017 Congenital Heart Disease Collaborators","year":"2020","journal-title":"Lancet Child Adolesc Health"},{"issue":"1","key":"zoi240593r5","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1186\/s12884-021-04028-5","article-title":"Prenatal detection of congenital heart disease\u2014results of a Swedish screening program 2013-2017.","volume":"21","author":"Waern","year":"2021","journal-title":"BMC Pregnancy Childbirth"},{"issue":"7","key":"zoi240593r6","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1007\/s00246-023-03197-8","article-title":"Impact of prenatal diagnosis of critical congenital heart disease on preoperative and postoperative outcomes.","volume":"44","author":"Dischinger","year":"2023","journal-title":"Pediatr Cardiol"},{"issue":"4","key":"zoi240593r7","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2023-061949","article-title":"Screening for critical congenital heart defects in Sweden.","volume":"152","author":"Lannering","year":"2023","journal-title":"Pediatrics"},{"issue":"4","key":"zoi240593r8","doi-asserted-by":"publisher","first-page":"252","DOI":"10.5830\/CVJA-2017-053","article-title":"The challenge in diagnosing coarctation of the aorta.","volume":"29","author":"Hoffman","year":"2018","journal-title":"Cardiovasc J Afr"},{"issue":"3","key":"zoi240593r9","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD011912.pub2","article-title":"Pulse oximetry screening for critical congenital heart defects.","volume":"3","author":"Plana","year":"2018","journal-title":"Cochrane Database Syst Rev"},{"issue":"11","key":"zoi240593r10","doi-asserted-by":"publisher","first-page":"2078","DOI":"10.1002\/ejhf.v22.11","article-title":"Circulating levels and prognostic value of soluble ST2 in heart failure are less influenced by age than N-terminal pro-B-type natriuretic peptide and high-sensitivity troponin T.","volume":"22","author":"Aimo","year":"2020","journal-title":"Eur J Heart Fail"},{"issue":"4","key":"zoi240593r11","doi-asserted-by":"publisher","first-page":"2084","DOI":"10.1002\/ehf2.v9.4","article-title":"Circulating levels and prognostic cut-offs of sST2, hs-cTnT, and NT-proBNP in women vs. men with chronic heart failure.","volume":"9","author":"Vergaro","year":"2022","journal-title":"ESC Heart Fail"},{"issue":"9","key":"zoi240593r12","doi-asserted-by":"publisher","first-page":"905","DOI":"10.17219\/acem\/131746","article-title":"Is N-terminal pro-brain type natriuretic peptide a useful marker in newborns with heart defects?","volume":"30","author":"Tarkowska","year":"2021","journal-title":"Adv Clin Exp Med"},{"issue":"4","key":"zoi240593r13","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1017\/S1047951123000331","article-title":"Paediatric heart failure\u2014understanding the pathophysiology and the current role of cardiac biomarkers in clinical practice.","volume":"33","author":"McGinn","year":"2023","journal-title":"Cardiol Young"},{"issue":"8","key":"zoi240593r14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0202406","article-title":"Predictive value of soluble ST2 in adolescent and adult patients with complex congenital heart disease.","volume":"13","author":"Laqqan","year":"2018","journal-title":"PLoS One"},{"issue":"10","key":"zoi240593r15","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1002\/ejhf.v24.10","article-title":"Congestion in heart failure: a circulating biomarker-based perspective. A review from the Biomarkers Working Group of the Heart Failure Association, European Society of Cardiology.","volume":"24","author":"N\u00fa\u00f1ez","year":"2022","journal-title":"Eur J Heart Fail"},{"issue":"2","key":"zoi240593r16","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/j.amjcard.2010.09.011","article-title":"Usefulness of soluble concentrations of interleukin family member ST2 as predictor of mortality in patients with acutely decompensated heart failure relative to left ventricular ejection fraction.","volume":"107","author":"Manzano-Fern\u00e1ndez","year":"2011","journal-title":"Am J Cardiol"},{"issue":"20","key":"zoi240593r17","doi-asserted-by":"publisher","first-page":"2191","DOI":"10.1001\/jama.2013.281053","article-title":"World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects.","volume":"310","author":"World Medical Association","year":"2013","journal-title":"JAMA"},{"issue":"6","key":"zoi240593r18","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1016\/S0197-2456(98)00037-3","article-title":"Power