{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T19:28:17Z","timestamp":1694114897465},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,5,1]],"date-time":"2005-05-01T00:00:00Z","timestamp":1114905600000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Cytometry"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic lymphocytic leukemia (CLL) is the most common chronic leukemia in Western countries. The disease has an extremely variable clinical course, and several prognostic features have been identified to assess individual risk. The configuration of the immunoglobulin variable heavy\u2010chain gene (IgV<jats:sub>H<\/jats:sub>) is a strong predictor of the outcome. CLL patients with unmutated IgV<jats:sub>H<\/jats:sub> status have an aggressive clinical course and a short survival. Unfortunately, analysis of IgV<jats:sub>H<\/jats:sub> gene configuration is not available in most clinical laboratories. A small number of genes are differentially expressed between unmutated IgV<jats:sub>H<\/jats:sub> and mutated IgV<jats:sub>H<\/jats:sub> clinical forms of CLL. One of these genes is <jats:italic>ZAP\u201070<\/jats:italic>, which is detected in leukemic cells from patients with the unmutated IgV<jats:sub>H<\/jats:sub> form of CLL. Flow cytometry presents advantages over other methods to detect ZAP\u201070, and its quantification by flow cytometry has proved its predictive value. This unit focuses on protocols to quantify ZAP\u201070 by flow cytometry in CLL.<\/jats:p>","DOI":"10.1002\/0471142956.cy0619s32","type":"journal-article","created":{"date-parts":[[2005,5,24]],"date-time":"2005-05-24T13:19:32Z","timestamp":1116940772000},"source":"Crossref","is-referenced-by-count":1,"title":["<scp>ZAP<\/scp>\u201070 Staining in Chronic Lymphocytic Leukemia"],"prefix":"10.1002","volume":"32","author":[{"given":"Neus","family":"Villamor","sequence":"first","affiliation":[{"name":"Hospital Cl\u00ednic de Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2004.1.163"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1727"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1683"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-05-1715"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa023143"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1840"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403147"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1848"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.thj.6200139"},{"key":"e_1_2_5_11_1","first-page":"127","volume-title":"World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of Haematopoietic and Lymphoid Tissues","author":"M\u00fcller\u2010Hermelink H.K.","year":"2001"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)15260-9"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040857"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.11.1639"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199510193331606"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3306"}],"container-title":["Current Protocols in Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142956.cy0619s32","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471142956.cy0619s32","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471142956.cy0619s32","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T19:36:26Z","timestamp":1693769786000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471142956.cy0619s32"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,4]]}},"alternative-id":["10.1002\/0471142956.cy0619s32"],"URL":"https:\/\/doi.org\/10.1002\/0471142956.cy0619s32","archive":["Portico"],"relation":{},"ISSN":["1934-9297","1934-9300"],"issn-type":[{"value":"1934-9297","type":"print"},{"value":"1934-9300","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4]]}}}