{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T02:54:56Z","timestamp":1792896896268,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,5,15]],"date-time":"2001-05-15T00:00:00Z","timestamp":989884800000},"content-version":"vor","delay-in-days":226,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This unit details protocols for in vivo models of subcutaneous growth and pulmonary metastases of B16 melanoma. Therapeutic approaches include the use of B16.GM\u2010CSF and rVVmTRP\u20101 to induce autoimmune vitiligo and tumor protection. The induction and use of gp100\u2010specific therapeutic cytotoxic T lymphocytes (CTL) are discussed. Methods are also included for CTL induction, isolation and testing, CTL maintenance, and adoptive transfer. Support protocols detail the testing of mouse sera for presence of MDA\u2010specific antibodies by immunoblotting and ELISA, respectively. Additional sections, including growing B16 melanoma, enumerating pulmonary metastases, and use of recombinant viruses for vaccination, are discussed together with safety concerns.<\/jats:p>","DOI":"10.1002\/0471142735.im2001s39","type":"journal-article","created":{"date-parts":[[2004,6,9]],"date-time":"2004-06-09T15:13:09Z","timestamp":1086793989000},"source":"Crossref","is-referenced-by-count":249,"title":["B16 as a Mouse Model for Human Melanoma"],"prefix":"10.1002","volume":"39","author":[{"given":"Willem W.","family":"Overwijk","sequence":"first","affiliation":[{"name":"National Cancer Institute, NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas P.","family":"Restifo","sequence":"additional","affiliation":[{"name":"National Cancer Institute, NIH  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,5,15]]},"reference":[{"key":"e_1_2_17_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.3.453"},{"key":"e_1_2_17_3_1","doi-asserted-by":"crossref","first-page":"897","DOI":"10.4049\/jimmunol.161.2.897","article-title":"T cell\u2013mediated, IFN\u2010gamma\u2010facilitated rejection of murine B16 melanomas","volume":"161","author":"Bohm W.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_17_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260135"},{"key":"e_1_2_17_5_1","first-page":"253","article-title":"Genetic vaccination with \u201cself\u201d tyrosinase\u2010related protein 2 causes melanoma eradication but not vitiligo","volume":"60","author":"Bronte V.","year":"2000","journal-title":"Cancer Res."},{"key":"e_1_2_17_6_1","unstructured":"Centers for Disease Control and Prevention 1999"},{"key":"e_1_2_17_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.2.523"},{"key":"e_1_2_17_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.11.21.2822"},{"key":"e_1_2_17_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.7.1221"},{"key":"e_1_2_17_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7513441"},{"key":"e_1_2_17_11_1","doi-asserted-by":"crossref","first-page":"781","DOI":"10.4049\/jimmunol.157.2.781","article-title":"Enhanced growth of murine melanoma in ultraviolet\u2010irradiated skin is associated with local inhibition of immune effector mechanisms","volume":"157","author":"Donawho C.K.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_17_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3539"},{"key":"e_1_2_17_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.9.1553"},{"key":"e_1_2_17_14_1","first-page":"16.16.1","volume-title":"Current Protocols in Molecular Biology","author":"Earl P.L.","year":"1998"},{"key":"e_1_2_17_15_1","first-page":"3945","article-title":"Mechanism of tumor cell resistance to lysis by syngeneic lymphocytes","volume":"37","author":"Fidler I.J.","year":"1977","journal-title":"Cancer Res."},{"key":"e_1_2_17_16_1","first-page":"3160","article-title":"Characterization in vivo and in vitro of tumor cells selected for resistance to syngeneic lymphocyte\u2010mediated cytotoxicity","volume":"36","author":"Fidler I.J.","year":"1976","journal-title":"Cancer Res."},{"key":"e_1_2_17_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1609"},{"key":"e_1_2_17_18_1","first-page":"587","article-title":"The selection and characterization of an invasive variant of the B16 melanoma","volume":"97","author":"Hart I.R.","year":"1979","journal-title":"Am. J. Pathol."},{"key":"e_1_2_17_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.12.2357"},{"key":"e_1_2_17_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/89.21.1595"},{"key":"e_1_2_17_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0749.1995.tb00648.x"},{"key":"e_1_2_17_22_1","doi-asserted-by":"crossref","first-page":"1343","DOI":"10.4049\/jimmunol.155.3.1343","article-title":"Identification of a tyrosinase epitope recognized by HLA\u2010A24\u2010restricted, tumor\u2010infiltrating lymphocytes","volume":"155","author":"Kang X.","year":"1995","journal-title":"J. Immunol."