{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:26:29Z","timestamp":1792070789374,"version":"4.4.0"},"reference-count":25,"publisher":"American Medical Association (AMA)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAMA Pediatr"],"published-print":{"date-parts":[[2025,6,1]]},"abstract":"<jats:sec id=\"ab-poi250004-4\"><jats:title>Importance<\/jats:title><jats:p>An emerging paradigm attributes third-trimester fetal growth deceleration in uncomplicated twin pregnancies to an evolutionary adaptive process. Evaluating longitudinal fetal soft-tissue development may provide important insights into differential fetal growth trajectories between twins and singletons.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-5\"><jats:title>Objective<\/jats:title><jats:p>To compare twin vs singleton lean and fat tissue growth across pregnancy.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-6\"><jats:title>Design, Setting, and Participants<\/jats:title><jats:p>Prospective cohort study of dichorionic twins and singletons with serial ultrasound scans to chart fetal growth (2009-2013) and collect fetal volume data to measure fat and lean tissue (2015-2019) in 12 US clinical centers. Participants were individuals with singleton (n\u2009=\u20092802 enrolled) and twin (n\u2009=\u2009171 pairs enrolled) pregnancies who generally had no chronic disease. Data analysis was performed from September 2023 to June 2024.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-7\"><jats:title>Exposure<\/jats:title><jats:p>Twin status.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-8\"><jats:title>Main Outcomes and Measures<\/jats:title><jats:p>Abdominal area, maximum abdominal subcutaneous tissue thickness, fractional thigh volume, fractional lean thigh volume, fractional fat thigh volume, midthigh area (including lean and fat components), ratio of fractional fat thigh volume to fractional thigh volume, and ratio of midthigh fat area to midthigh area, measured up to 6 times between 15 and 37 weeks\u2019 gestation.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-9\"><jats:title>Results<\/jats:title><jats:p>Analyses included 315 twin and 2604 singleton fetuses. The mean (SD) maternal age at delivery was 31.3 (6.1) years for twins and 28.2 (5.5) years for singletons, with a mean (SD) gestational age at delivery of 35.2 (4.2) weeks for twins and 39.2 (1.7) weeks for singletons. Mean twin abdominal measurements were significantly smaller than those of singletons between 25 and 37 weeks\u2019 gestation for area (difference at 25 weeks, \u221248.6 [95% CI, \u2212102.2 to \u22125.1] mm<jats:sup>2<\/jats:sup>; difference at 37 weeks, \u2212480.5 [95% CI, \u2212677.2 to \u2212283.5] mm<jats:sup>2<\/jats:sup>) and between 27 and 37 weeks for maximum abdominal subcutaneous tissue thickness (difference at 27 weeks, \u22120.13 [95% CI, \u22120.24 to \u22120.02] mm; difference at 37 weeks, \u22120.40 [95% CI, \u22120.68 to \u22120.13] mm). Beginning at 15 weeks, fractional thigh volumes were significantly smaller for twins (mean fractional thigh volume difference, \u22120.11 [95% CI, \u22120.16 to \u22120.07] cm<jats:sup>3<\/jats:sup>; mean fractional fat thigh volume difference, \u22120.08 [95% CI,\u22120.12 to \u22120.05] cm<jats:sup>3<\/jats:sup>) relative to singletons, persisting through 37 weeks (mean fractional thigh volume difference, \u22127.55 [95% CI, \u221211.76 to \u22123.34] cm<jats:sup>3<\/jats:sup>; mean fractional fat thigh volume difference, \u22125.60 [95% CI, \u22128.37 to \u22122.82] cm<jats:sup>3<\/jats:sup>). Mean fractional lean thigh volume was significantly smaller for twins at 15 to 16 and 23 to 36 weeks. For the ratio of fractional fat thigh volume to fractional thigh volume, twins had a 2.7% to 4.2% smaller fat percentage between 15 and 37 weeks compared with singletons.<\/jats:p><\/jats:sec><jats:sec id=\"ab-poi250004-10\"><jats:title>Conclusions and Relevance<\/jats:title><jats:p>Twins had proportionally less fat tissue accumulation in utero compared with singletons as early as 15 weeks\u2019 gestation, when competition for nutritional resources was low. Persistent findings of smaller twin sizes and less fat accumulation across pregnancy support the concept of an early evolutionary adaptive process in otherwise uncomplicated dichorionic twin growth.<\/jats:p><\/jats:sec>","DOI":"10.1001\/jamapediatrics.2025.0116","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T15:03:34Z","timestamp":1744038214000},"page":"630","source":"Crossref","is-referenced-by-count":8,"title":["Fetal Body Composition in Twins and Singletons"],"prefix":"10.1001","volume":"179","author":[{"given":"Jessica L.","family":"Gleason","sequence":"first","affiliation":[{"name":"Epidemiology Branch, Division of Population Health Research, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and 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