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Assays employing Ehrlich reagent (DMAB) detect hexosamines and N\u2010acetylhexosamines, including a method for hydrolyzing the glycosidic linkages of the hexosamines and a method for re\u2010N\u2010acetylation. The TBA and DMB assays can be used to quantitate and fractionate free forms of many types of sialic acids. 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