{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:15:56Z","timestamp":1773382556732,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019971","name":"Luzhou Science and Technology Bureau","doi-asserted-by":"publisher","award":["2022\u2010SYF\u201059"],"award-info":[{"award-number":["2022\u2010SYF\u201059"]}],"id":[{"id":"10.13039\/501100019971","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Ther Apher Dial"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The present study aims to explore the role of nafamostat mesylate (NM) in ischemia\u2013reperfusion (I\/R)\u2010induced acute kidney injury (AKI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010one male rats were randomly divided into three groups: sham, I\/R, and I\/\n                      <jats:italic>R<\/jats:italic>\n                      \u2009+\u2009NM groups. Acute renal I\/R injury models were created in the I\/R and I\/\n                      <jats:italic>R<\/jats:italic>\n                      \u2009+\u2009NM groups by right nephrectomy and clamping the left renal pedicle for 45\u2009min. Rats in the I\/\n                      <jats:italic>R<\/jats:italic>\n                      \u2009+\u2009NM group were intraperitoneally injected with 0.75\u2009mg\/kg of NM before modeling. Blood and kidney specimens were collected at 24\u2009h after model establishment. RNA sequencing (RNA\u2010seq), KEGG and GO enrichment analyses, and single\u2010cell sequencing were carried out to investigate the related mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The pretreatment with NM improved renal function, reduced mitochondrial damage, suppressed the accumulation of reactive oxygen species, and inhibited ferroptosis. The RNA\u2010seq indicated that NM induced protection by upregulating fatty acids, inhibiting inflammation, and promoting DNA repair. Proximal tubular cells were mainly affected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>NM protects against AKI by reducing reactive oxygen species, upregulating fatty acids, and regulating ferroptosis signaling pathways in proximal tubular cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/1744-9987.70112","type":"journal-article","created":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T04:22:22Z","timestamp":1766550142000},"page":"227-244","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nafamostat Mesylate Protects Against Acute Renal Ischemia\u2013Reperfusion Injury by Alleviating Mitochondrial Dysfunction, Inhibiting Ferroptosis, and Regulating Proximal Tubular Cells: A Multi\u2010Omics Analysis"],"prefix":"10.1002","volume":"30","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Southwest Medical University\/Sichuan Clinical Research Center for Nephropathy\/Metabolic Vascular Disease Key Laboratory  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6938-9009","authenticated-orcid":false,"given":"Yuxuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Southwest Medical University\/Sichuan Clinical Research Center for Nephropathy\/Metabolic Vascular Disease Key Laboratory  Luzhou China"},{"name":"The Medical Center of Dujiangyan  Chengdu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongqian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology Affiliated Hospital of Southwest Medical University\/Sichuan Clinical 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