{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T03:10:01Z","timestamp":1692501001463},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This unit describes three procedures for measurement of interleukin\u201021 (IL\u201021). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL\u201021 and IL\u201015 using CFSE. Finally, a method to assess IL\u201021\u2010induced tyrosine phosphorylation of Stat3 in splenic CD8<jats:sup>+<\/jats:sup> T cells using a flow cytometry\u2013based analysis is described. <jats:italic>Curr. Protoc. Immunol<\/jats:italic>. 78:6.30.1\u20106.30.8. \u00a9 2007 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142735.im0630s78","type":"journal-article","created":{"date-parts":[[2007,8,24]],"date-time":"2007-08-24T15:16:35Z","timestamp":1187968595000},"source":"Crossref","is-referenced-by-count":0,"title":["Measurement of Interleukin\u201021"],"prefix":"10.1002","volume":"78","author":[{"given":"Rong","family":"Zeng","sequence":"first","affiliation":[{"name":"National Institutes of Health  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosanne","family":"Spolski","sequence":"additional","affiliation":[{"name":"National Institutes of Health  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren J.","family":"Leonard","sequence":"additional","affiliation":[{"name":"National Institutes of Health  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.2.755"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2006.10.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0202023"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.657"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/35105066"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1688"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.8.4111"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.200360997"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.9.5361"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/35040504"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.7.3600"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1115"},{"key":"e_1_2_7_16_1","first-page":"9016","article-title":"In vivo antitumor activity of interleukin 21 mediated by natural killer cells","volume":"63","author":"Wang G.","year":"2003","journal-title":"Cancer Res."},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041057"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-054973"}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im0630s78","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142735.im0630s78","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im0630s78","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T02:35:56Z","timestamp":1692498956000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im0630s78"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1002\/0471142735.im0630s78"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im0630s78","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]}}}