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Epidermal Langerhans cells and dermal dendritic cells are the critical antigen\u2010presenting cells in this reaction which initiate sensitization to haptens by presenting antigens to CD4\u2010 and CD8\u2010bearing T lymphocytes which, in turn, secrete cytokines and recruit other cells to the site of the reaction. In the protocol described here, mice are shaved and the skin of their abdomens is exposed to a hapten. After 5 or 6 days (the afferent phase), the baseline ear thickness is measured prior to initiation of the efferent phase. Finally, the ear is treated epicutaneously with the hapten solution and ear thickness is measured in \u223c24\u00a0hr. The magnitude of the ear swelling reaction after allergen treatment reflects the strength of the immune response. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142735.im0402s113","type":"journal-article","created":{"date-parts":[[2016,4,3]],"date-time":"2016-04-03T08:20:29Z","timestamp":1459671629000},"source":"Crossref","is-referenced-by-count":29,"title":["Contact Hypersensitivity"],"prefix":"10.1002","volume":"113","author":[{"given":"Anthony A.","family":"Gaspari","sequence":"first","affiliation":[{"name":"National Cancer Institute  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen I.","family":"Katz","sequence":"additional","affiliation":[{"name":"National Cancer Institute  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan F.","family":"Martin","sequence":"additional","affiliation":[{"name":"Allergy Research Group, Department of Dermatology, Medical Center, University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,4]]},"reference":[{"key":"e_1_2_4_2_1","first-page":"405","article-title":"Contact and delayed hypersensitivity in the mouse. 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