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These granules are modified lysosomes containing preformed mediators such as histamine, neutral proteases, cytokines, and growth factors embedded in a heparin proteoglycan matrix. When exposed to the extracellular fluid, the soluble components of the granules (e.g., histamine and cytokines) diffuse away, whereas the heparin proteoglycans and the mast cell\u2013specific neutral proteases (e.g., chymase) remain tightly bound to each other, forming proteolytically active intra\u2010 and extracellular granule remnants. This unit describes a method to isolate rat serosa mast cell granule remnants in experiments aimed at determining the role of mast cell activation and degranulation in disease.<\/jats:p>","DOI":"10.1002\/0471143030.cb0316s29","type":"journal-article","created":{"date-parts":[[2006,1,17]],"date-time":"2006-01-17T16:18:11Z","timestamp":1137514691000},"source":"Crossref","is-referenced-by-count":3,"title":["Isolation of Mast Cell Granules"],"prefix":"10.1002","volume":"29","author":[{"given":"Ken A.","family":"Lindstedt","sequence":"first","affiliation":[{"name":"Wihuri Research Institute  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petri T.","family":"Kovanen","sequence":"additional","affiliation":[{"name":"Wihuri Research Institute  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.111.3.909"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90517-2"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1972.tb05183.x"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38637-4"},{"key":"e_1_2_13_6_1","first-page":"257","volume-title":"Mast Cell Proteases in Immunology and Biology. 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