{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T13:19:35Z","timestamp":1780406375966,"version":"3.54.1"},"reference-count":34,"publisher":"American Medical Association (AMA)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAMA Otolaryngol Head Neck Surg"],"published-print":{"date-parts":[[2018,11,1]]},"DOI":"10.1001\/jamaoto.2018.0602","type":"journal-article","created":{"date-parts":[[2018,4,18]],"date-time":"2018-04-18T19:45:25Z","timestamp":1524080725000},"page":"967","source":"Crossref","is-referenced-by-count":20,"title":["RNA Oncoimmune Phenotyping of HPV-Positive p16-Positive Oropharyngeal Squamous Cell Carcinomas by Nodal Status"],"prefix":"10.1001","volume":"144","author":[{"given":"Wesley H.","family":"Stepp","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, University of North Carolina, School of Medicine, Chapel Hill"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Farquhar","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of North Carolina, School of Medicine, Chapel Hill"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siddharth","family":"Sheth","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology, Department of Medicine, University of North Carolina, School of Medicine, Chapel Hill"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Mazul","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, University of North Carolina, Gillings School of Public Health, Chapel Hill"},{"name":"now at Division of Hematology-Oncology, Department of Medicine, University of Tennessee Health Science Center, Memphis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Mamdani","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of North Carolina, School of Medicine, Chapel Hill"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor G.","family":"Hackman","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, University of North Carolina, School of Medicine, Chapel Hill"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Neil","family":"Hayes","sequence":"additional","affiliation":[{"name":"Division of Medical Oncology, Department of Medicine, University of North Carolina, School of Medicine, Chapel Hill"},{"name":"now at Division of Hematology-Oncology, Department of Medicine, University of Tennessee Health Science Center, Memphis"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose P.","family":"Zevallos","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, University of North Carolina, Gillings School of Public Health, Chapel Hill"},{"name":"now at Department of Otolaryngology, Washington University in St Louis, School of Medicine, St Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10","reference":[{"issue":"1","key":"ooi180025r1","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1056\/NEJMoa0912217","article-title":"Human papillomavirus and survival of patients with oropharyngeal cancer.","volume":"363","author":"Ang","year":"2010","journal-title":"N Engl J Med"},{"issue":"5","key":"ooi180025r2","doi-asserted-by":"publisher","first-page":"886","DOI":"10.1038\/sj.bjc.6606091","article-title":"Head and neck cancer in Australia between 1982 and 2005 show increasing incidence of potentially HPV-associated oropharyngeal cancers.","volume":"104","author":"Hocking","year":"2011","journal-title":"Br J Cancer"},{"issue":"12","key":"ooi180025r3","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1001\/jamaoncol.2016.1804","article-title":"Incidence of oropharyngeal cancer among elderly patients in the United States.","volume":"2","author":"Zumsteg","year":"2016","journal-title":"JAMA Oncol"},{"issue":"6","key":"ooi180025r4","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1053\/j.seminoncol.2004.09.011","article-title":"Human papillomavirus-associated head and neck cancer is a distinct epidemiologic, clinical, and molecular entity.","volume":"31","author":"Gillison","year":"2004","journal-title":"Semin Oncol"},{"issue":"9420","key":"ooi180025r5","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1016\/S0140-6736(04)16194-1","article-title":"A causal role for human papillomavirus in head and neck cancer.","volume":"363","author":"Gillison","year":"2004","journal-title":"Lancet"},{"issue":"8","key":"ooi180025r6","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1002\/ijc.v120:8","article-title":"Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer.","volume":"120","author":"Reimers","year":"2007","journal-title":"Int J Cancer"},{"issue":"11","key":"ooi180025r7","doi-asserted-by":"publisher","first-page":"2645","DOI":"10.1002\/cncr.v116:11","article-title":"Intensity-modulated