{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T05:40:05Z","timestamp":1693806005941},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,4,15]],"date-time":"2012-04-15T00:00:00Z","timestamp":1334448000000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Natural killer (NK) cells are an important effector cell of innate immunity. Their interaction with susceptible target cells triggers NK cell cytotoxicity and the release of cytokines. Immunofluorescence flow cytometry\u2013based assays are now the preferred methods for measuring NK cell responses. For these assays, assessment is made on NK cells (CD3<jats:sup>\u2013<\/jats:sup>CD56<jats:sup>+<\/jats:sup> CD16<jats:sup>+<\/jats:sup>) within the viable lymphocyte gate, defined by the parameters of size (FSC) and granularity (SSC). Accordingly, NK cells that have not dissociated from target cells at the time of measurement, or that have undergone target cell\u2013induced apoptosis, are excluded from the viable lymphocyte gate and therefore from analysis. This unit describes a protocol for assessing NK cell function in response to target cells (both natural killing and antibody\u2010dependent cell cytotoxicity) based on the loss of NK cells from the lymphocyte gate. This target\u2010induced NK loss (TINKL) should provide a sensitive measure of NK cell responses in a clinical laboratory setting. <jats:italic>Curr. Protoc. Immunol<\/jats:italic>. 97:14.29.1\u201014.29.15. \u00a9 2012 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142735.im1429s97","type":"journal-article","created":{"date-parts":[[2012,4,2]],"date-time":"2012-04-02T16:40:24Z","timestamp":1333384824000},"source":"Crossref","is-referenced-by-count":1,"title":["Target\u2010Induced Natural Killer Cell Loss as a Measure of NK Cell Responses"],"prefix":"10.1002","volume":"97","author":[{"given":"Hilary","family":"Warren","sequence":"first","affiliation":[{"name":"Cancer Immunology Research Unit, The Canberra Hospital  Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,4,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.08.008"},{"key":"e_1_2_7_3_1","first-page":"181","article-title":"Quantitative assay of the lytic action of immune lymphoid cells on 51Cr\u2010labelled allogeneic target cells in vitro: Inhibition by isoantibody and by drugs","volume":"14","author":"Brunner K.T.","year":"1968","journal-title":"Immunology"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.9.6010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1000844"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.3.907"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.23.021704.115526"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2011.06.002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(03)00106-6"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.10.5144"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.9.3254"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.12.5127.bloodjournal87125127"}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F0471142735.im1429s97","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1429s97","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142735.im1429s97","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1429s97","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T16:58:43Z","timestamp":1693760323000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im1429s97"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/0471142735.im1429s97"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im1429s97","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]}}}