{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T19:29:50Z","timestamp":1694114990535},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":4778,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Molecular Biology"],"published-print":{"date-parts":[[1988,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protocols in this unit describe procedures for using mixtures of\n<jats:sup>32<\/jats:sup>P\u2010labeled oligonucleotides to screen recombinant DNA clones bound to nitrocellulose filters. A partial amino acid sequence of a protein is used to predict the nucleotide sequence of the gene that would encode it. A mixture of oligonucleotides is chosen that includes all possible nucleotide sequences encoding that amino acid sequence. This mixture of oligonucleotides is then used to screen a recombinant DNA library for the corresponding clones. In some cases however, the exact nucleotide sequence of a desired clone is known and it is possible to use a unique oligonucleotide as a probe.<\/jats:p>","DOI":"10.1002\/0471142727.mb0604s09","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:28:13Z","timestamp":1086794893000},"source":"Crossref","is-referenced-by-count":2,"title":["Using Synthetic Oligonucleotides as Probes"],"prefix":"10.1002","volume":"2","author":[{"given":"Allan","family":"Duby","sequence":"first","affiliation":[{"name":"The University of Texas Health Science Center at Dallas  Dallas Texas"}]},{"given":"Kenneth A.","family":"Jacobs","sequence":"additional","affiliation":[{"name":"Genetics Institute, Inc  Cambridge Massachusetts"}]},{"given":"Anthony","family":"Celeste","sequence":"additional","affiliation":[{"name":"Genetics Institute, Inc  Cambridge Massachusetts"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.1.278"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/313806a0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","unstructured":"Jacobs K.A. Rudersdorf R. Neill S.D. Dougherty J.P. Brown E.L. andFritsch E.F.1988.The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: Application to identifying recombinant DNA clones.Nucl. Acids Res. In press.","DOI":"10.1093\/nar\/16.10.4637"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(85)90276-1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.2.298"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3755547"},{"key":"e_1_2_7_8_1","first-page":"815","article-title":"Polynucleotide kinase from Escherichia coli infected with bacteriophage T4","volume":"2","author":"Richardson C.C.","year":"1971","journal-title":"Nucl. 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