{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:09:25Z","timestamp":1760216965450},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,6,1]],"date-time":"2005-06-01T00:00:00Z","timestamp":1117584000000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Toxicology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protein\u2010centered radicals are involved in biological oxidative damage induced by drugs, environmental hazards, and cellular reactive oxygen species. Presently, the technique most widely used to study protein\u2010centered radicals is electron spin resonance (ESR; also known as electron paramagnetic resonance, EPR); used either directly or in combination with the spin\u2010trapping technique. Protein\u2010centered radicals may be trapped with the nitrone spin trap 5,5\u2010dimethyl\u20101\u2010pyrroline <jats:italic>N<\/jats:italic>\u2010oxide (DMPO) forming DMPO\u2010radical adducts. However, after a few minutes these adducts decay, often by oxidation to DMPO\u2010protein radical\u2013derived nitrone adducts, which are ESR\u2010silent species. Because nitrone adducts are not free radicals and their formation involves the creation of a covalent linkage, they are stable long after the ESR signal decays. In the new alternative technique of immuno\u2010spin trapping, nitrone adducts are detected by using an antibody, i.e., anti\u2010DMPO, that recognizes their nitrone moiety. Immuno\u2010spin trapping is a simple, reliable, affordable, sensitive, and specific approach to detecting protein\u2010centered radicals, and its development brings the power of immunoassays to bear on the field of toxicology of free radical\u2013mediated biological damage.<\/jats:p>","DOI":"10.1002\/0471140856.tx1707s24","type":"journal-article","created":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T14:16:21Z","timestamp":1119968181000},"source":"Crossref","is-referenced-by-count":12,"title":["Immuno\u2010Spin Trapping: Detection of Protein\u2010Centered Radicals"],"prefix":"10.1002","volume":"24","author":[{"given":"Dario C.","family":"Ramirez","sequence":"first","affiliation":[{"name":"National Institute of Environmental Health Science, National Institutes of Health  Research Triangle Park North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald P.","family":"Mason","sequence":"additional","affiliation":[{"name":"National Institute of Environmental Health Science, National Institutes of Health  Research Triangle Park North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6]]},"reference":[{"key":"e_1_2_6_2_1_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1999.01009.x"},{"key":"e_1_2_6_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2004.07.013"},{"key":"e_1_2_6_2_3_1","doi-asserted-by":"publisher","DOI":"10.1039\/b103211a"},{"key":"e_1_2_6_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2003.12.013"},{"key":"e_1_2_6_2_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3240001"},{"key":"e_1_2_6_2_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310704200"},{"key":"e_1_2_6_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00895-X"},{"key":"e_1_2_6_2_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M310034200"},{"key":"e_1_2_6_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2728(00)00252-8"},{"key":"e_1_2_6_2_10_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2003)0770585UHRDBI2.0.CO2"},{"key":"e_1_2_6_2_11_1","first-page":"49","volume-title":"Toxicology of the Human Environment. 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