{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:55:06Z","timestamp":1792673706346,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,15]],"date-time":"2001-05-15T00:00:00Z","timestamp":989884800000},"content-version":"vor","delay-in-days":896,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[1998,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.<\/jats:p>","DOI":"10.1002\/0471142735.im1902s28","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":40,"title":["Animal Models for the Analysis of Immune Responses to Leishmaniasis"],"prefix":"10.1002","volume":"28","author":[{"given":"David L.","family":"Sacks","sequence":"first","affiliation":[{"name":"National Institute of Allergy &amp; Infectious, Diseases  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Melby","sequence":"additional","affiliation":[{"name":"University of Texas Health Sciences Center  San Antonio Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,5,15]]},"reference":[{"key":"e_1_2_11_1_2_1","first-page":"206","article-title":"The origin of the golden hamster as a laboratory animal","volume":"25","author":"Adler J.H.","year":"1989","journal-title":"Isr. J. Med. Sci."},{"key":"e_1_2_11_1_3_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1983.32.277"},{"key":"e_1_2_11_1_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000076678"},{"key":"e_1_2_11_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/283072a0"},{"key":"e_1_2_11_1_6_1","first-page":"130","article-title":"Regulation of Leishmania populations within the host. II. Genetic control of acute susceptibility of mice to Leishmania donovani infection","volume":"30","author":"Bradley D.J.","year":"1977","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_11_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1636090"},{"key":"e_1_2_11_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(91)90104-5"},{"key":"e_1_2_11_1_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1989.tb01086.x"},{"key":"e_1_2_11_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(76)90069-2"},{"key":"e_1_2_11_1_11_1","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.4049\/jimmunol.134.4.2713","article-title":"Growth cycle dependent generation of complement resistant Leishmania promastigotes","volume":"134","author":"Franke E.D.","year":"1985","journal-title":"J. Immunol."},{"key":"e_1_2_11_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1974.tb03692.x"},{"key":"e_1_2_11_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(90)90039-F"},{"key":"e_1_2_11_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(87)90083-X"},{"key":"e_1_2_11_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.39.3.1087-1094.1983"},{"key":"e_1_2_11_1_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1995.11813015"},{"key":"e_1_2_11_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1980.tb00061.x"},{"key":"e_1_2_11_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(87)90138-X"},{"key":"e_1_2_11_1_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/33.1.2578146"},{"key":"e_1_2_11_1_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1976.tb03763.x"},{"key":"e_1_2_11_1_21_1","first-page":"309","article-title":"A comparative study of the virulence of L. tropica major strains","volume":"34","author":"Kellina O.I.","year":"1965","journal-title":"Med. Parazitol. Parazit. Bolezni."},{"key":"e_1_2_11_1_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1997.4183"},{"key":"e_1_2_11_1_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05443.x"},{"key":"e_1_2_11_1_24_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3100807"},{"key":"e_1_2_11_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.1.18-27.1998"},{"key":"e_1_2_11_1_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1984.14"},{"key":"e_1_2_11_1_27_1","unstructured":"D.H. Molyneux R.W. Ashford 1983 Taylor and Francis London"},{"key":"e_1_2_11_1_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.9.3655-3662.1994"},{"key":"e_1_2_11_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(84)90022-5"},{"key":"e_1_2_11_1_30_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.640"},{"key":"e_1_2_11_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(79)90062-6"},{"key":"e_1_2_11_1_32_1","first-page":"126","article-title":"Experimental cutaneous leishmaniasis. V. Protective immunity in subclinical and self\u2010healing infections in the mouse","volume":"23","author":"Preston P.M.","year":"1976","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_11_1_33_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.3.887"},{"key":"e_1_2_11_1_34_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.623"},{"key":"e_1_2_11_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(89)90176-8"},{"key":"e_1_2_11_1_36_1","first-page":"200","article-title":"The structure and function of the surface lipophosphoglycan on different developmental stages of Leishmania promastigotes","volume":"1","author":"Sacks D.L.","year":"1992","journal-title":"Infect. Agents Disease"},{"key":"e_1_2_11_1_37_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000075727"},{"key":"e_1_2_11_1_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.2.685"},{"key":"e_1_2_11_1_39_1","doi-asserted-by":"publisher","DOI":"10.2307\/3281388"},{"key":"e_1_2_11_1_40_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1982.31.230"},{"key":"e_1_2_11_1_41_1","first-page":"80","article-title":"Host resistance to the Khartoum strain of Leishmania donovani","volume":"45","author":"Stauber L.A.","year":"1958","journal-title":"Rice Inst. Pamph."},{"key":"e_1_2_11_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(66)90002-6"},{"key":"e_1_2_11_1_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.126.4.1614"},{"key":"e_1_2_11_1_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1985.tb00098.x"},{"key":"e_1_2_11_1_45_1","doi-asserted-by":"publisher","DOI":"10.2307\/3282344"},{"key":"e_1_2_11_1_46_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182097002382"},{"key":"e_1_2_11_2_2_1","unstructured":"Hommelet al. 1995. Seeabove."},{"key":"e_1_2_11_2_4_1","unstructured":"Sacks 1992. Seeabove."}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1902s28","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T05:43:31Z","timestamp":1693201411000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im1902s28"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,12]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,12]]}},"alternative-id":["10.1002\/0471142735.im1902s28"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im1902s28","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,12]]}}}