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Resting peripheral blood lymphocytes do not normally express any appreciable levels of CD154, necessitating in vitro activation. This unit details the in vitro activation procedure, the three\u2010color monoclonal antibody panels used to label the appropriate lymphocyte subsets, the controls required to interpret the assay, the setup and acquisition of listmode data files, and the gating and analysis protocols used to interpret the data.<\/jats:p>","DOI":"10.1002\/0471142956.cy0607s12","type":"journal-article","created":{"date-parts":[[2004,6,8]],"date-time":"2004-06-08T21:01:36Z","timestamp":1086728496000},"source":"Crossref","is-referenced-by-count":1,"title":["Measurement of <scp>CD<\/scp>40 Ligand (<scp>CD<\/scp>154) Expression on Resting and In Vitro\u2013Activated T Cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Maurice R.G.","family":"O'Gorman","sequence":"first","affiliation":[{"name":"Northwestern University Medical School, Children's Memorial Hospital  Chicago Illinois"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,5]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7679801"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/357080a0"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.150.9.3671"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90668-G"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.001243"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/361541a0"},{"key":"e_1_2_4_8_1","first-page":"101","article-title":"Evaluation of CD154 expression on the surface of CD4+ lymphocytes obtained from pediatric HIV\u20101 infected patients","volume":"9","author":"DuChateau B.K.","year":"1998","journal-title":"Cytometry"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.3.1099"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.6.2170"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5283.1864"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05530.x"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/361539a0"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/342929a0"},{"key":"e_1_2_4_15_1","first-page":"101","article-title":"Immunodeficiency with hyper IgM (HIM)","volume":"3","author":"Notarangelo L.D.","year":"1992","journal-title":"Immunodef. 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