{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:36:00Z","timestamp":1767854160600,"version":"3.49.0"},"reference-count":4,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T00:00:00Z","timestamp":1133395200000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Toxicology"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protocol described in this unit is designed to evaluate the effects of androgenic and antiandrogenic endocrine\u2010disrupting compounds (EDCs) in the castrate\u2010immature male rat. Continuous 10\u2010day exposure of the prepubertal male to chemicals is used to identify androgenic or antiandrogenic activities based on the weights of several androgen\u2010dependent tissues on the day after treatment is ended. Androgen\u2010dependent organ weights and growth, along with kidney, liver, and adrenal weights are measured at necropsy. The androgen\u2010dependent tissues include the ventral prostate, seminal vesicles (with fluid plus coagulating glands), levator ani plus bulbocavernosus muscles, Cowper's glands, and glans penis. Optional measures, which facilitate interpretation of the data when positive effects are detected, include serum testosterone and luteinizing hormone levels. Androgenic chemicals cause increases in one or all of the androgen\u2010dependent tissue weights after being administered by oral gavage or subcutaneously (s.c.) to castrate\u2010immature male rats. Antiandrogenic chemicals cause reductions in androgen\u2010dependent tissue weights after being coadministered with testosterone propionate (s.c.).<\/jats:p>","DOI":"10.1002\/0471140856.tx1609s26","type":"journal-article","created":{"date-parts":[[2005,12,13]],"date-time":"2005-12-13T14:17:07Z","timestamp":1134483427000},"source":"Crossref","is-referenced-by-count":9,"title":["Hershberger Assay to Investigate the Effects of Endocrine\u2010Disrupting Compounds with Androgenic or Antiandrogenic Activity in Castrate\u2010Immature Male Rats"],"prefix":"10.1002","volume":"26","author":[{"suffix":"Jr.","given":"L. Earl","family":"Gray","sequence":"first","affiliation":[{"name":"United States Environmental Protection Agency, NHEERL  Research Triangle Park North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johnathan","family":"Furr","sequence":"additional","affiliation":[{"name":"United States Environmental Protection Agency, NHEERL  Research Triangle Park North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph S.","family":"Ostby","sequence":"additional","affiliation":[{"name":"United States Environmental Protection Agency, NHEERL  Research Triangle Park North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Standard Methods Adopted by Official Organization","author":"Dorfman R.I.","year":"1962"},{"key":"e_1_2_8_3_1","first-page":"131","article-title":"Evaluation of the rodent Hershberger bioassay using three reference (anti)androgens","volume":"56","author":"Freyberger A.","year":"2005","journal-title":"Arh. Hig. Rad. Toksikol."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-83-20301"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2003.10.012"}],"container-title":["Current Protocols in Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471140856.tx1609s26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471140856.tx1609s26","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471140856.tx1609s26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T11:40:02Z","timestamp":1693741202000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471140856.tx1609s26"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,11]]},"references-count":4,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["10.1002\/0471140856.tx1609s26"],"URL":"https:\/\/doi.org\/10.1002\/0471140856.tx1609s26","archive":["Portico"],"relation":{},"ISSN":["1934-9254","1934-9262"],"issn-type":[{"value":"1934-9254","type":"print"},{"value":"1934-9262","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,11]]}}}