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This unit describes an assay of ADCC activity that can be used as a test for immunocompetence in effector cells or to test the activity of a monoclonal antibody to mediate ADCC. In this form of cytotoxicity, effector cells with receptors for the Fc portion of immunoglobulin produce target cell lysis by attachment to the Fc portion of antibodies that are bound to target cells via their antigen\u2010combining sites. Therefore, an ADCC assay involves three essential components: labeled target cells, antibodies with specificity for target\u2010cell surface antigens, and effector\u2010cell populations. The basic protocol describes a method of measuring ADCC effector activity in lymphoid cells (peripheral blood mononuclear cells, or PBMC) that employs\n<jats:sup>51<\/jats:sup>Cr\u2010labeled target cells. The three components are mixed in microtiter\u2010plate wells and lysis of the target cells is detected by measuring the release of radioactivity into the cell supernatant. Support protocols describe procedures for preparing anti\u2010target cell antiserum and <jats:sup>51<\/jats:sup>Cr\u2010labeled target cells.<\/jats:p>","DOI":"10.1002\/0471142735.im0727s08","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":7,"title":["<sup>51<\/sup>Cr Release Assay of Antibody\u2010Dependent Cell\u2010Mediated Cytotoxicity (ADCC)"],"prefix":"10.1002","volume":"8","author":[{"given":"David L.","family":"Nelson","sequence":"first","affiliation":[{"name":"National Cancer Institute  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole C.","family":"Kurman","sequence":"additional","affiliation":[{"name":"National Cancer Institute  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deborah E.","family":"Serbousek","sequence":"additional","affiliation":[{"name":"National Cancer Institute  Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1980.29.849"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1983.tb26898.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.6.2165"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio245282a0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(85)90068-1"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(90)90081-Z"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"4704","DOI":"10.4049\/jimmunol.136.12.4704","article-title":"Cytolytic interactions between murine macrophages, tumor cells, and monoclonal antibodies: Characterization of lytic conditions and requirements for effector activation","volume":"136","author":"Johnson W.J.","year":"1986","journal-title":"J. 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