and sample size calculations for studies involving linear regression.","volume":"19","author":"Dupont","year":"1998","journal-title":"Control Clin Trials"},{"issue":"2","key":"zoi240593r19","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/0197-2456(90)90005-M","article-title":"Power and sample size calculations. A review and computer program.","volume":"11","author":"Dupont","year":"1990","journal-title":"Control Clin Trials"},{"issue":"12","key":"zoi240593r20","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2020.27561","article-title":"Evaluation of circulating cardiovascular biomarker levels for early detection of congenital heart disease in newborns in Sweden.","volume":"3","author":"Clausen","year":"2020","journal-title":"JAMA Netw Open"},{"issue":"2","key":"zoi240593r22","doi-asserted-by":"publisher","first-page":"e510","DOI":"10.1542\/peds.2014-1995","article-title":"Diagnosing significant PDA using natriuretic peptides in preterm neonates: a systematic review.","volume":"135","author":"Kulkarni","year":"2015","journal-title":"Pediatrics"},{"issue":"23","key":"zoi240593r23","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000020593","article-title":"Incidence and mortality trend of congenital heart disease at the global, regional, and national level, 1990-2017.","volume":"99","author":"Wu","year":"2020","journal-title":"Medicine (Baltimore)"},{"issue":"2","key":"zoi240593r24","doi-asserted-by":"publisher","first-page":"256","DOI":"10.4269\/ajtmh.17-0889","article-title":"Dried blood spots for global health diagnostics and surveillance: opportunities and challenges.","volume":"99","author":"Lim","year":"2018","journal-title":"Am J Trop Med Hyg"},{"issue":"8","key":"zoi240593r25","doi-asserted-by":"publisher","first-page":"2241","DOI":"10.21037\/tp","article-title":"Impacts of prenatal diagnosis of congenital heart diseases on outcomes.","volume":"10","author":"Bonnet","year":"2021","journal-title":"Transl Pediatr"},{"issue":"1","key":"zoi240593r27","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/s12911-021-01738-w","article-title":"Bayesian updating and sequential testing: overcoming inferential limitations of screening tests.","volume":"22","author":"Balayla","year":"2022","journal-title":"BMC Med Inform Decis Mak"},{"issue":"7","key":"zoi240593r28","doi-asserted-by":"publisher","first-page":"1286","DOI":"10.1074\/mcp.RA117.000015","article-title":"Stability of proteins in dried blood spot biobanks.","volume":"16","author":"Bj\u00f6rkesten","year":"2017","journal-title":"Mol Cell Proteomics"},{"issue":"2","key":"zoi240593r29","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1007\/s10741-021-10105-w","article-title":"Biomarkers for the diagnosis and management of heart failure.","volume":"27","author":"Castiglione","year":"2022","journal-title":"Heart Fail Rev"},{"issue":"25","key":"zoi240593r30","doi-asserted-by":"publisher","first-page":"2357","DOI":"10.1016\/j.jacc.2007.09.021","article-title":"Natriuretic peptides.","volume":"50","author":"Daniels","year":"2007","journal-title":"J Am Coll Cardiol"},{"issue":"6","key":"zoi240593r31","doi-asserted-by":"publisher","first-page":"e137","DOI":"10.1161\/CIR.0000000000000509","article-title":"2017 ACC\/AHA\/HFSA focused update of the 2013 ACCF\/AHA Guideline for the Management of Heart Failure: a report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines and the Heart Failure Society of America.","volume":"136","author":"Yancy","year":"2017","journal-title":"Circulation"},{"issue":"1","key":"zoi240593r32","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1002\/ejhf.v24.1","article-title":"2021 ESC Guidelines for the diagnosis and treatment of acute and chronic heart failure: developed by the Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC). With the special contribution of the Heart Failure Association (HFA) of the ESC.","volume":"24","author":"McDonagh","year":"2022","journal-title":"Eur J Heart Fail"},{"key":"zoi240593r33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcha.2020.100553","article-title":"NT-proBNP for risk prediction of cardiovascular events and all-cause mortality: the getABI-study.","volume":"29","author":"Rudolf","year":"2020","journal-title":"Int J Cardiol Heart