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Kinetic constants, <jats:italic>k<\/jats:italic><jats:sub>on<\/jats:sub> = 1.2 \u00d7 10<jats:sup>5<\/jats:sup> <jats:sc>m<\/jats:sc><jats:sup>\u22121<\/jats:sup>\u00b7s<jats:sup>\u22121<\/jats:sup> and <jats:italic>k<\/jats:italic><jats:sub>off<\/jats:sub> = 2.5 \u00d7 10<jats:sup>\u22124<\/jats:sup>\u00b7s<jats:sup>\u22121<\/jats:sup>, have been determined and compared with those for mammalian hemoglobins. Density Functional Theory study supports the binding of H<jats:sub>2<\/jats:sub>S by modeling the configurations of HOMO dispersions. We hypothesized that VHb is involved in H<jats:sub>2<\/jats:sub>S reception and storage. Different from <jats:italic>Lucina pectinata<\/jats:italic> HbI, a typical H<jats:sub>2<\/jats:sub>S\u2010binding hemoglobin, VHb, exhibits unusual properties on H<jats:sub>2<\/jats:sub>S reactivity such as steric constraints playing an important role in modulating H<jats:sub>2<\/jats:sub>S entry. A distinct mechanism of VHb interaction with H<jats:sub>2<\/jats:sub>S is supported by studies of variant forms of VHb.<\/jats:p>","DOI":"10.1002\/1873-3468.12141","type":"journal-article","created":{"date-parts":[[2016,3,19]],"date-time":"2016-03-19T14:47:35Z","timestamp":1458398855000},"page":"1132-1142","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Clues for discovering a new biological function of <i>Vitreoscilla<\/i> hemoglobin in organisms: potential sulfide receptor and storage"],"prefix":"10.1002","volume":"590","author":[{"given":"Dandan","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haoran","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Physics Georgia Southern University Statesboro GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengqiang","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education College of Life Science Jilin University Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1139\/o82-076"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0351138"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.11.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.10.019"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4345"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2004.10.012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi400745a"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)46185-0"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi801738j"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0228"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.11.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.16.9517"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01733907"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(97)00206-2"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2006.11.001"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)96464-1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)35680-6"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/331633a0"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1994.tb19931.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2013.07.005"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070668"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOPC.0000027848.40972.97"},{"key":"e_1_2_7_24_1","first-page":"184","article-title":"High\u2010level production of recombinant sulfide\u2010reactive hemoglobin I from Lucina pectinata in Escherichia coli. 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