},{"key":"e_1_2_17_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.347"},{"key":"e_1_2_17_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.154.8.3961"},{"key":"e_1_2_17_25_1","doi-asserted-by":"crossref","first-page":"6985","DOI":"10.4049\/jimmunol.161.12.6985","article-title":"Identification of new melanoma epitopes on melanosomal proteins recognized by tumor infiltrating T lymphocytes restricted by HLA\u2010A1, \u2010 A2, and \u2010A3 alleles","volume":"161","author":"Kawakami Y.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_17_26_1","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.4049\/jimmunol.160.5.2099","article-title":"Human melanoma patients recognize an HLA\u2010A1\u2010restricted CTL epitope from tyrosinase containing two cysteine residues: Implications for tumor vaccine development","volume":"160","author":"Kittlesen D.J.","year":"1998","journal-title":"J. Immunol."},{"key":"e_1_2_17_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980504)76:3<430::AID-IJC23>3.0.CO;2-D"},{"key":"e_1_2_17_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.25.14809"},{"key":"e_1_2_17_29_1","first-page":"5157","article-title":"c\u2010Met autocrine activation induces development of malignant melanoma and acquisition of the metastatic phenotype","volume":"58","author":"Otsuka T.","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_17_30_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.1997.0461"},{"key":"e_1_2_17_31_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.2.277"},{"key":"e_1_2_17_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.2982"},{"key":"e_1_2_17_33_1","first-page":"4895","article-title":"Identification of a shared HLA\u2010A*0201\u2010restricted T\u2010cell epitope from the melanoma antigen tyrosinase\u2010related protein 2 (TRP2)","volume":"58","author":"Parkhurst M.R.","year":"1998","journal-title":"Cancer Res."},{"key":"e_1_2_17_34_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.159.1.303"},{"key":"e_1_2_17_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)84664-6"},{"key":"e_1_2_17_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80028-X"},{"key":"e_1_2_17_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-199601000-00009"},{"key":"e_1_2_17_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19980130)75:3<451::AID-IJC20>3.0.CO;2-A"},{"key":"e_1_2_17_39_1","doi-asserted-by":"crossref","first-page":"5027","DOI":"10.4049\/jimmunol.157.11.5027","article-title":"Shared epitopes for HLA\u2010A3\u2010restricted melanoma\u2010reactive human CTL include a naturally processed epitope from Pmel\u201017\/gp100","volume":"157","author":"Skipper J.C.","year":"1996","journal-title":"J. Immunol."},{"key":"e_1_2_17_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.5.1965"},{"key":"e_1_2_17_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.7.3535"},{"key":"e_1_2_17_42_1","doi-asserted-by":"crossref","first-page":"1796","DOI":"10.4049\/jimmunol.158.4.1796","article-title":"Identification of subdominant CTL epitopes of the GP100 melanoma\u2010 associated tumor antigen by primary in vitro immunization with peptide\u2010 pulsed dendritic cells","volume":"158","author":"Tsai V.","year":"1997","journal-title":"J. Immunol."},{"key":"e_1_2_17_43_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.3.355"},{"key":"e_1_2_17_44_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.184.6.2207"},{"key":"e_1_2_17_45_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.1131"},{"key":"e_1_2_17_46_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI4004"},{"key":"e_1_2_17_47_1","first-page":"613","article-title":"The tyrosinase gene family\u2014interactions of melanogenic proteins to regulate melanogenesis","volume":"40","author":"Winder A.","year":"1994","journal-title":"Cell Mol. Biol. Res."},{"key":"e_1_2_17_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240340"},{"key":"e_1_2_17_49_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.5.1415"},{"key":"e_1_2_17_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.2.989"},{"key":"e_1_2_17_51_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00133.x"}],"container-title":["Current Protocols in Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im2001s39","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142735.im2001s39","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142735.im2001s39","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T19:54:17Z","timestamp":1693770857000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142735.im2001s39"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1002\/0471142735.im2001s39"],"URL":"https:\/\/doi.org\/10.1002\/0471142735.im2001s39","archive":["Portico"],"relation":{},"ISSN":["1934-3671","1934-368X"],"issn-type":[{"value":"1934-3671","type":"print"},{"value":"1934-368X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}