radiotherapy outcomes for oropharyngeal squamous cell carcinoma patients stratified by p16 status.","volume":"116","author":"Shoushtari","year":"2010","journal-title":"Cancer"},{"issue":"5","key":"ooi180025r8","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1200\/JCO.2004.00.3335","article-title":"Molecular classification identifies a subset of human papillomavirus\u2014associated oropharyngeal cancers with favorable prognosis.","volume":"24","author":"Weinberger","year":"2006","journal-title":"J Clin Oncol"},{"issue":"11","key":"ooi180025r9","doi-asserted-by":"publisher","first-page":"2101","DOI":"10.1093\/annonc\/mdu265","article-title":"The current and future impact of human papillomavirus on treatment of squamous cell carcinoma of the head and neck.","volume":"25","author":"Psyrri","year":"2014","journal-title":"Ann Oncol"},{"issue":"2","key":"ooi180025r10","first-page":"132","article-title":"Prognostic significance of p16 cellular localization in oropharyngeal squamous cell carcinoma.","volume":"46","author":"Lai","year":"2016","journal-title":"Ann Clin Lab Sci"},{"issue":"3","key":"ooi180025r11","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/bjc.2012.264","article-title":"Different cellular p16(INK4a) localisation may signal different survival outcomes in head and neck cancer.","volume":"107","author":"Zhao","year":"2012","journal-title":"Br J Cancer"},{"issue":"5","key":"ooi180025r12","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/j.ijrobp.2015.08.033","article-title":"Phase 2 trial of de-intensified chemoradiation therapy for favorable-risk human papillomavirus-associated oropharyngeal squamous cell carcinoma.","volume":"93","author":"Chera","year":"2015","journal-title":"Int J Radiat Oncol Biol Phys"},{"issue":"5","key":"ooi180025r13","doi-asserted-by":"publisher","first-page":"2229","DOI":"10.1007\/s00405-016-4433-3","article-title":"Primary transoral robotic surgery with concurrent neck dissection for early stage oropharyngeal squamous cell carcinoma implemented at a Danish head and neck cancer center: a phase II trial on feasibility and tumour margin status.","volume":"274","author":"Rubek","year":"2017","journal-title":"Eur Arch Otorhinolaryngol"},{"issue":"5","key":"ooi180025r14","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1200\/JCO.2012.44.0164","article-title":"Deintensification candidate subgroups in human papillomavirus-related oropharyngeal cancer according to minimal risk of distant metastasis.","volume":"31","author":"O\u2019Sullivan","year":"2013","journal-title":"J Clin Oncol"},{"issue":"3","key":"ooi180025r15","first-page":"885","article-title":"Local recurrence in head and neck cancer: relationship to radiation resistance and signal transduction.","volume":"8","author":"Gupta","year":"2002","journal-title":"Clin Cancer Res"},{"issue":"17","key":"ooi180025r16","doi-asserted-by":"publisher","first-page":"24194","DOI":"10.18632\/oncotarget.v7i17","article-title":"Metastatic model of HPV+ oropharyngeal squamous cell carcinoma demonstrates heterogeneity in tumor metastasis.","volume":"7","author":"Vermeer","year":"2016","journal-title":"Oncotarget"},{"issue":"6","key":"ooi180025r17","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1001\/jamaoto.2015.0347","article-title":"Transoral robotic surgery alone for oropharyngeal cancer: quality-of-life outcomes.","volume":"141","author":"Choby","year":"2015","journal-title":"JAMA Otolaryngol Head Neck Surg"},{"issue":"7","key":"ooi180025r18","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1001\/archoto.2012.1166","article-title":"Transoral robotic surgery alone for oropharyngeal cancer: an analysis of local control.","volume":"138","author":"Weinstein","year":"2012","journal-title":"Arch Otolaryngol Head Neck Surg"},{"issue":"1","key":"ooi180025r19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.oraloncology.2013.10.008","article-title":"Human papilloma virus testing in oropharyngeal squamous cell carcinoma: what the clinician should know.","volume":"50","author":"Mirghani","year":"2014","journal-title":"Oral Oncol"},{"key":"ooi180025r20","article-title":"Human Papillomavirus testing in head and neck carcinomas: guideline from the College of American Pathologists.","author":"Lewis","year":"2017","journal-title":"Arch Pathol Lab Med"},{"issue":"4","key":"ooi180025r21","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1097\/01.mlg.0000161338.54515.b1","article-title":"The influence of lymph node metastasis in the treatment of squamous cell carcinoma of the oral cavity, oropharynx, larynx, and hypopharynx: N0 versus