{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T08:49:01Z","timestamp":1779353341233,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"10B","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":5891,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"DOI":"10.1002\/(sici)1097-0061(199907)15:10b<963::aid-yea399>3.0.co;2-w","type":"journal-article","created":{"date-parts":[[2002,9,10]],"date-time":"2002-09-10T18:13:32Z","timestamp":1031681612000},"page":"963-972","source":"Crossref","is-referenced-by-count":809,"title":["Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines"],"prefix":"10.1002","volume":"15","author":[{"given":"Michael","family":"Knop","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katja","family":"Siegers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gislene","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Zachariae","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Winsor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Nasmyth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elmar","family":"Schiebel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,7,16]]},"reference":[{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB1|cit1","doi-asserted-by":"crossref","first-page":"3329","DOI":"10.1093\/nar\/21.14.3329","article-title":"A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae","volume":"21","author":"Baudin","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB2|cit2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1099\/00221287-143-2-303","article-title":"Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans","volume":"143","author":"Cormack","year":"1997","journal-title":"Microbiol."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB3|cit3","doi-asserted-by":"crossref","first-page":"887","DOI":"10.1083\/jcb.132.5.887","article-title":"Spc42p-a phosphorylated component of the Saccharomyces cerevisiae spindle pole body (SPB) with an essential function during SPB duplication","volume":"132","author":"Donaldson","year":"1996","journal-title":"J. Cell Biol."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB4|cit4","doi-asserted-by":"crossref","first-page":"546, 563","DOI":"10.1126\/science.274.5287.546","article-title":"Life with 6000 genes","volume":"274","author":"Goffeau","year":"1996","journal-title":"Science"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB5|cit5","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1002\/yea.320101007","article-title":"Rapid protein extraction from Saccharomyces cerevisiae","volume":"10","author":"Horvath","year":"1994","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB6|cit6","doi-asserted-by":"crossref","first-page":"4323","DOI":"10.1002\/j.1460-2075.1988.tb03331.x","article-title":"A novel nucleoskeletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae","volume":"7","author":"Hurt","year":"1988","journal-title":"EMBO J."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB7|cit7","doi-asserted-by":"crossref","first-page":"6985","DOI":"10.1093\/emboj\/16.23.6985","article-title":"Spc98p and Spc97p of the yeast \u03b3-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p","volume":"16","author":"Knop","year":"1997","journal-title":"EMBO J."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB8|cit8","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB9|cit9","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1089\/dna.1984.3.173","article-title":"Linker tailing: unphosphorylated linker oligonucleotides for joining DNA termini","volume":"3","author":"Lathe","year":"1984","journal-title":"DNA"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB10|cit10","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1002\/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U","article-title":"Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae","volume":"14","author":"Longtine","year":"1998","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB11|cit11","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1006\/abio.1994.1060","article-title":"Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase","volume":"216","author":"Parks","year":"1994","journal-title":"Anal. Biochem."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB12|cit12","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1002\/(SICI)1097-0061(19980915)14:12<1139::AID-YEA306>3.0.CO;2-B","article-title":"New constructs and strategies for efficient PCR-based gene manipulations in yeast","volume":"14","author":"Puig","year":"1998","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB13|cit13","doi-asserted-by":"crossref","first-page":"2161","DOI":"10.1002\/j.1460-2075.1983.tb01717.x","article-title":"Import of proteins into mitochondria: a 70 kilodalton outer membrane protein with a large carboxy-terminal deletion is still transported to the outer membrane","volume":"2","author":"Riezman","year":"1983","journal-title":"EMBO J."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB14|cit14","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.1083\/jcb.111.5.1913","article-title":"Components of the yeast spindle and spindle pole body","volume":"111","author":"Rout","year":"1990","journal-title":"J. Cell Biol."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB15|cit15","volume-title":"Molecular Cloning: A Laboratry Manual","author":"Sambrook","year":"1989"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB16|cit16","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/BF00340712","article-title":"High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier","volume":"16","author":"Schiestl","year":"1989","journal-title":"Curr. Genet."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB17|cit17","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1002\/yea.320111306","article-title":"Use of polymerase chain-reaction epitope tagging for protein tagging in Saccharomyces cerevisiae","volume":"11","author":"Schneider","year":"1995","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB18|cit18","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0076-6879(91)94004-V","article-title":"Getting started with yeast","volume":"194","author":"Sherman","year":"1991","journal-title":"Methods Enzymol."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB19|cit19","unstructured":"Siegers K. Waldmann T. Leroux M. R. Grein K. Shevchenko A. Schiebel E. Hartl F. U. 1998 Manuscript submitted"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB20|cit20","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1093\/genetics\/122.1.19","article-title":"A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae","volume":"122","author":"Sikorski","year":"1989","journal-title":"Genetics"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB21|cit21","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0378-1119(88)90005-4","article-title":"Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase","volume":"67","author":"Smith","year":"1988","journal-title":"Gene"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB22|cit22","doi-asserted-by":"crossref","DOI":"10.1002\/j.1460-2075.1993.tb05845.x","article-title":"Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins","author":"Tyers","year":"1993","journal-title":"EMBO J."},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB23|cit23","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/(SICI)1097-0061(19970915)13:11<1065::AID-YEA159>3.0.CO;2-K","article-title":"Heterologous His3 marker and GFP reporter modules for PCRtargeting in Saccharomyces cerevisiae","volume":"13","author":"Wach","year":"1997","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB24|cit24","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1002\/yea.320101310","article-title":"New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae","volume":"10","author":"Wach","year":"1994","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB25|cit25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1002\/yea.320050107","article-title":"Extraction and rapid inactivation of proteins from Saccharomyces cerevisiae by trichloroacetic acid precipitation","volume":"5","author":"Wright","year":"1989","journal-title":"Yeast"},{"key":"10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W-BIB26|cit26","doi-asserted-by":"crossref","first-page":"4819","DOI":"10.1073\/pnas.81.15.4819","article-title":"Two nuclear mutations that block mitochondrial protein import in yeast","volume":"81","author":"Yaffe","year":"1984","journal-title":"Proc. Natl Acad. Sci. USA"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F(SICI)1097-0061(199907)15:10B%3C963::AID-YEA399%3E3.0.CO;2-W","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1002\/(SICI)1097-0061(199907)15:10B%3C963::AID-YEA399%3E3.0.CO;2-W","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T02:55:18Z","timestamp":1680490518000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/(SICI)1097-0061(199907)15:10B<963::AID-YEA399>3.0.CO;2-W"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7,16]]},"references-count":26,"journal-issue":{"issue":"10B"},"URL":"https:\/\/doi.org\/10.1002\/(sici)1097-0061(199907)15:10b<963::aid-yea399>3.0.co;2-w","relation":{},"ISSN":["0749-503X"],"issn-type":[{"value":"0749-503X","type":"print"}],"subject":[],"published":{"date-parts":[[1999,7,16]]}}}