{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:36:12Z","timestamp":1761662172533},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,1]],"date-time":"2004-10-01T00:00:00Z","timestamp":1096588800000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Cell Biology"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Traditional FISH analysis has employed, at most, two colors of detection, a red\u2010fluorescing fluorochrome and a green\u2010fluorescing fluorochrome. The improvements in fluorescent imaging and development of heartier fluorochromes\/dyes have enabled investigators to use several different DNA probes in one experiment. This may involve all or combinations of locus\u2010specific probes and chromosome paints. The value of such experiments lies in the investigator obtaining far more information from one specific cell at one time, rather then carrying out separate experiments on multiple specimens prepared from the same sample, then extrapolating results. The generic term for multi\u2010color FISH assays is M\u2010FISH, however, the technologies behind the manner in which the fluorochrome information is generated has spawned two different M\u2010FISH systems: spectral karyotyping (SKY) and M\u2010FISH. For both assays, the experimental procedures are identical: commercially available probes for all 24 (human) chromosomes are differentially labeled according to a labeling scheme and hybridized to metaphase spreads for 24 to 48 hr, followed by post\u2010hybridization washes and, if required, antibody detection. The difference lies in the imaging: spectral karyotyping identifies the differentiation of the chromosomes based on their spectral properties, whereas M\u2010FISH identifies the differentiation of the chromosomes based on that fluorochrome's presence or absence when visualized with specific filters. The resulting analysis for both methods is the same, revealing hidden translocations and insertions as well as the chromosomal components of marker chromosomes.<\/jats:p>","DOI":"10.1002\/0471143030.cb2205s24","type":"journal-article","created":{"date-parts":[[2004,9,30]],"date-time":"2004-09-30T14:00:24Z","timestamp":1096552824000},"source":"Crossref","is-referenced-by-count":16,"title":["Multi\u2010Color <scp>FISH<\/scp> Techniques"],"prefix":"10.1002","volume":"24","author":[{"given":"Jane","family":"Bayani","sequence":"first","affiliation":[{"name":"Ontario Cancer Institute  Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Squire","sequence":"additional","affiliation":[{"name":"Ontario Cancer Institute  Toronto Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","unstructured":"Gray J.W. Lukas J. Peters D. Pinkel D. Trask B. Van denEngh G. andVanDilla M.1986.Flow karyotyping and sorting of human chromosomes.InProceedings of Cold Spring Harbor Symposium in Quantitative Biology 51 Pt. 1:141\u2010149.Cold Spring Harbor Laboratory Press Cold Spring Harbor New York.","DOI":"10.1101\/SQB.1986.051.01.017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1996.tb05663.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5274.494"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0496-368"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870040311"}],"container-title":["Current Protocols in Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/0471143030.cb2205s24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T03:10:39Z","timestamp":1693192239000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/0471143030.cb2205s24"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9]]},"references-count":5,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1002\/0471143030.cb2205s24"],"URL":"https:\/\/doi.org\/10.1002\/0471143030.cb2205s24","archive":["Portico"],"relation":{},"ISSN":["1934-2500","1934-2616"],"issn-type":[{"value":"1934-2500","type":"print"},{"value":"1934-2616","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9]]}}}