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The amount of protein (or peptide) in the sample is determined based on its ability to compete with a radiolabeled tracer for binding to a specific antibody. A secondary antibody and PEG are used to precipitate the primary antibody\u2010protein complexes. The amount of radioactivity in the complex is inversely proportional to the amount of protein in the sample. Under ideal conditions, an RIA is capable of measuring picograms of a protein (or peptide), but only if the tracer is labeled at a high specific activity. To this end, this unit also presents protocols for radioiodination of proteins and peptides using lactoperoxidase or the Bolton\u2010Hunter reagent.<\/jats:p>","DOI":"10.1002\/0471142727.mb1024s74","type":"journal-article","created":{"date-parts":[[2006,5,30]],"date-time":"2006-05-30T18:04:40Z","timestamp":1149012280000},"source":"Crossref","is-referenced-by-count":2,"title":["Solution Radioimmunoassay of Proteins and Peptides"],"prefix":"10.1002","volume":"74","author":[{"given":"John A.","family":"Smith","sequence":"first","affiliation":[{"name":"University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,5]]},"reference":[{"key":"e_1_2_8_2_1_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1330529"},{"key":"e_1_2_8_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90391-2"},{"key":"e_1_2_8_2_3_1","first-page":"17.1","volume-title":"Handbook of Experimental Immunology, Immunochemistry","author":"Hunter W.M.","year":"1973"},{"key":"e_1_2_8_2_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/194495a0"},{"key":"e_1_2_8_2_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1130299"},{"key":"e_1_2_8_2_6_1","first-page":"147","volume-title":"Methods in Immunodiagnosis","author":"Re R.N.","year":"1980"},{"key":"e_1_2_8_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90703-X"},{"key":"e_1_2_8_2_8_1","first-page":"216","article-title":"Development of a stable radioiodinating reagent to label monoclonal antibodies for radiotherapy of cancer","volume":"30","author":"Wilbur D.S.","year":"1989","journal-title":"J. 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