{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:15:58Z","timestamp":1790270158703,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cecum contains a high concentration of microbes, which are a combination of Gram\u2010negative and Gram\u2010positive flora. These bacteria range from anaerobic to facultative aerobic to aerobic organisms. In the procedure described in this unit, the ligation of the cecum produces a source of ischemic tissue as well as polymicrobial infection. This combination of ischemic\/necrotic tissue and microbial infection distinguishes this multifactorial model from a number of other bacterial sepsis models, including but not limited to: bacteremia secondary to intravenous or intraperitoneal administration; fecal administration or intraperitoneal administration of fecal or bacterial plugs; colonic stents; and bacterial abscess formation. <jats:italic>Curr. Protoc. Immunol<\/jats:italic>. 91:19.13.1\u201019.13.11. \u00a9 2010 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471142735.im1913s91","type":"journal-article","created":{"date-parts":[[2010,11,4]],"date-time":"2010-11-04T18:57:48Z","timestamp":1288897068000},"source":"Crossref","is-referenced-by-count":49,"title":["Cecal Ligation and Puncture"],"prefix":"10.1002","volume":"91","author":[{"given":"Alex G.","family":"Cuenca","sequence":"first","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Delano","sequence":"additional","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kindra M.","family":"Kelly\u2010Scumpia","sequence":"additional","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyle L.","family":"Moldawer","sequence":"additional","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip A.","family":"Efron","sequence":"additional","affiliation":[{"name":"University of Florida College of Medicine  Gainesville Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63615-2"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1854"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20062602"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000126648.58732.8c"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e31802b5d9f"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199108000-00008"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000127036.27343.48"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2005.04.006"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000191415.02085.48"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra021333"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199708000-00015"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000191414.94461.7e"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-199903000-00001"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1072-7515(01)01011-0"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022139"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/187221309787158416"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e318181a343"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.4141"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0806542"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.214"},{"key":"e_1_2_3_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.5.3282"},{"key":"e_1_2_3_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7943"},{"key":"e_1_2_3_24_1","unstructured":"Townsend C.M. Jr. Evers B.M. andMattox K.L.2007. Sabiston Textbook of Surgery: Expert Consult Premium Edition: Enhanced Online Features and Print (Sabiston Textbook of Surgery: The Biological Basis of Modern Practicsurgical Practice) Saunders Philadelphia."},{"key":"e_1_2_3_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200304000-00003"},{"key":"e_1_2_3_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000142249.08135.e9"},{"key":"e_1_2_3_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc7088"},{"key":"e_1_2_3_28_1","first-page":"33200","article-title":"Sepsis in the young rat: Maternal milk protects during cecal ligation and puncture sepsis but not during endotoxemia","author":"Witek\u2010Janusek L.","year":"1991","journal-title":"Circ. Shock"},{"key":"e_1_2_3_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-008-1360-9"}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1913s91","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142735.im1913s91","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im1913s91","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T06:28:23Z","timestamp":1693722503000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im1913s91"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1002\/0471142735.im1913s91"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im1913s91","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11]]}}}