{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T07:40:12Z","timestamp":1692517212418},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":455,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Nucleic Acid Chemistry"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protocols for postsynthetic modification of 2\u2010amino\u2010containing oligoribonucleotides with either an alkyl\u2010phenyl disulfide or an alkyl thiol group are described. These groups react under mild conditions to form disulfide cross\u2010links by thiol\u2010disulfide interchange. These reactants do not form a disulfide bond when incorporated on opposite faces of a short continuous RNA helix, but do form disulfide bonds rapidly when they are placed in proximity. In addition, by incorporating these groups at various positions on large RNAs by semisynthesis, the dynamics of thermal motions can be detected. Such motions are believed to be linked to biological function, and the protocols presented in this unit are among the few simple ways to assess such dynamics.<\/jats:p>","DOI":"10.1002\/0471142700.nc0501s00","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T14:00:51Z","timestamp":1086789651000},"source":"Crossref","is-referenced-by-count":1,"title":["Engineering Disulfide Cross\u2010Links in RNA Using Thiol\u2010Disulfide Interchange Chemistry"],"prefix":"10.1002","volume":"00","author":[{"given":"Scott B.","family":"Cohen","sequence":"first","affiliation":[{"name":"University of Colorado  Boulder Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R.","family":"Cech","sequence":"additional","affiliation":[{"name":"University of Colorado  Boulder Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/07328319208021177"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00227a052"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja9707421"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja960091t"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo01326a037"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1589782"},{"key":"e_1_2_8_1_8_1","unstructured":"S. Patai 1974 John Wiley & Sons New York N.Y."},{"key":"e_1_2_8_1_9_1","unstructured":"J. Sambrook E.F. Fritsch T. Maniatis 1989 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."},{"key":"e_1_2_8_1_10_1","first-page":"575","article-title":"Probing RNA tertiary structure: Interhelical cross\u2010linking of the hammerhead ribozyme","volume":"1","author":"Sigurdsson S.T.","year":"1995","journal-title":"RNA"},{"key":"e_1_2_8_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo00801a002"},{"key":"e_1_2_8_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.14.2677"},{"key":"e_1_2_8_2_2_1","unstructured":"CohenandCech 1997. Seeabove."},{"key":"e_1_2_8_3_1_1","unstructured":"Sigurdssonet al. 1995. Seeabove."}],"container-title":["Current Protocols in Nucleic Acid Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F0471142700.nc0501s00","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142700.nc0501s00","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142700.nc0501s00","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142700.nc0501s00","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T07:22:59Z","timestamp":1692516179000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142700.nc0501s00"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1002\/0471142700.nc0501s00"],"URL":"https:\/\/doi.org\/10.1002\/0471142700.nc0501s00","archive":["Portico"],"relation":{},"ISSN":["1934-9270","1934-9289"],"issn-type":[{"value":"1934-9270","type":"print"},{"value":"1934-9289","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}