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The protocols in this unit take advantage of the large size (up to 1 \u00b5m diameter) and high density (&gt;1.20 g\/cm<jats:sup>3<\/jats:sup> on sucrose gradients) of the granules as compared to other cellular organelles. They use a combination of differential sedimentation and density gradient separation to accomplish the purification. Similar procedures can be used to isolate zymogen granules from mouse pancreas and canine pancreas. A protocol for preparing zymogen granules from dog pancreas is also included.<\/jats:p>","DOI":"10.1002\/0471143030.cb0318s29","type":"journal-article","created":{"date-parts":[[2006,1,17]],"date-time":"2006-01-17T16:18:11Z","timestamp":1137514691000},"source":"Crossref","is-referenced-by-count":0,"title":["Isolation of Zymogen Granules from Rat Pancreas"],"prefix":"10.1002","volume":"29","author":[{"given":"Michael J.","family":"Rindler","sequence":"first","affiliation":[{"name":"New York University School of Medicine  New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.38.1.49"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.4.1299"},{"key":"e_1_2_10_4_1","first-page":"G411","article-title":"Isolation of stable pancreatic zymogen granules","volume":"246","author":"Lisle R.C.","year":"1984","journal-title":"Am. 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