Vasc"},{"issue":"11","key":"zoi240593r34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207118","article-title":"In-hospital mortality is associated with high NT-proBNP level.","volume":"13","author":"Benmachiche","year":"2018","journal-title":"PLoS One"},{"issue":"5","key":"zoi240593r35","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1253\/circj.CJ-09-0535","article-title":"The role of N-terminal pro-B-type natriuretic peptide in the diagnosis of congestive heart failure in children\u2014correlation with the heart failure score and comparison with B-type natriuretic peptide.","volume":"74","author":"Sugimoto","year":"2010","journal-title":"Circ J"},{"issue":"11","key":"zoi240593r36","doi-asserted-by":"publisher","first-page":"1723","DOI":"10.1016\/j.amjcard.2016.08.053","article-title":"Usefulness of serial N-terminal Pro-B-type natriuretic peptide measurements to predict cardiac death in acute and chronic dilated cardiomyopathy in children.","volume":"118","author":"den Boer","year":"2016","journal-title":"Am J Cardiol"},{"issue":"4","key":"zoi240593r37","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1177\/2150135119842864","article-title":"The association between cardiac biomarker NT-proBNP and 30-day readmission or mortality after pediatric congenital heart surgery.","volume":"10","author":"Parker","year":"2019","journal-title":"World J Pediatr Congenit Heart Surg"},{"issue":"7","key":"zoi240593r38","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.008349","article-title":"Prognostic value of serial N-terminal pro-B-type natriuretic peptide measurements in adults with congenital heart disease.","volume":"7","author":"Baggen","year":"2018","journal-title":"J Am Heart Assoc"},{"issue":"5","key":"zoi240593r39","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.119.015022","article-title":"Associations between blood biomarkers, cardiac function, and adverse outcome in a young fontan cohort.","volume":"10","author":"van den Bosch","year":"2021","journal-title":"J Am Heart Assoc"},{"issue":"1","key":"zoi240593r40","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s00246-008-9258-4","article-title":"NT-pro-B-type natriuretic peptide in infants and children: reference values based on combined data from four studies.","volume":"30","author":"Nir","year":"2009","journal-title":"Pediatr Cardiol"},{"issue":"6","key":"zoi240593r41","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1007\/s00392-005-0246-x","article-title":"Reference values of NT-proBNP serum concentrations in the umbilical cord blood and in healthy neonates and children.","volume":"94","author":"Schwachtgen","year":"2005","journal-title":"Z Kardiol"},{"issue":"2","key":"zoi240593r42","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.immuni.2018.12.012","article-title":"Cell-type-specific interleukin 1 receptor 1 signaling in the brain regulates distinct neuroimmune activities.","volume":"50","author":"Liu","year":"2019","journal-title":"Immunity"},{"key":"zoi240593r43","doi-asserted-by":"publisher","first-page":"475","DOI":"10.3389\/fimmu.2017.00475","article-title":"The ST2\/IL-33 axis in immune cells during inflammatory diseases.","volume":"8","author":"Griesenauer","year":"2017","journal-title":"Front Immunol"},{"key":"zoi240593r44","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2022.155891","article-title":"Interleukin-33 (IL-33): a critical review of its biology and the mechanisms involved in its release as a potent extracellular cytokine.","volume":"156","author":"Cayrol","year":"2022","journal-title":"Cytokine"},{"issue":"7","key":"zoi240593r45","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1358\/dot.2012.48.7.1811719","article-title":"Heart failure biomarkers: focus on interleukin-1 receptor-like 1-based blood tests.","volume":"48","author":"Broch","year":"2012","journal-title":"Drugs Today (Barc)"},{"issue":"11","key":"zoi240593r46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0259026","article-title":"Role of the IL-33\/ST2 axis in cardiovascular disease: a systematic review and meta-analysis.","volume":"16","author":"Sun","year":"2021","journal-title":"PLoS One"},{"key":"zoi240593r47","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/j.cca.2020.04.011","article-title":"Soluble ST2: a complex and diverse role in several diseases.","volume":"507","author":"Homsak","year":"2020","journal-title":"Clin