{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T05:40:01Z","timestamp":1693287601440},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,1]],"date-time":"2001-05-01T00:00:00Z","timestamp":988675200000},"content-version":"vor","delay-in-days":455,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the most widely studied lymphokine systems is the T lymphocyte growth factor interleukin 2 (IL\u20102). This unit describes two basic methods for the quantitation and biochemical characterization of IL\u20102 receptors. The first method employs the radioreceptor assay. Support protocols to this technique describe quantification of data via calculations of association and dissociation rates and the Scatchard plot analysis. The second approach detects cell\u2010surface lymphokine receptors by covalently cross\u2010linking IL\u20102 to its receptor. This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross\u2010linking of IL\u20102 to IL\u20102R.<\/jats:p>","DOI":"10.1002\/0471142735.im0601s35","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":0,"title":["Measurement of Lymphokine Receptors"],"prefix":"10.1002","volume":"35","author":[{"given":"John W.","family":"Lowenthal","sequence":"first","affiliation":[{"name":"CSIRO Division of Animal Health  Parkville,Victoria Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas R.","family":"Malek","sequence":"additional","affiliation":[{"name":"University of Miami School of Medicine  Miami Florida"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horacio","family":"Saragovi","sequence":"additional","affiliation":[{"name":"University of Pennsylvania School of Medicine  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.162.2.501"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/7.1.115"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8128231"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.4.1156"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.4.1060"},{"key":"e_1_2_8_1_7_1","doi-asserted-by":"crossref","first-page":"3988","DOI":"10.4049\/jimmunol.135.6.3988","article-title":"High and low affinity IL\u20102 receptors: Analysis by IL\u20102 dissociation rate and reactivity with a monoclonal anti\u2010receptor antibody PC61","volume":"135","author":"Lowenthal J.W.","year":"1985","journal-title":"J. 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Seeabove."}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im0601s35","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T04:53:40Z","timestamp":1693198420000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im0601s35"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1002\/0471142735.im0601s35"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im0601s35","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}