{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T22:25:03Z","timestamp":1783463103460,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T00:00:00Z","timestamp":1779062400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005009","name":"Stiftelsen Assar Gabrielssons Fond","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Blood &amp; Cancer"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      An internal tandem duplication in the gene encoding Fms\u2010like tyrosine kinase 3 (\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD) is associated with high relapse risk and poor prognosis in acute myeloid leukemia (AML) and plays a crucial role in treatment decisions. Measurable residual disease (MRD) analysis of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD during and after treatment has shown prognostic value in adults, but its clinical value in children remains unexplored.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedure<\/jats:title>\n                    <jats:p>\n                      This study evaluated the potential of MRD analysis with deep sequencing (ultrahigh\u2010sensitivity next\u2010generation sequencing; UHS\u2010NGS) of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD to assess treatment response and predict impending relapse in children with AML. A total of 168 samples from 17 patients (median age 11\u00a0years, range: 4\u201317) were analyzed. Bone marrow samples were used for evaluation of treatment response, and peripheral blood samples for post\u2010treatment monitoring.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Deep sequencing enabled monitoring of the kinetics of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD subclones throughout treatment. When used for post\u2010treatment monitoring, deep sequencing detected increasing levels of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD in peripheral blood only in patients who relapsed, while no such increase was detected in patients in continuous complete remission. Comparisons with flow cytometry and deep sequencing of other mutations confirmed the sensitivity of the assay and the often subclonal nature of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Deep sequencing of\n                      <jats:italic>FLT3<\/jats:italic>\n                      \u2010ITD is a highly sensitive method for MRD monitoring in children with AML, with significant potential for assessing treatment response and detecting imminent relapse at an early stage. It may serve as a valuable molecular MRD method, especially as a complement to flow cytometry.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/1545-5017.70397","type":"journal-article","created":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T08:03:00Z","timestamp":1779091380000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deep Sequencing of\n                    <i>FLT3<\/i>\n                    \u2010ITD Enables Response Evaluation and Post\u2010Treatment Monitoring in Childhood AML: An Exploratory Study"],"prefix":"10.1002","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5868-0487","authenticated-orcid":false,"given":"Sofie Johansson","family":"Alm","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Institute of Biomedicine Sahlgrenska Academy at University of Gothenburg Gothenburg 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