{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T06:06:16Z","timestamp":1704261976817},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Cytometry"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HLA\u2010B27 is common to the entire group of seronegative spondyloarthropathies, and assessment of HLA\u2010B27 is of diagnostic importance if any of these diseases are considered. Among the alternatives to traditional typing by the complement\u2010dependent cytotoxicity assay, flow cytometric HLA\u2010B27 screening is most widely used in general diagnostic laboratories, as it is simple, rapid, and cost effective. This unit describes screening for HLA\u2010B27 on peripheral blood lymphocytes using more than one HLA\u2010B27 monoclonal antibody to detect possible cross\u2010reactivity with non\u2010HLA\u2010B27 antigens. Screening is hampered by the lack of true monospecific anti\u2010HLA\u2010B27 monoclonal antibodies. Cross\u2010reactivities of anti\u2010HLA\u2010B27 with other HLA\u2010B antigens have been reported. The authors recommend use of GS145.2 and FD705 antibodies, as this combination avoids most false\u2010positive conclusions. Lyse\u2010and\u2010wash sample processing is employed. A multiparameter flow methodology is applied. Three to four parameters\u2013forward scatter, side scatter, HLA\u2010B27 expression, and, in some cases CD3 or B7 expression\u2013are acquired.<\/jats:p>","DOI":"10.1002\/0471142956.cy0622s33","type":"journal-article","created":{"date-parts":[[2005,8,31]],"date-time":"2005-08-31T18:16:50Z","timestamp":1125512210000},"source":"Crossref","is-referenced-by-count":3,"title":["Flow Cytometric Screening for the <scp>HLA<\/scp>\u2010B27 Antigen on Peripheral Blood Lymphocytes"],"prefix":"10.1002","volume":"33","author":[{"given":"Wilfried H.B.M.","family":"Levering","sequence":"first","affiliation":[{"name":"Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood  Bank South West Region Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kees","family":"Sintnicolaas","sequence":"additional","affiliation":[{"name":"Laboratory for Histocompatibility and Immunogenetics, Sanquin Blood  Bank South West Region Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henk","family":"Wind","sequence":"additional","affiliation":[{"name":"Erasmus MC  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Hooijkaas","sequence":"additional","affiliation":[{"name":"Erasmus MC  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan W.","family":"Gratama","sequence":"additional","affiliation":[{"name":"Erasmus MC  Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,8]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1093\/clinchem\/33.9.1619","article-title":"HLA\u2010B27 typing by use of flow cytofluorometry","volume":"33","author":"Albrecht J.","year":"1987","journal-title":"Clin. 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