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Protocols to measure the bacterial load in the murine kidney following the establishment of pyelonephritis by determining the bacterial colony forming units (CFU) are also included. This assay can be performed with kidney homogenates if the bacterial load is the sole read\u2010out or if cellular intactness is not required for subsequent assays, or from kidney single cell suspensions prepared by enzymatic digestion. A support protocol describes methods for isolating cells for flow cytometry, ex vivo culture, and other assays. <jats:italic>Curr. Protoc. Immunol<\/jats:italic>. 101:15.23.1\u201015.23.9. \u00a9 2013 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142735.im1523s101","type":"journal-article","created":{"date-parts":[[2013,4,5]],"date-time":"2013-04-05T18:22:22Z","timestamp":1365186142000},"source":"Crossref","is-referenced-by-count":3,"title":["Mouse Model for Pyelonephritis"],"prefix":"10.1002","volume":"101","author":[{"given":"Andr\u00e9 P.","family":"Tittel","sequence":"first","affiliation":[{"name":"Institutes of Molecular Medicine and Experimental Immunology (IMMEI), Rheinische Friedrich\u2010Wilhelms University  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Heuser","sequence":"additional","affiliation":[{"name":"Institutes of Molecular Medicine and Experimental Immunology (IMMEI), Rheinische Friedrich\u2010Wilhelms University  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Kurts","sequence":"additional","affiliation":[{"name":"Institutes of Molecular Medicine and Experimental Immunology (IMMEI), Rheinische Friedrich\u2010Wilhelms University  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11908-999-0044-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.7.4773"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00881-06"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.40.1.273-283.1983"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007-2894"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5393.1494"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00729.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1981.20"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3179-3186.2004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5001567"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.12.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.7.1555"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2010101072"}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F0471142735.im1523s101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1523s101","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142735.im1523s101","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1523s101","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T08:53:32Z","timestamp":1692435212000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im1523s101"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/0471142735.im1523s101"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im1523s101","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4]]}}}