{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:12:59Z","timestamp":1762866779621},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,4]],"date-time":"2016-01-04T00:00:00Z","timestamp":1451865600000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Neuroscience"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use has been largely restricted to mammals. In order to explore the use of such viruses in an expanded host range, we tested the transsynaptic tracing ability of recombinant vesicular stomatitis virus (rVSV) vectors in a variety of organisms. Successful infection and gene expression were achieved in a wide range of organisms, including vertebrate and invertebrate model organisms. Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and\/or viral replication and transmission, was observed in Old and New World monkeys, seahorses, jellyfish, zebrafish, chickens, and mice. These vectors are widely applicable for gene delivery, afferent tract tracing, and\/or directional connectivity mapping. Here, we detail the use of these vectors and provide protocols for propagating virus, changing the surface glycoprotein, and infecting multiple organisms using several injection strategies. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142301.ns0126s74","type":"journal-article","created":{"date-parts":[[2016,1,4]],"date-time":"2016-01-04T19:08:43Z","timestamp":1451934523000},"source":"Crossref","is-referenced-by-count":15,"title":["Anterograde or Retrograde Transsynaptic Circuit Tracing in Vertebrates with Vesicular Stomatitis Virus Vectors"],"prefix":"10.1002","volume":"74","author":[{"given":"Kevin T.","family":"Beier","sequence":"first","affiliation":[{"name":"Department of Biology, Department of Psychiatry and Behavioral Sciences, Stanford University  Stanford California"},{"name":"These authors contributed equally to this unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan A.","family":"Mundell","sequence":"additional","affiliation":[{"name":"Department of Genetics, Department of Ophthalmology, Howard Hughes Medical Institute, Harvard Medical School  Boston Massachusetts"},{"name":"These authors contributed equally to this unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Albert","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Regenerative Medicine, Department of Neurology, James &amp; Jean Culver Vision Discovery Institute, Medical College of Georgia, Augusta University  Augusta Georgia"},{"name":"These authors contributed equally to this unit"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Constance L.","family":"Cepko","sequence":"additional","affiliation":[{"name":"Department of Genetics, Department of Ophthalmology, Howard Hughes Medical Institute, Harvard Medical School  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,4]]},"reference":[{"key":"e_1_2_10_1_2_1","volume-title":"Current Protocols in Molecular Biology","author":"Ausubel F.M.","year":"2015"},{"key":"e_1_2_10_1_3_1","first-page":"1","article-title":"Viral concentration determination through plaque assays: Using traditional and novel overlay systems","volume":"93","author":"Baer A.","year":"2014","journal-title":"J. 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