{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T14:10:04Z","timestamp":1781791804812,"version":"3.54.5"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T00:00:00Z","timestamp":1696896000000},"content-version":"vor","delay-in-days":1012,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":271,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>In this study, we aimed to establish a method for quantifying bromide ions (Br\u2212) in blood and urine using gas chromatograph-mass spectrometer (GC-MS) equipped with a headspace sampler, for biological monitoring of workers exposed to methyl bromide.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Samples were mixed with dimethyl sulfate, and Br\u2212 ions were detected using GC-MS with a headspace sampler. The validity of the proposed method was evaluated based on most of the US FDA guidance. The values obtained were compared with reference values by analysis using SeronormTM Trace Elements Whole Blood L-1 RUO.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The calibration curve showed good linearity in the Br\u2212 concentration range of 0.1\u221220.0 mg\/L, and the coefficient of determination R2 value was &amp;gt;.999. Intraday and interday accuracy values were 99.3%\u2212103.1% and 97.4%\u2212101.8%, respectively. The measured and reference values of Seronorm were concordant. Herein, eight urine and serum samples of workers were analyzed; the samples\u2019 Br\u2212 concentrations were known. The correlation coefficients of urine and serum samples were 0.97 and 0.96, respectively, and results were consistent.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study established a simple and rapid method for the determination of Br\u2212 concentration in biological samples using GC-MS with a headspace sampler. Moreover, it can be used for biological monitoring of occupational exposure to methyl bromide and for the determination of Br\u2212 concentration in a wide range of biological samples.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1002\/1348-9585.12274","type":"journal-article","created":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T03:19:07Z","timestamp":1632971947000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Quantification of bromide ion in biological samples using headspace gas chromatography\u2013mass spectrometry"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-3234","authenticated-orcid":false,"given":"Takenori","family":"Yamauchi","sequence":"first","affiliation":[{"name":"Department of Health and Preventive Medicine, School of Medicine, Showa University , Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daichi","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Pharmaceutical Education Center, Yokohama University of Pharmacy , Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeko","family":"Okubo","sequence":"additional","affiliation":[{"name":"Department of Health and Preventive Medicine, School of Medicine, Showa University , Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akatsuki","family":"Kokaze","sequence":"additional","affiliation":[{"name":"Department of Health and Preventive Medicine, School of Medicine, Showa University , Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Yamano","sequence":"additional","affiliation":[{"name":"Department of Health and Preventive Medicine, School of Medicine, Showa University , Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"issue":"5","key":"2024071808215343900_joh212274-bib-0001","doi-asserted-by":"crossref","DOI":"10.1539\/sangyoeisei.KJ00001991731","article-title":"Two cases of methyl bromide poisoning in termite exterminators","volume":"43","author":"Yamano","year":"2001","journal-title":"Sangyo Eiseigaku Zasshi"},{"issue":"1","key":"2024071808215343900_joh212274-bib-0002","doi-asserted-by":"crossref","first-page":"133","DOI":"10.2486\/indhealth.MS1143","article-title":"Occupational exposure in methyl bromide manufacturing workers: 17-year follow-up study of urinary bromide ion concentration for biological monitoring","volume":"49","author":"Yamano","year":"2011","journal-title":"Ind Health"},{"issue":"4","key":"2024071808215343900_joh212274-bib-0003","first-page":"222","article-title":"Health hazards and countermeasures due to chemical substances 1 methyl bromide","volume":"63","author":"Yamano","year":"2008","journal-title":"Digest of Science of Labour"},{"issue":"10","key":"2024071808215343900_joh212274-bib-0004","doi-asserted-by":"crossref","first-page":"585","DOI":"10.2116\/bunsekikagaku.32.10_585","volume":"32","author":"Suzuki","year":"1983","journal-title":"Bunseki Kagaku"},{"issue":"3","key":"2024071808215343900_joh212274-bib-0005","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1016\/S0021-9673(00)83065-4","article-title":"Methylation of inorganic anions for gas chromatographic determination","volume":"211","author":"Funazo","year":"1981","journal-title":"J Chromatogr A"},{"issue":"6","key":"2024071808215343900_joh212274-bib-0006","doi-asserted-by":"crossref","first-page":"335","DOI":"10.2116\/bunsekikagaku.34.6_335","article-title":"Simple determination of bromide ion in urine by head space gas chromatography","volume":"34","author":"Sugiyama","year":"1985","journal-title":"Bunseki Kagaku"},{"issue":"3","key":"2024071808215343900_joh212274-bib-0007","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1539\/joh1959.29.196","article-title":"A simple determination method of bromide ion in plasma of methyl bromide workers by head space gas chromatography (in Japanese)","volume":"29","author":"Yamano","year":"1987","journal-title":"Sangyo Igaku"},{"key":"2024071808215343900_joh212274-bib-0008","volume-title":"Guidance for industry, bioanalytical method validation","author":"U.S. Department of Health and Human Services, Food and Drug Administration"},{"key":"2024071808215343900_joh212274-bib-0009","volume-title":"Industrial safety and health law","author":"Japan International Center for Occupational Safety and Health"},{"issue":"1","key":"2024071808215343900_joh212274-bib-0010","article-title":"Recommendation of occupational exposure limits (2020\u20132021)","volume":"2","author":"The Japan Society for Occupational Health","year":"2020","journal-title":"Environ Occup Health Practice"},{"key":"2024071808215343900_joh212274-bib-0011","volume-title":"Administrative control level","author":"Japan Industrial Safety and Health Association"},{"issue":"1","key":"2024071808215343900_joh212274-bib-0012","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1007\/s002449900218","article-title":"Diffusive sampling and biological monitoring of 2-bromopropane","volume":"33","author":"Kawai","year":"1997","journal-title":"Arch Environ Contam Toxicol"},{"issue":"2","key":"2024071808215343900_joh212274-bib-0013","doi-asserted-by":"crossref","first-page":"151","DOI":"10.7888\/juoeh.24.151","article-title":"Effects of inhaled methyl bromide gas on the metabolic system and kinetics of bromine ion in rats","volume":"24","author":"Hori","year":"2002","journal-title":"J UOEH"},{"issue":"2","key":"2024071808215343900_joh212274-bib-0014","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/j.jchromb.2004.12.017","article-title":"Determination of bromide in whole blood and urine from humans using gas chromatography-mass spectrometry","volume":"817","author":"Kage","year":"2005","journal-title":"J Chromatogr B Analyt Technol Biomed Life Sci"}],"container-title":["Journal of Occupational Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/1348-9585.12274","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/1348-9585.12274","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/joh\/article-pdf\/63\/1\/e12274\/58562689\/joh212274.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/joh\/article-pdf\/63\/1\/e12274\/58562689\/joh212274.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T08:22:51Z","timestamp":1721290971000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/joh\/article\/doi\/10.1002\/1348-9585.12274\/7249850"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1,10]]}},"URL":"https:\/\/doi.org\/10.1002\/1348-9585.12274","relation":{},"ISSN":["1341-9145","1348-9585"],"issn-type":[{"value":"1341-9145","type":"print"},{"value":"1348-9585","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,1]]},"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2021-04-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12274"}}