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LRPs can themselves undergo limited self\u2010renewal and ultimately differentiate into highly specialized cells that make up the nervous system. However, this physiologically delimited definition of NSCs and LRPs has become increasingly blurred due to lack of protocols for effectively separating these types of cells from primary tissues. This unit discusses recent attempts using fluorescence\u2010activated cell sorting (FACS) strategies to prospectively isolate NSCs from different types of LRPs as they appear in vivo, and details a protocol that optimally attains this goal. Thus, the strategy presented here provides a framework for more precise studies of NSC and LRP cell biology in the future, which can be applied to all vertebrates, including humans.<\/jats:p>","DOI":"10.1002\/0471142301.ns0318s33","type":"journal-article","created":{"date-parts":[[2005,12,1]],"date-time":"2005-12-01T18:04:16Z","timestamp":1133460256000},"source":"Crossref","is-referenced-by-count":7,"title":["Fluorescence\u2010Based Sorting of Neural Stem Cells and Progenitors"],"prefix":"10.1002","volume":"33","author":[{"given":"Dragan","family":"Maric","sequence":"first","affiliation":[{"name":"National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH)  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffery L.","family":"Barker","sequence":"additional","affiliation":[{"name":"National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH)  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90207-5"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35004599"},{"key":"e_1_2_6_4_1","volume-title":"Atlas of Prenatal Rat Brain Development","author":"Altman J.","year":"1995"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490210209"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8716"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0828"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(02)00835-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19990801)57:3<371::AID-JNR9>3.0.CO;2-O"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01872268"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.4.1797"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2003.12.012"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82109-3"},{"key":"e_1_2_6_14_1","volume-title":"Anatomy and Embryology of the Laboratory Rat","author":"Hebel R.","year":"1986"},{"key":"e_1_2_6_15_1","first-page":"301","article-title":"Phylogenetic recapitulation of brain ganglioside composition during ontogenetic development","volume":"68","author":"Hilbig R.","year":"1981","journal-title":"Comp. 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