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Retrovirus vectors can also be used in a variety of cells type to investigate regulatory sequences in which a reporter gene and regulatory sequences are carried by the vector and to immortalize or transform primary cells by transduction of oncogenes. For each application, the infection protocol may vary and must often be optimized. Guidelines for infection of cells in some typical in vivo and in vitro experiments are presented in this overview.<\/jats:p>","DOI":"10.1002\/0471142727.mb0914s36","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:28:13Z","timestamp":1086794893000},"source":"Crossref","is-referenced-by-count":5,"title":["Retrovirus Infection of Cells In Vitro and In Vivo"],"prefix":"10.1002","volume":"36","author":[{"given":"Constance","family":"Cepko","sequence":"first","affiliation":[{"name":"Harvard Medical School  Boston Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren","family":"Pear","sequence":"additional","affiliation":[{"name":"University of Pennsylvania  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1242\/dev.110.3.713","article-title":"Cellular migration patterns in the developing mouse cerebral cortex","volume":"110","author":"Austin C.P.","year":"1990","journal-title":"Development"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.61.5.1639-1646.1987"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.22.8897"},{"key":"e_1_2_4_5_1","first-page":"206","article-title":"Survival and retrovirus infection of murine hematopoietic stem cells in vitro: effects of 5\u2010FU and method of infection","volume":"19","author":"Bodine D.M.","year":"1991","journal-title":"Exp. 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