Chim Acta"},{"key":"zoi240593r48","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2019.105619","article-title":"ST2\/IL-33 signaling in cardiac fibrosis.","volume":"116","author":"Vianello","year":"2019","journal-title":"Int J Biochem Cell Biol"},{"issue":"1","key":"zoi240593r49","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.hfc.2017.08.005","article-title":"Soluble ST2 in heart failure.","volume":"14","author":"McCarthy","year":"2018","journal-title":"Heart Fail Clin"},{"key":"zoi240593r50","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.cca.2014.09.021","article-title":"Soluble ST2 in heart failure.","volume":"443","author":"Dieplinger","year":"2015","journal-title":"Clin Chim Acta"},{"issue":"16","key":"zoi240593r51","doi-asserted-by":"publisher","first-page":"8900","DOI":"10.3390\/ijms23168900","article-title":"IL11 stimulates IL33 expression and proinflammatory fibroblast activation across tissues.","volume":"23","author":"Widjaja","year":"2022","journal-title":"Int J Mol Sci"},{"issue":"16","key":"zoi240593r52","doi-asserted-by":"publisher","first-page":"4693","DOI":"10.3390\/jcm11164693","article-title":"Soluble isoform of suppression of tumorigenicity 2 (ST2) biomarker in a large cohort of healthy pediatric population: determination of reference intervals.","volume":"11","author":"Perrone","year":"2022","journal-title":"J Clin Med"},{"issue":"18","key":"zoi240593r53","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1016\/j.clinbiochem.2015.08.007","article-title":"Soluble ST2 and galectin-3 in pediatric patients without heart failure.","volume":"48","author":"Meeusen","year":"2015","journal-title":"Clin Biochem"},{"issue":"6","key":"zoi240593r54","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.3390\/biomedicines11061676","article-title":"The ST2\/IL-33 pathway in adult and paediatric heart disease and transplantation.","volume":"11","author":"Brunetti","year":"2023","journal-title":"Biomedicines"},{"issue":"6","key":"zoi240593r55","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1016\/j.cjca.2019.02.016","article-title":"Association of soluble ST2 serum levels with outcomes in pediatric dilated cardiomyopathy.","volume":"35","author":"You","year":"2019","journal-title":"Can J Cardiol"},{"issue":"2","key":"zoi240593r56","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1093\/eurjpc\/zwac227","article-title":"Congenital heart disease: changes in recorded birth prevalence and cardiac interventions over the past half-century in Sweden.","volume":"30","author":"Giang","year":"2023","journal-title":"Eur J Prev Cardiol"},{"issue":"17","key":"zoi240593r57","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.4155\/bio.13.170","article-title":"The effect of hematocrit on bioanalysis of DBS: results from the EBF DBS-microsampling consortium.","volume":"5","author":"de Vries","year":"2013","journal-title":"Bioanalysis"},{"issue":"3","key":"zoi240593r58","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1177\/00045632231156035","article-title":"Cross-sectional audit assessing the quality of dried bloodspot specimens received by UK metabolic biochemistry laboratories for the biochemical monitoring of individuals with Phenylketonuria.","volume":"60","author":"Hogg","year":"2023","journal-title":"Ann Clin Biochem"},{"key":"zoi240593r21","doi-asserted-by":"crossref","DOI":"10.1002\/9781118548387","volume-title":"Applied Logistic Regression, Vol 398","author":"Hosmer","year":"2013"},{"key":"zoi240593r26","volume-title":"Principles and Practice of Screening for Disease","author":"Wilson","year":"1968"}],"container-title":["JAMA Network Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jamanetwork.com\/journals\/jamanetworkopen\/articlepdf\/2820206\/clausen_2024_oi_240593_1718293073.96929.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T15:03:28Z","timestamp":1719241408000},"score":1,"resource":{"primary":{"URL":"https:\/\/jamanetwork.com\/journals\/jamanetworkopen\/fullarticle\/2820206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,24]]},"references-count":58,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6,3]]}},"URL":"https:\/\/doi.org\/10.1001\/jamanetworkopen.2024.18097","relation":{},"ISSN":["2574-3805"],"issn-type":[{"value":"2574-3805","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,24]]}}}