{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T03:37:39Z","timestamp":1793763459566,"version":"4.6.1"},"reference-count":15,"publisher":"American Medical Association (AMA)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAMA Otolaryngol Head Neck Surg"],"published-print":{"date-parts":[[2026,7,1]]},"abstract":"<jats:sec id=\"ab-ooi260019-4\">\n                    <jats:title>Importance<\/jats:title>\n                    <jats:p>\n                      Tests for total antibodies specific to the Epstein-Barr virus (EBV)\n                      <jats:italic toggle=\"yes\">BNLF2b<\/jats:italic>\n                      gene\u2013encoded putative protein (P85-Ab) have shown high sensitivity and specificity for detecting newly diagnosed nasopharyngeal carcinoma (NPC). However, independent validation of these findings is important before considering clinical application.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"ab-ooi260019-5\">\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To compare the performance of P85-Ab testing for NPC detection with a combined Epstein-Barr virus\u2013specific IgA antibody score and a circulating EBV DNA algorithm.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"ab-ooi260019-6\">\n                    <jats:title>Design, Setting, and Participants<\/jats:title>\n                    <jats:p>This was a multicenter case-control study conducted from July 2010 to December 2014 at 6 medical centers in northern and central Taiwan. Participants were patients with NPC and controls who all provided blood samples for EBV biomarker testing. Data were analyzed from January 2025 to January 2026.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"ab-ooi260019-7\">\n                    <jats:title>Exposures<\/jats:title>\n                    <jats:p>Total antibodies specific to P85-Ab. For comparison, EBV viral capsid antigen\/nuclear antigen 1 (VCA-IgA\/EBNA1-IgA) antibody score and the circulating EBV DNA algorithm results were evaluated from the same archived blood specimens collected at enrollment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"ab-ooi260019-8\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The analysis included 892 patients with NPC (mean [SD] age, 48.6 [11.1] years; 191 females [21.4%] and 701 males [78.6%]) and 1804 individuals in the control group (mean [SD] age, 48.1 [12.2] years; 542 females [30.0%] and 1262 males [70.0%]). P85-Ab demonstrated a sensitivity of 92.5% (95% CI, 90.7%-94.3%) and specificity of 98.7% (95% CI, 98.2%-99.2%), higher than the EBV VCA-IgA\/EBNA1-IgA score (sensitivity, 88.4%; specificity, 94.9%) and comparable to the circulating EBV DNA algorithm (sensitivity, 93.2%; specificity, 98.1%). Sensitivity for early-stage NPC was higher for P85-Ab (93%) than for the other 2 methods (87%). P85-Ab performance remained high across age, sex, region, ethnicity, family history, and smoking subgroups. At NPC incidence rates of 20 to 100 per 100\u202f000 person-years, the numbers needed to screen were similar across the 3 approaches: 5656 and 1131 for EBV VCA-IgA\/EBNA1-IgA; 5365 and 1073 for EBV DNA; and 5405 and 1081 for P85-Ab. Positive predictive value (PPV) was 0.4% for the EBV antibody score, 1.0% for the EBV DNA algorithm, and 1.4% for the P85-Ab test; at an incidence of 100 per 100 000, the PPVs increased to 1.7%, 4.7%, and 6.6%, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"ab-ooi260019-9\">\n                    <jats:title>Conclusions and Relevance<\/jats:title>\n                    <jats:p>This multicenter case-control study found that P85-Ab testing had high sensitivity and specificity for early detection of NPC, with performance better than or comparable to existing EBV-based screening methods. These findings support the potential use of P85-Ab testing as a practical biomarker for population-based NPC screening.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1001\/jamaoto.2026.0838","type":"journal-article","created":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:00:35Z","timestamp":1779375635000},"page":"669","source":"Crossref","is-referenced-by-count":1,"title":["Novel Anti-\n                    <i>BNLF2b<\/i>\n                    Antibody Screening and Early Detection of Nasopharyngeal Carcinoma"],"prefix":"10.1001","volume":"152","author":[{"given":"Wan-Lun","family":"Hsu","sequence":"first","affiliation":[{"name":"Data Science Center, College of Medicine, Fu-Jen Catholic University, New Taipei City, Taiwan"},{"name":"Master Program of Big Data in Medical Healthcare Industry, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabao","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Emerging Infectious Diseases and Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China"},{"name":"Centre for Virology, Vaccinology, and Therapeutics Limited, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoi Yan","family":"Lam","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Cancer Epidemiology and Genetics, US National Cancer Institute, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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