{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:29:30Z","timestamp":1789644570087,"version":"4.0.0"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T00:00:00Z","timestamp":1524614400000},"content-version":"vor","delay-in-days":1697,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Cell Biology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"Abstract<\/jats:title>For more than 100 years, the ultimate resolution of a light microscope (\u223c200 nm) has been constrained by the fundamental physical phenomenon of diffraction, as described by Ernst Abbe in 1873. While this limitation is just as applicable to today's light microscopes, it is the combination of high\u2010end optics, clever methods of sample illumination, and computational techniques that has enabled researchers to access information at an order of magnitude greater resolution than once thought possible. This combination, broadly termed superresolution microscopy, has been increasingly practical for many labs to implement from both a hardware and software standpoint, but, as with many cutting\u2010edge techniques, it also comes with limitations. One of the current drawbacks to superresolution microscopy is the limited number of probes and conditions that have been suitable for imaging. Here, a technique termed bleaching\/blinking\u2010assisted localization microscopy (BaLM) makes use of the inherent blinking and bleaching properties of almost all fluorophores as a means to generate superresolution images. Curr. Protoc. Cell Biol<\/jats:italic>. 60:21.8.1\u201021.8.17. \u00a9 2013 by John Wiley & Sons, Inc.<\/jats:p>","DOI":"10.1002\/0471143030.cb2108s60","type":"journal-article","created":{"date-parts":[[2013,10,22]],"date-time":"2013-10-22T15:40:41Z","timestamp":1382456441000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Superresolution Imaging with Standard Fluorescent Probes"],"prefix":"10.1002","volume":"60","author":[{"given":"Bryan A.","family":"Millis","sequence":"first","affiliation":[{"name":"Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan T.","family":"Burnette","sequence":"additional","affiliation":[{"name":"The Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Lippincott\u2010Schwartz","sequence":"additional","affiliation":[{"name":"The Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bechara","family":"Kachar","sequence":"additional","affiliation":[{"name":"Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders National Institutes of Health Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1127344"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2205"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1117430109"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401638101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2818.2000.00710.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physchem-032210-103340"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0510-339"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.091116"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.15.8206"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118382905.ch1"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth929"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/nl203560r"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)75618-X"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084398"}],"container-title":["Current Protocols in Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2F0471143030.cb2108s60","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471143030.cb2108s60","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/0471143030.cb2108s60","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471143030.cb2108s60","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T12:11:20Z","timestamp":1692447080000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471143030.cb2108s60"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1002\/0471143030.cb2108s60"],"URL":"https:\/\/doi.org\/10.1002\/0471143030.cb2108s60","archive":["Portico"],"relation":{},"ISSN":["1934-2500","1934-2616"],"issn-type":[{"value":"1934-2500","type":"print"},{"value":"1934-2616","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"2018-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}