{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:59:36Z","timestamp":1792677576621,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T00:00:00Z","timestamp":1343779200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Protein Science"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nitrotyrosine is a post\u2010translationally modified amino acid with distinctly different properties than tyrosine or any other of the genetically encoded amino acids. Detecting proteins containing nitrotyrosine is the first step towards a better understanding of the role of nitrotyrosine in health and disease. Moreover, quantifying the extent of nitrotyrosine and determining its location in a protein forms the basis for a better understanding of the effect of tyrosine nitration on biological function. Described in this unit is a method to detect tyrosine\u2010nitrated proteins in tissue sections and on western blots after creating a fluorescent complex between aminotyrosine, salicylaldehyde, and Al<jats:sup>3+<\/jats:sup>. In addition, an approach is detailed for labeling aminotyrosine with biotin to enrich peptides from complex samples. Both methods require reduction of nitrotyrosine to aminotyrosine, which can be achieved with sodium dithionite or hemin plus dithiothreitol. <jats:italic>Curr. Protoc. Protein Sci<\/jats:italic>. 69:14.13.1\u201014.13.19. \u00a9 2012 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471140864.ps1413s69","type":"journal-article","created":{"date-parts":[[2012,8,1]],"date-time":"2012-08-01T12:05:57Z","timestamp":1343822757000},"source":"Crossref","is-referenced-by-count":12,"title":["Enrichment and Detection of Tyrosine\u2010Nitrated Proteins"],"prefix":"10.1002","volume":"69","author":[{"given":"Frank","family":"Dekker","sequence":"first","affiliation":[{"name":"Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy  University of Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Abello","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry, Groningen Research Institute of Pharmacy  University of Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalina","family":"Wisastra","sequence":"additional","affiliation":[{"name":"Pharmaceutical Gene Modulation, Groningen Research Institute of Pharmacy  University of Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer","family":"Bischoff","sequence":"additional","affiliation":[{"name":"Analytical Biochemistry, Groningen Research Institute of Pharmacy  University of Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070154e"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900039c"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2009.02.002"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00780-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/psc.1298"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199812)186:4<416::AID-PATH201>3.0.CO;2-U"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117607"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.4.9912064"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200107)44:7<1534::AID-ART276>3.0.CO;2-#"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(90)90173-K"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M110.002964"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100148"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-562-6_10"}],"container-title":["Current Protocols in Protein Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F0471140864.ps1413s69","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471140864.ps1413s69","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471140864.ps1413s69","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471140864.ps1413s69","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T19:13:47Z","timestamp":1692472427000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471140864.ps1413s69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1002\/0471140864.ps1413s69"],"URL":"https:\/\/doi.org\/10.1002\/0471140864.ps1413s69","archive":["Portico"],"relation":{},"ISSN":["1934-3655","1934-3663"],"issn-type":[{"value":"1934-3655","type":"print"},{"value":"1934-3663","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,8]]}}}