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One of these is a coating antibody, and the other is a derivatized detecting antibody. A support protocol describes chemical labeling of anti\u2010cytokine monoclonal IgG antibodies with the NIP hapten group to produce the detecting antibody. Another support protocol describes a method for producing horseradish peroxidase (HRPO)\u2010conjugated J4, a rat IgG1 anti\u2010NIP monoclonal antibody that confers the anti\u2010NIP specificity to the HRPO detection system used in the immunoenzymetric assay. The method described in this unit has allowed successful measurement of different cytokines in a wide variety of clinical samples including conditioned medium, serum and plasma, ascites, amniotic fluid, and bronchoalveolar lavage fluid.<\/jats:p>","DOI":"10.1002\/0471142735.im0620s13","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":9,"title":["Immunoenzymetric Assay of Mouse and Human Cytokines Using <scp>NIP<\/scp>\u2010Labeled Anti\u2010Cytokine Antibodies"],"prefix":"10.1002","volume":"13","author":[{"given":"John S.","family":"Abrams","sequence":"first","affiliation":[{"name":"DNAX Research Institute  Palo Alto California"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1992.tb01406.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V79.3.688.688"},{"key":"e_1_2_7_4_1","first-page":"4","volume-title":"Immunoassay: A Practical Guide","author":"Chan D.W.","year":"1987"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/1043-4666(92)90082-3"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(94)70077-X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1994.tb00311.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-167635-3.50008-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115449"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.4049\/jimmunol.141.2.483","article-title":"Development and characterization of monoclonal antibodies to murine macrophage colony\u2010stimulating factor","volume":"141","author":"Lokeshwar B.L.","year":"1988","journal-title":"J. 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