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The SCID model is highly reproducible and easily manipulated, and as such provides a useful tool for studying mucosal immune regulation as it relates to the pathogenesis and treatment of IBD in humans. This unit describes a cell transfer system in which SCID mice are reconstituted with CD4<jats:sup>+<\/jats:sup> CD45RB<jats:sup>high<\/jats:sup> cells to induce IBD. The CD4<jats:sup>+<\/jats:sup> cells are isolated by immunomagnetic negative selection using monoclonal antibodies, and are then separated by fluorescent cell sorting into CD45RB<jats:sup>high<\/jats:sup> and CD45RB<jats:sup>low<\/jats:sup> cells. The former population reproducibly induces disease in recipients. A support protocol describes methods to monitor disease progression, which is characterized by weight loss, loose stools, and histologically assessed lesions in the colon.<\/jats:p>","DOI":"10.1002\/0471142735.im1513s30","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":14,"title":["Induction of Inflammatory Bowel Disease in Immunodeficient Mice by Depletion of Regulatory T Cells"],"prefix":"10.1002","volume":"30","author":[{"given":"Simon","family":"Read","sequence":"first","affiliation":[{"name":"John Radcliffe Hospital  Oxford United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona","family":"Powrie","sequence":"additional","affiliation":[{"name":"John Radcliffe Hospital  Oxford United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.7.3464"},{"key":"e_1_2_6_3_1","first-page":"127","article-title":"Interleukin\u20102\u2010 and interferon\u2010gamma\u2010secreting T cells in normal and diseased human intestinal mucosa","volume":"78","author":"Breese E.","year":"1993","journal-title":"Immunology"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.8.3126-3131.1997"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.48757.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1983.tb00446.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)80068-P"},{"key":"e_1_2_6_8_1","first-page":"1503","article-title":"Inflammatory bowel disease in C.B\u201017 scid mice reconstituted with the CD45RBhigh subset of CD4+ T cells","volume":"148","author":"Leach M.W.","year":"1996","journal-title":"Am. 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