N+.","volume":"115","author":"Layland","year":"2005","journal-title":"Laryngoscope"},{"issue":"2","key":"ooi180025r22","doi-asserted-by":"publisher","first-page":"122","DOI":"10.3322\/caac.21389","article-title":"Head and Neck cancers-major changes in the American Joint Committee on cancer eighth edition cancer staging manual.","volume":"67","author":"Lydiatt","year":"2017","journal-title":"CA Cancer J Clin"},{"issue":"3","key":"ooi180025r23","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1038\/nbt1385","article-title":"Direct multiplexed measurement of gene expression with color-coded probe pairs.","volume":"26","author":"Geiss","year":"2008","journal-title":"Nat Biotechnol"},{"issue":"8","key":"ooi180025r24","doi-asserted-by":"publisher","first-page":"2930","DOI":"10.1172\/JCI91190","article-title":"IFN-?-related mRNA profile predicts clinical response to PD-1 blockade.","volume":"127","author":"Ayers","year":"2017","journal-title":"J Clin Invest"},{"issue":"1","key":"ooi180025r25","doi-asserted-by":"publisher","first-page":"E14","DOI":"10.3390\/biomedicines6010014","article-title":"Bringing the next generation of immuno-oncology biomarkers to the clinic.","volume":"6","author":"Cesano","year":"2018","journal-title":"Biomedicines"},{"key":"ooi180025r26","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/s40425-017-0215-8","article-title":"Gene expression markers of tumor infiltrating leukocytes.","volume":"5","author":"Danaher","year":"2017","journal-title":"J Immunother Cancer"},{"key":"ooi180025r27","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.oraloncology.2017.02.004","article-title":"Human papillomavirus (HPV) 16 antibodies at diagnosis of HPV-related oropharyngeal cancer and antibody trajectories after treatment.","volume":"67","author":"Zhang","year":"2017","journal-title":"Oral Oncol"},{"key":"ooi180025r28","article-title":"A correlative analysis of PD-L1, PD-1, PD-L2, EGFR, HER2, and HER3 expression in oropharyngeal squamous cell carcinoma","author":"Steuer","journal-title":"Mol Cancer Ther"},{"key":"ooi180025r29","article-title":"Development and validation of a radiomic signature to predict HPV (p16) status from standard CT imaging: a multicenter study","author":"Leijenaar","journal-title":"Br J Radiol"},{"issue":"4","key":"ooi180025r30","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1158\/1078-0432.CCR-14-2481","article-title":"Integrative analysis of head and neck cancer identifies two biologically distinct HPV and three non-HPV subtypes.","volume":"21","author":"Keck","year":"2015","journal-title":"Clin Cancer Res"},{"issue":"7536","key":"ooi180025r31","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1038\/nature14129","article-title":"Comprehensive genomic characterization of head and neck squamous cell carcinomas.","volume":"517","author":"Cancer Genome Atlas","year":"2015","journal-title":"Nature"},{"issue":"5","key":"ooi180025r32","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1200\/JCO.2016.68.3300","article-title":"E1308: phase II trial of induction chemotherapy followed by reduced-dose radiation and weekly cetuximab in patients with HPV-associated resectable squamous cell carcinoma of the oropharynx: ECOG-ACRIN Cancer Research Group.","volume":"35","author":"Marur","year":"2017","journal-title":"J Clin Oncol"},{"issue":"8","key":"ooi180025r33","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.1200\/JCO.2008.18.1370","article-title":"Supervised risk predictor of breast cancer based on intrinsic subtypes.","volume":"27","author":"Parker","year":"2009","journal-title":"J Clin Oncol"},{"key":"ooi180025r34","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1186\/s12920-015-0129-6","article-title":"Development and verification of the PAM50-based Prosigna breast cancer gene signature assay.","volume":"8","author":"Wallden","year":"2015","journal-title":"BMC Med Genomics"}],"container-title":["JAMA Otolaryngology\u2013Head & Neck Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/jamanetwork.com\/journals\/jamaotolaryngology\/fullarticle\/2678979","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T00:00:35Z","timestamp":1542412835000},"score":1,"resource":{"primary":{"URL":"http:\/\/archotol.jamanetwork.com\/article.aspx?doi=10.1001\/jamaoto.2018.0602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,1]]},"references-count":34,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11,1]]}},"URL":"https:\/\/doi.org\/10.1001\/jamaoto.2018.0602","relation":{},"ISSN":["2168-6181"],"issn-type":[{"value":"2168-6181","type":"print"}],"subject":[],"published":{"date-parts":[[2018,11,1]]}}}